beta-III Tubulin Antibody (2E9)

Images

 
Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of Tubulin Beta 3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysates.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - IF Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse ...read more
Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Simple Western lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF
Clone
2E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-III Tubulin Antibody (2E9) Summary

Immunogen
Full length recombinant human Tubulin Beta 3 expressed in E. coli. [UniProt# Q13509].
Marker
Neuronal Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:500
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Application Notes
This Tubulin Beta 3 (2E9) antibody is useful in Western Blot, Immunocytochemistry/Immunofluorescence, Flow cytometry and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42568 in the following applications:

Publications
Read Publication using NBP1-42568.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for beta-III Tubulin Antibody (2E9)

  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Tubulin Beta 3 is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of Tubulin Beta 3 is almost exclusively neuron-specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-III Tubulin Antibody (NBP1-42568)(1)

We have publications tested in 1 application: IHC.


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Review for beta-III Tubulin Antibody (NBP1-42568) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-42568:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry beta-III Tubulin NBP1-42568
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reviewed by:
Anony- mous
ICC Human 06/20/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-III Tubulin Antibody (NBP1-42568) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Recent Reviews

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5
1
4
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2
0
1
0

Anony- mous
06/20/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TUBB3
Entrez
Uniprot