Bmf Antibody - BSA Free

Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.

Western Blot: Bmf Antibody [NBP1-76658] - Analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 ug/ml.

Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs incubated w/ M-CSF (30 ng/ml) & RANKL (40 ng/ml) for 40 h, washed thoroughly, & incubated further w/ LPS (50 ng/ml) in presence of M-CSF (30 ng/ml) for 48 h. (a) Cells transfected w/ 30 nM of miR-29b mimic or con mimic in presence of M-CSF (30 ng/ml) for 6 h. Total RNA analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, & HRK. Expression levels w/ con mimic treatment set at 1. Cell lysates subjected to WB analysis w/ antibodies against BMF, PUMA, BAK1, BIM, HRK, & BCL-2. Antibodies against beta -actin used for normalization. (b) w/out transfection, total RNA analyzed by qPCR to quantify the expression of BMF, & cell lysates subjected to WB analysis w/ anti-BMF Ab. (c) Total RNA & tissue lysate of tibiae from LPS-treated or vehicle-treated (V, PBS) mice analyzed by qPCR to quantify the expression of BMF & subjected to WB analysis w/ antibodies against BMF. (d) Cells thoroughly washed, transfected w/ 30 nM of anti-miR-29b or con inh, & stimulated w/ LPS (50 ng/ml) in presence of M-CSF. After 48 h, total RNA analyzed by qPCR to quantify BMF expression. Expression levels w/ con inh treatment set at 1.0. Cell lysates subjected to WB analysis w/ anti-BMF & anti-BCL-2 Ab. (e) Cells thoroughly washed, transfected w/ 50 nM of scRNA or siBMF, stimulated w/ LPS (50 ng/ml) in presence of M-CSF for 48 h, & analyzed to measure TRAP-positive MNCs & annexin V-positive cells. siRNA-mediated silencing of BMF confirmed by RT-PCR & qPCR. The Ct values of genes widely distributed between 17.33 & 30.92. ∗p Red: Bmf Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76658] - Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bmf Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bmf Antibody - BSA Free (NBP1-76658) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Bmf Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf. The immunogen is located within the first 50 amino acids of Bmf. Amino Acid Squence: EPSQCVEELEDDV
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMF
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-10 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Bmf Peptide (NBP1-76658PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bmf Antibody - BSA Free

• Bcl2 modifying factor
• bcl-2-modifying factor
• FLJ00065

Background

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bmf Antibody (NBP1-76658)(2)

Publications using NBP1-76658

• Popescu B, Stahlhut C, Tarver TC et al. Allosteric SHP2 inhibition increases apoptotic dependency on BCL2 and synergizes with venetoclax in FLT3- and KIT-mutant AML Cell reports. Medicine 2023-11-21 [PMID: 37992684]
• Sul O. J, Rajasekaran M, et al. MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation. Oxid Med Cell Longev 2019-05-17 [PMID: 31093317] (WB, Mouse)

Bioinformatics

• Gene Symbol: BMF
• Uniprot:
  • NP_277038(Human)