Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody [NBP2-92640] - Analysis of A-549 cells using Beta Hydroxysteroid Dehydrogenase .

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Beta Hydroxysteroid Dehydrogenase, using Beta Hydroxysteroid Dehydrogenase Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 0.5s.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1). MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD3B2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

• 3 beta-HSD type II
• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
• 3-beta-HSD II
• delta 5-delta 4-isomerase type II
• HSD3B
• HSDB
• HSDB3B
• hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase
• progesterone reductase
• SDR11E2
• short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase

Background

The protein encoded by the Beta Hydroxysteroid Dehydrogenases gene is a bifunctional enzyme that catalyzes the oxidative conversion ofdelta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in thebiosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads.Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternativelyspliced transcript variants have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD3B2