SUR1 Antibody (S289-16)

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.

Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.

Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC
• Clone: S289-16
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SUR1 Antibody (S289-16) Summary

• Immunogen: Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
• Localization: Membrane
• Specificity: Detects 160kDa. Does not cross-react with SUR2B.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for SUR1 Antibody (S289-16)

• ABC36
• ABCC8
• ATP-binding cassette, sub-family C (CFTR/MRP), member 8
• HHF1Sulfonylurea receptor 1
• HI
• HRINS
• PHHIHRINS
• sulfonylurea receptor (hyperinsulinemia)
• SUR
• SUR1
• SUR1MRP8
• SURATP-binding cassette transporter sub-family C member 8
• TNDM2ATP-binding cassette sub-family C member 8

Background

SUR1 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternative splicing of this gene has been observed; however, the transcript variants have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUR1 Antibody (NBP2-59320)(4)

Publications using NBP2-59320

• Zhou J, Zhang Z, Yang Y et al. Deletion of serine racemase reverses neuronal insulin signaling inhibition by amyloid-beta oligomers Journal of neurochemistry 2022-07-15 [PMID: 35839294]
• Yeh S, Hsu P, Yeh T et al. Capping Protein Regulator and Myosin 1 Linker 3 (CARMIL3) as a Molecular Signature of Ischemic Neurons in the DWI-T2 Mismatch Areas After Stroke Frontiers in Molecular Neuroscience 2021-12-16 [PMID: 34975397] (IF/IHC, Rat)
• Tsymbalyuk O, Gerzanich V, Mumtaz A et al. SUR1, newly expressed in astrocytes, mediates neuropathic pain in a mouse model of peripheral nerve injury Molecular pain 2021-03-31 [PMID: 33788643] (IF/IHC, Mouse)
• Gerzanich V, Stokum J, Ivanova S et al. SUR1, TRPM4 and KIR6.2 - role in hemorrhagic progression of contusion. J. Neurotrauma 2018-08-30 [PMID: 30160201] (IF/IHC, Human)

Reviews for SUR1 Antibody (NBP2-59320) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Mouse 02/25/2019

Summary

• Application: Western Blot
• Sample Tested: Mouse pancreatic islets
• Species: Mouse
• Lot: 1205

Comments

• Comments: Western Blot analysis of mouse pancreatic islets using Mouse Anti-SUR1 S289-16. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10 ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16 hours at 4 degrees celsius. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT.

reviewed by: Kristi Bailey IHC-P Human 07/07/2017

Summary

• Application: IHC-P
• Sample Tested: Human Tissue Sections - Pancreas
• Species: Human
• Lot: 1205

Comments

• Comments: SUR1 was detected in immersion fixed paraffin-embedded sections of normal human pancreas using citrate pH6 pretreatment and incubating at 1:50 dilution for 30 minutes, with LabVision Quanto Detection.

Bioinformatics

• Gene Symbol: ABCC8