Bmf Antibody - BSA Free

Western Blot: Bmf Antibody [NBP1-76666] - Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76666] - Immunofluorescence of Bmf in Human Kidney cells with Bmf antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bmf Antibody - BSA Free Summary

• Immunogen: Antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf. The immunogen is located within the last 50 amino acids of Bmf. Amino Acid Squence: ALNGEENRNGAGPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMF
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 2.5-5 ug/mL
• Application Notes: A band at approximately 25 kDa can be detected in Western Blot.
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Bmf Peptide (NBP1-76666PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bmf Antibody - BSA Free

• Bcl2 modifying factor
• bcl-2-modifying factor
• FLJ00065

Background

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMF
• Uniprot:
  • NP_277038(Human)