Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, pH 7.4
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 0.5 ug/ml
This BMP2 antibody is useful for Immunohistochemistry-Paraffin and Western Blot, where a band is seen ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-19751 in the following applications:
Bone morphogenic protein 2 is required for effective healing of fractures in bone. It is necessary for the expression of transcription factors that regulate osteogenesis and differentiation of progenitor cells into mature osteoblasts. Embryologically, BMP2 is important in mesenchymal maturation to fetal valves. The same mechanism of maturation is also utilized by tumor molecular machinery once mesenchymal stem cells are recruited to tumor microenvironments, leading to many recent attempts to generate BMP inhibitors in the treatment of ovarian and colon cancer. The activity of BMP2 is antagonized by the activity of Bambi and Crim1. BMP2 is an interesting target for distinguishing the mechanism by which normal and necessary maturation processes are mutated in tumorigenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for BMP-2 Antibody (NBP1-19751). (Showing 1 - 2 of 2 FAQs).
I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.
We onced ordered an anti BMP2 antibody (NBP1-19751) from your company, and have gotten great WB results. Your product has been included in our manuscript. Now reviewers are asking about the cross activity of this antibody with other BMPs, such as BMP6 or BMP9. Did you ever check the cross-react of the BMP2 antibody?
Unfortunately, we have not tested this antibody against other/recombinant BMPs so far but I can assure you that it will not be expected to react with any other BMPs. NBP1-19751 was generated against an immunogen peptide from AA250-350 of BMP2 protein [Swiss-Prot# P12643], and this sequence does not show any sequence similarity/homology with any other BMPs.