HSD3B1 Antibody (FDO66Q)

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Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.

Product Details

Summary
Reactivity Hu, Rt, Bb, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
FDO66Q
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

HSD3B1 Antibody (FDO66Q) Summary

Immunogen
JEG Choriocarcinoma cells
Localization
Membrane
Marker
Trophoblast-associated Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD3B1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
Application Notes
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Positive Control
HSD3B1 Lysate (NBL1-11732)
Publications
Read Publications using
NB110-78644 in the following applications:

Reactivity Notes

Human, baboon, bovine, rat and marmoset.

Alternate Names for HSD3B1 Antibody (FDO66Q)

  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
  • 3-beta-HSD I
  • 3BETAHSD
  • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
  • 3BH
  • delta-5-3-ketosteroid isomerase
  • EC 1.1.1
  • HSD3B
  • HSDB3
  • HSDB3A
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
  • I
  • progesterone reductase
  • SDR11E1
  • short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
  • steroid Delta-isomerase
  • Trophoblast antigen FDO161G

Background

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSD3B1 Antibody (NB110-78644)(4)

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Product General Protocols

View specific protocols for HSD3B1 Antibody (NB110-78644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSD3B1 Antibody (NB110-78644) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-78644AF405
Alexa Fluor 488 Labeled NB110-78644AF488
Alexa Fluor 647 Labeled NB110-78644AF647
Alexa Fluor 700 Labeled NB110-78644AF700
Biotin Labeled NB110-78644B
DyLight 350 Labeled NB110-78644UV
DyLight 405 Labeled NB110-78644V
DyLight 488 Labeled NB110-78644G
DyLight 550 Labeled NB110-78644R
DyLight 650 Labeled NB110-78644C
DyLight 680 Labeled NB110-78644FR
DyLight 755 Labeled NB110-78644IR
FITC Labeled NB110-78644F
HRP Labeled NB110-78644H
PerCP Labeled NB110-78644PCP

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Bioinformatics

Gene Symbol HSD3B1

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