ERCC1 Antibody (8F1)

Images

 
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.

Product Details

Summary
Reactivity Hu, Rt, ChHaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
8F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ERCC1 Antibody (8F1) Summary

Immunogen
Full length recombinant human ERCC1. [UniProt# P07992]
Localization
Nuclear.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ERCC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Antibody can be used in Immunocytochemistry/Immunoflourescence as reported in the literature (PMID: 21961533)Western Blot was reported in scientific literature.
Positive Control
Tonsil Lysate (NB820-59272)
ERCC1 Lysate (NBL1-10318)
ERCC1 Lysate (NBP2-64887)
Reviewed Applications
Read 1 Review rated 5
using
NB500-704 in the following applications:

Publications
Read Publications using
NB500-704 in the following applications:

  • IHC
    10 publications
  • 2 publications
  • WB
    3 publications

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ERCC1 Antibody (8F1)

  • COFS4
  • DNA excision repair protein ERCC-1
  • ERCC1
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence)
  • RAD10
  • UV20

Background

ERCC1, also known as RAD10, COFS4 and UV20, is a gene that functions in the nucleotide excision repair pathway. Specifically, ERCC1 is required for the repair of DNA lesions induced by UV light or formed by electrophilic compounds. ERCC1 is a heterodimer composed of ERCC1 and XPF/ERRC4 and is located in the nucleus. Mutations in ERCC1 result in cerebrooculofacio skeletal syndrome, as well as carcinogenesis. Recent research on DNA repair suggests that DNA damage may drive accelerated bone aging (PMID: 23281008), and that defaults in DNA repair mechanisms are prevalent in many lung cancers (PMID: 23276504).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P

Publications for ERCC1 Antibody (NB500-704)(15)

We have publications tested in 2 confirmed species: Human, Chinese Hamster.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(10)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(14)
Chinese Hamster
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB500-704 Applications Species
Bepler G, Zinner RG, Moon J et al. A phase 2 cooperative group adjuvant trial using a biomarker-based decision algorithm in patients with stage I non-small cell lung cancer (SWOG-0720, NCT00792701). Cancer 2014 Apr 18 [PMID: 24752945] (IHC-P, Human) IHC-P Human
Bepler G, Williams C, Schell MJ et al. Randomized international phase III trial of ERCC1 and RRM1 expression-based chemotherapy versus gemcitabine/carboplatin in advanced non-small-cell lung cancer. J Clin Oncol. 2013 Jul 1 [PMID: 23690416] (IHC-P, Human) IHC-P Human
Ozcan MF, Dizdar O, Dincer N et al. Low ERCC1 expression is associated with prolonged survival in patients with bladder cancer receiving platinum-based neoadjuvant chemotherapy Urol Oncol 2012 Aug 2 [PMID: 22863869] (IHC, Human) IHC Human
Sodja E, Knez L, Kern I et al. Impact of ERCC1 expression on treatment outcome in small-cell lung cancer patients treated with platinum-based chemotherapy Eur J Cancer 2012 Jul 11 [PMID: 22795264] (IHC, Human) IHC Human
Su CH, Chu WC, Lan KH et al. Gemcitabine causes telomere attrition by stabilizing TRF3 Eur J Cancer 2012 Jun 13 [PMID: 22704123] (WB, Human) WB Human
Soltermann A, Kilgus-Hawelski S, Behnke S et al. Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: Evaluation of antibodies 8F1 and D-10. Journal of Clinical Bioinformatics;1(1):25. 2011 Sep 30. [PMID: 21961533]
Besse B, Massard C, Haddad V et al. ERCC1 influence on the incidence of brain metastases in patients with non-squamous NSCLC treated with adjuvant cisplatin-based chemotherapy. Ann Oncol. 2011 Mar [PMID: 20801905] (IHC, Human) IHC Human
Lee S, Park YH, Kim KH et al. Thymidine synthase, thymidine phosphorylase, and excision repair cross-complementation group 1 expression as predictive markers of capecitabine plus cisplatin chemotherapy as first-line treatment for patients with advanced oesophageal squamous cell carcinoma. Br J Cancer. 2010 Sep 7 [PMID: 20700125] (IHC, Human) IHC Human
Shao YY, Kuo KT, Hu FC et al. Predictive and prognostic values of tau and ERCC1 in advanced breast cancer patients treated with paclitaxel and cisplatin. Jpn J Clin Oncol. 2010 Apr [PMID: 20085902] (IHC, Human) IHC Human
Bhaqwat NR, Roginskaya VY, Acquafondata MB et al. Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue. Cancer Res. 2009 Sep 1 [PMID: 19723666] (IHC, WB, Human) IHC, WB Human
Show All 15 Publications.

Review for ERCC1 Antibody (NB500-704) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-704:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin ERCC1 NB500-704
Enlarge
reviewed by:
Anonymous
IHC-P Human 12/14/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedNon-small cell lung cancer
SpeciesHuman
CommentsAnti-ERCC1 ab clone 8F1 was tested on both cytologic and histologic material and demonstrated robust staining of the nuclear surface on both Ventana Benchmark and Leica Bond automated IHC platforms. No significant background was observed.
Pub Med IDPMID:21961533
FileView PDF

Details

Fixation DetailsSample Preparation: Formalin fixed and paraffin embedded

Comments

CommentsAnti-ERCC1 ab clone 8F1 was tested on both cytologic and histologic material and demonstrated robust staining of the nuclear surface on both Ventana Benchmark and Leica Bond automated IHC platforms. No significant background was observed.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERCC1 Antibody (NB500-704) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB500-704UV
DyLight 405 Labeled NB500-704V
DyLight 488 Labeled NB500-704G
DyLight 680 Labeled NB500-704FR
DyLight 755 Labeled NB500-704IR

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Blogs on ERCC1.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.

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Recent Reviews

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1
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1
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Anonymous
12/14/2011
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ERCC1
Entrez
Uniprot