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SUR1 Antibody (S289-16)

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Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.
Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 ...read more
Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor ...read more
Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Lane 1: Molecular Weight Ladder. ...read more
Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S289-16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-59320AF405
Alexa Fluor 488
NBP2-59320AF488
Alexa Fluor 647
NBP2-59320AF647
Alexa Fluor 700
NBP2-59320AF700
Biotin
NBP2-59320B
DyLight 350
NBP2-59320UV
DyLight 405
NBP2-59320V
DyLight 488
NBP2-59320G
DyLight 550
NBP2-59320R
DyLight 650
NBP2-59320C
DyLight 680
NBP2-59320FR
DyLight 755
NBP2-59320IR
FITC
NBP2-59320F
HRP
NBP2-59320H
Janelia Fluor 549
NBP2-59320JF549
Janelia Fluor 646
NBP2-59320JF646
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SUR1 Antibody (S289-16) Summary

Immunogen
Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
Localization
Membrane
Specificity
Detects 160kDa. Does not cross-react with SUR2B.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCC8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-59320 in the following applications:
Publications
Read Publication using
NBP2-59320 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS pH7.4, 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for SUR1 Antibody (S289-16)

  • ABC36
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 8
  • HHF1Sulfonylurea receptor 1
  • HI
  • PHHIHRINS
  • sulfonylurea receptor (hyperinsulinemia)
  • SUR1MRP8
  • SURATP-binding cassette transporter sub-family C member 8
  • TNDM2ATP-binding cassette sub-family C member 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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(24-1) [Unconjugated] ...
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CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(11 Reviews)
(41 Publications)
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Applications: WB, IHC, IP
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(31A1067) - (Pro and ...
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Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
(1 Review)
(153 Publications)
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
MRP1 Antibody
(IU2H10)
NB400-156
Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)
(15 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, Dual ISH-IHC

Publications for SUR1 Antibody (NBP2-59320)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Reviews for SUR1 Antibody (NBP2-59320) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-59320:
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Images Ratings Applications Species Date Details
Western Blot SUR1 NBP2-59320
Enlarge 		4
reviewed by:
Luiz Barella
 WB Mouse 02/25/2019
View

Summary

ApplicationWestern Blot
Sample TestedMouse pancreatic islets
SpeciesMouse
Lot1205

Comments

CommentsWestern Blot analysis of mouse pancreatic islets using Mouse Anti-SUR1 S289-16. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10 ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16 hours at 4 degrees celsius. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT.
Immunohistochemistry-Paraffin SUR1 NBP2-59320
Enlarge 		4
reviewed by:
Anonymous
 IHC-P Human 07/07/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tissue Sections - Pancreas
SpeciesHuman
Lot1205

Comments

CommentsSUR1 was detected in immersion fixed paraffin-embedded sections of normal human pancreas using citrate pH6 pretreatment and incubating at 1:50 dilution for 30 minutes, with LabVision Quanto Detection.

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FAQs for SUR1 Antibody (NBP2-59320) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-59320AF405
Alexa Fluor 488 NBP2-59320AF488
Alexa Fluor 647 NBP2-59320AF647
Alexa Fluor 700 NBP2-59320AF700
Biotin NBP2-59320B
DyLight 350 NBP2-59320UV
DyLight 405 NBP2-59320V
DyLight 488 NBP2-59320G
DyLight 550 NBP2-59320R
DyLight 650 NBP2-59320C
DyLight 680 NBP2-59320FR
DyLight 755 NBP2-59320IR
FITC NBP2-59320F
HRP NBP2-59320H
Janelia Fluor 549 NBP2-59320JF549
Janelia Fluor 646 NBP2-59320JF646
Learn about Custom Antibody Labeling

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Recent Reviews

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Western Blot SUR1 NBP2-59320
Enlarge
 Luiz Barella
02/25/2019
Application: WB
Species: Mouse
read this review
Immunohistochemistry-Paraffin SUR1 NBP2-59320
Enlarge
 Anonymous
07/07/2017
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol ABCC8