| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72311 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76666-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99460-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97262-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22130-50ul | |||
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody [NBP1-76658] - Analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0005.jpg)
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs incubated w/ M-CSF (30 ng/ml) & RANKL (40 ng/ml) for 40 h, washed thoroughly, & incubated further w/ LPS (50 ng/ml) in presence of M-CSF (30 ng/ml) for 48 h. (a) Cells transfected w/ 30 nM of miR-29b mimic or con mimic in presence of M-CSF (30 ng/ml) for 6 h. Total RNA analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, & HRK. Expression levels w/ con mimic treatment set at 1. Cell lysates subjected to WB analysis w/ antibodies against BMF, PUMA, BAK1, BIM, HRK, & BCL-2. Antibodies against beta -actin used for normalization. (b) w/out transfection, total RNA analyzed by qPCR to quantify the expression of BMF, & cell lysates subjected to WB analysis w/ anti-BMF Ab. (c) Total RNA & tissue lysate of tibiae from LPS-treated or vehicle-treated (V, PBS) mice analyzed by qPCR to quantify the expression of BMF & subjected to WB analysis w/ antibodies against BMF. (d) Cells thoroughly washed, transfected w/ 30 nM of anti-miR-29b or con inh, & stimulated w/ LPS (50 ng/ml) in presence of M-CSF. After 48 h, total RNA analyzed by qPCR to quantify BMF expression. Expression levels w/ con inh treatment set at 1.0. Cell lysates subjected to WB analysis w/ anti-BMF & anti-BCL-2 Ab. (e) Cells thoroughly washed, transfected w/ 50 nM of scRNA or siBMF, stimulated w/ LPS (50 ng/ml) in presence of M-CSF for 48 h, & analyzed to measure TRAP-positive MNCs & annexin V-positive cells. siRNA-mediated silencing of BMF confirmed by RT-PCR & qPCR. The Ct values of genes widely distributed between 17.33 & 30.92. ∗p Red: Bmf Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-2711202419432229.gif)
![Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76658] - Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-271120242022971.jpg)
![Western Blot: BMF Antibody (6F3) [NBP2-46578] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Western-Blot-NBP2-46578-img0006.jpg "Western Blot: BMF Antibody (6F3) [NBP2-46578] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .")
![Immunohistochemistry: BMF Antibody (6F3) [NBP2-46578] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Immunohistochemistry-NBP2-46578-img0005.jpg)
![Immunohistochemistry: BMF Antibody (6F3) [NBP2-46578] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min).](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Immunohistochemistry-NBP2-46578-img0001.jpg)
![Immunohistochemistry: BMF Antibody (6F3) [NBP2-46578] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Immunohistochemistry-NBP2-46578-img0002.jpg)
![Immunohistochemistry: BMF Antibody (6F3) [NBP2-46578] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Immunohistochemistry-NBP2-46578-img0003.jpg)
![Immunohistochemistry: BMF Antibody (6F3) [NBP2-46578] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BMF-Antibody-6F3-Immunohistochemistry-NBP2-46578-img0004.jpg)
![Western Blot: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Western-Blot-NBP2-72311-img0001.jpg "Western Blot: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .")
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0005.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min).](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0002.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0003.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0004.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0006.jpg)
![Flow Cytometry: Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Data of overexpressed EE-tagged Bmf in 293T cells, fixed with 1% PFA, permeabilized with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. NBP2-80091)](http://images.novusbio.com/fullsize/Bmf-Antibody-17A9-BSA-Free-Flow-Cytometry-NBP2-80091-img0004.jpg "Flow Cytometry: Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Data of overexpressed EE-tagged Bmf in 293T cells, fixed with 1% PFA, permeabilized with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. NBP2-80091)")
![<b>Genetic Strategies Validation. </b>Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Using Bmf (mouse/rat), mAb (17A9) (Prod. No. NBP2-80091) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.](http://images.novusbio.com/fullsize/Bmf-Antibody-17A9-BSA-Free-NBP2-80091-img0001.jpg)
![Immunoprecipitation: Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No. NBP2-80091).](http://images.novusbio.com/fullsize/Bmf-Antibody-17A9-BSA-Free-Immunoprecipitation-NBP2-80091-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP1-84660] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84660-img0008.jpg "Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP1-84660] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Bmf Antibody [NBP1-84660] - Staining of human bone marrow shows moderate cytoplasmic positivity in megakaryocytes.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunohistochemistry-Paraffin-NBP1-84660-img0007.jpg)
![Western Blot: Bmf Antibody [NBP1-76666] - Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76666-img0002.jpg "Western Blot: Bmf Antibody [NBP1-76666] - Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76666] - Immunofluorescence of Bmf in Human Kidney cells with Bmf antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76666_rabbit-polyclonal-bmf-antibody-271120241929385.jpg)
![Western Blot: Bmf Antibody [NBP2-99460] - Anti-Bmf rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP2-99460-img0003.jpg "Western Blot: Bmf Antibody [NBP2-99460] - Anti-Bmf rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa")
![Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP2-99460] - Immunofluorescence staining of Bmf in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Bmf polyclonal antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99460-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP2-97262] - Immunofluorescence staining of Bmf in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Bmf polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97262-img0001.jpg "Immunocytochemistry/Immunofluorescence: Bmf Antibody [NBP2-97262] - Immunofluorescence staining of Bmf in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Bmf polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.")
![Immunohistochemistry-Paraffin: Bmf Antibody [NBP2-97262] - Immunochemical staining of human Bmf in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Bmf-Antibody-Immunohistochemistry-Paraffin-NBP2-97262-img0002.jpg)
![Western Blot: Bmf Antibody (SR1375) [NBP3-22130] - Western blot analysis using NBP3-22130 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22130_rabbit-bmf-mab-sr1375-57202316494222.jpg "Western Blot: Bmf Antibody (SR1375) [NBP3-22130] - Western blot analysis using NBP3-22130 in Ramos cell lysate.")
![Western Blot: Bmf Antibody (JE33-57) [NBP3-32085] - Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (NBP3-32085) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: Mouse pancreas tissue lysate Lane 6: Rat pancreas tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32085) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32085_rabbit-bmf-mab-je33-57-278202416331222.jpg "Western Blot: Bmf Antibody (JE33-57) [NBP3-32085] - Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (NBP3-32085) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: Mouse pancreas tissue lysate Lane 6: Rat pancreas tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32085) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![ELISA: Human Bmf ELISA Kit (Colorimetric) [NBP2-69975] - Samples were spiked with high concentrations of Human Bmf and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69975_human-bmf-elisa-kit-colorimetric-13220241620412.jpg "ELISA: Human Bmf ELISA Kit (Colorimetric) [NBP2-69975] - Samples were spiked with high concentrations of Human Bmf and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Bmf ELISA Kit (Colorimetric) [NBP2-69975] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69975_human-bmf-elisa-kit-colorimetric-432024973694.jpg)