BMP-2/BMP-4 Antibody [Biotin]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

BMP-2/BMP-4 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human BMP‑2
  Ala284-Arg396
  Accession # P12643
• Specificity: Detects human BMP‑2/BMP‑4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BMP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-2/BMP-4 Antibody [Biotin]

• BDA2
• BMP-2
• BMP-2/BMP-4
• BMP2A
• BMP-2A
• BMP2ABone morphogenetic protein 2A
• bone morphogenetic protein 2

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
2. Chen, D. et al. (2004) Growth Factors 22:233.
3. Wozney, J. et al. (1988) Science 242:1528.
4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
6. De Luca, F. et al. (2001) Endocrinology 142:430.
7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
8. Ryoo, H.-M. et al. (2006) Gene 366:51.
9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
13. Kano, Y. et al. (2005) Endocrinology 146:5332.
14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-2/BMP-4 Antibody (BAF355)(2)

Publications using BAF355

• Tomoeda M, Yamada S, Shirai H, Ozawa Y, Yanagita M, Murakami S PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif. Biochem. Biophys. Res. Commun., 2008-04-11;371(2):191-6. 2008-04-11 [PMID: 18407830] (Western Blot, Human)
• Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A Baalc, a marker of mesoderm and muscle. Gene Expr. Patterns, 2005-04-01;5(4):463-73. 2005-04-01 [PMID: 15749074] (IHC, Mouse)

Bioinformatics

• Gene Symbol: BMP2
• Uniprot:
  • P12643()