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beta II Tubulin A Antibody

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Western Blot: beta II Tubulin A Antibody [NBP2-15581] - beta II Tubulin A Antibody detects beta II Tubulin A protein by western blot analysis. A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract. D. 30 ug Raw 264.7 whole cell lysate/extract. E. 30 ug C2Cl2 whole cell lysate/extract. 10 % SDS-PAGE. beta II Tubulin A Antibody dilution: 1:2000
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - beta II Tubulin A Antibody detects beta II Tubulin A protein by western blot analysis. A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell ...read more
Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody [NBP2-15581] - HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: TUBB2A protein stained by TUBB2A antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581] - Mouse duodenum Paraffin-embedded mouse duodenum. beta Tubulin 2 antibody dilution: 1:500.
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - Sample (30 ug of whole cell lysate) A: Drosophila lysate 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - A. 50 ug rat brain lysate/extract 10 % SDS-PAGE beta Tubulin 2 antibody dilution: 1:2000
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - beta II Tubulin A Antibody detects beta II Tubulin A protein by western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell ...read more
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - beta II Tubulin A Antibody detects beta II Tubulin A protein by western blot analysis. A. 30 ug U87-MG whole cell lysate/extract. B. 30 ug SK-N-SH whole cell ...read more
Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, DrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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beta II Tubulin A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB2A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
beta II Tubulin A Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for beta II Tubulin A Antibody

  • dJ40E16.7
  • TUBB
  • TUBB2
  • TUBB2B
  • tubulin beta-2A chain
  • tubulin, beta 2
  • tubulin, beta 2A
  • tubulin, beta polypeptide 2
  • tubulin, beta polypeptide

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta ‑III Tubulin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(17 Reviews)
(152 Publications)
Species: All-Multi
Applications: WB, Simple Western, IHC, ICC
GAPDH Antibody
(1D4)
NB300-221
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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(327 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
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Actin Gamma 1
Antibody
NB600-533
Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (http://neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
(6 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
Recombinant Human
DNMBP GST (N-Term) Pr ...
H00023268-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
FUBI/MNSF beta/FAU
Antibody
NBP1-55090
Western Blot: FUBI/MNSF beta/FAU Antibody [NBP1-55090] - MCF7, Antibody Dilution: 1.0 ug/ml FAU is supported by BioGPS gene expression data to be expressed in MCF7.
Immunohistochemistry: FUBI/MNSF beta/FAU Antibody [NBP1-55090] - Human Adult liver Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 secProtocol located in Reviews and Data.
Western Blot: FUBI/MNSF beta/FAU Antibody [NBP1-55090] - 721_B cell lysate, concentration 0.2-1 ug/ml.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
beta-Actin
Antibody (AC-15)
NB600-501
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
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(443 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
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beta I Tubulin
Antibody (2A1A9)
NBP2-52577
Western Blot: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis using beta I Tubulin mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate.
Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Vimentin Antibody
NB300-223
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.
Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.
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(45 Publications)
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Plexin A1 Antibody
[Unconjugated]
AF4309
Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(1 Publication)
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Applications: WB, IHC, ICC
Tubulin Beta 4
Antibody (OTI3F1)
NBP2-00812
Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 4 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin Beta 4.
Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Human tonsil using anti-Tubulin Beta 4 mouse monoclonal antibody.
(2 Publications)
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
RPL10 Antibody
NBP1-84037
Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: RPL10 Antibody [NBP1-84037] - Analysis in human cell line HeLa.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NOV/CCN3 Antibody
AF1640
NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Publications)
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
Recombinant Human
TUBB6 GST (N-Term) Pr ...
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12.5% SDS-PAGE Stained with Coomassie Blue.
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MHC Class I
Antibody (OX18)
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
(32 Publications)
Species: Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
IGF-I/IGF-1
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
(5 Reviews)
(141 Publications)
Species: Hu
Tubulin Beta 2C
Antibody (ST52-04)
NBP2-67520
Western Blot: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of beta II Tubulin on different lysates using anti-beta II Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse kidney
Immunocytochemistry/Immunofluorescence: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Staining beta II Tubulin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Tubulin Beta 2C Antibody (ST52-04) [NBP2-67520] - Analysis of paraffin-embedded mouse brain tissue using anti-beta II Tubulin antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
RPL13 Antibody
NBP1-57476
Western Blot: RPL13 Antibody [NBP1-57476] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: RPL13 Antibody [NBP1-57476] - Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar mostly type I cells Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: RPL13 Antibody [NBP1-57476] - Jurkat cell lysate, concentration 0.0625ug/ml.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
GCHFR Antibody
(846011) [Unconjugate
MAB7918
Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu
Applications: WB, Simple Western
Enolase 1 Antibody
(8G8)
H00002023-M01
Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
(7 Publications)
Species: Hu, Fe
Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP
beta II Tubulin A
Antibody
NBP2-15581
beta II Tubulin A Antibody
Species: Hu, Mu, Rt, Dr
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Control Lysate(s)

beta II Tubulin A Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TUBB2A
Uniprot