BMP-2/BMP-4 Antibody [Unconjugated]

BMP‑2/BMP‑4 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human BMP‑2/BMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF355) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BMP-2/BMP-4 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
  Gln283-Arg396
  Accession # P12643
• Specificity: Detects BMP-2 and BMP-4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BMP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-2/BMP-4 Antibody [Unconjugated]

• BDA2
• BMP-2
• BMP-2/BMP-4
• BMP2A
• BMP-2A
• BMP2ABone morphogenetic protein 2A
• bone morphogenetic protein 2

Background

Human BMP-2 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse and rat BMP-2 are 100% identical. BMP-2 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 114 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. One BMP type II and two BMP type I receptors have been identified. In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type Is can bind BMP-2 with high-affinity in the absence of BMP receptor type II. 

1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49
2. Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
3. Reddi, A.H. (1998) Nature Biotechnology 16: 247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-2/BMP-4 Antibody (AF355)(9)

Publications using AF355

• MM Menger, B Merscher, C Scheuer, BJ Braun, SC Herath, MF Rollmann, D Stenger, T Später, T Pohlemann, MD Menger, T Histing Amlodipine accelerates bone healing in a stable closed femoral fracture model in mice European cells & materials, 2021-05-24;41(0):592-602. 2021-05-24 [PMID: 34027631] (Western Blot, Mouse)
• MG Kim, CL Kim, YS Kim, JW Jang, GM Lee Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7 Scientific Reports, 2021-02-09;11(1):3378. 2021-02-09 [PMID: 33564092] (ICC, Hamster)
• Li Zhang, Chunfang Hao, Yansheng Wu, Yuying Zhu, Yulin Ren, Zhongsheng Tong <p>Microcalcification and BMP-2 in breast cancer: correlation with clinicopathological features and outcomes</p> OncoTargets and Therapy 2019-04-03 [PMID: 30936719]
• Liene Smane, Mara Pilmane Evaluation of the presence of MMP-2, TIMP-2, BMP2/4, and TGF beta 3 in the facial tissue of children with cleft lip and palate Acta medica Lituanica 2018-09-14 [PMID: 30210242]
• Péter Csomor, Balázs Liktor, Bálint Liktor, Zoltán Szekanecz, István Sziklai, Tamás Karosi Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci Acta Oto-Laryngologica 2012-06-01 [PMID: 22385409]
• Jessica B. Lennington, Sara Pope, Anna E. Goodheart, Linda Drozdowicz, Stephen B. Daniels, John D. Salamone et al. Midbrain Dopamine Neurons Associated with Reward Processing Innervate the Neurogenic Subventricular Zone The Journal of Neuroscience 2011-09-14 [PMID: 21917791]
• Ehrhart NP, Eurell JA, Constable PD, Gaddy D, Nicholas RW The effect of host tissue irradiation on large-segment allograft incorporation. Clin. Orthop. Relat. Res., 2005-06-01;0(435):43-51. 2005-06-01 [PMID: 15930920] (IHC-P, Canine)
• Katri Koli, Frank Wempe, Anja Sterner-Kock, Anna Kantola, Martina Komor, Wolf-K. Hofmann et al. Disruption of LTBP-4 function reduces TGF-beta activation and enhances BMP-4 signaling in the lung The Journal of Cell Biology 2004-10-11 [PMID: 15466481]
• Knosp WM, Scott V, Bachinger HP, Stadler HS HOXA13 regulates the expression of bone morphogenetic proteins 2 and 7 to control distal limb morphogenesis. Development, 2004-09-01;131(18):4581-92. 2004-09-01 [PMID: 15342482] (IHC, Mouse)

Bioinformatics

• Gene Symbol: BMP2
• Uniprot:
  • P12643()