HSD3B1 Antibody (FDO66Q)

Images

 
Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Gt, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
FDO66Q
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HSD3B1 Antibody (FDO66Q) Summary

Immunogen
JEG Choriocarcinoma cells
Localization
Membrane
Marker
Trophoblast-associated Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD3B1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
Application Notes
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Control
HSD3B1 Overexpression Lysate
Publications
Read Publications using
NB110-78644 in the following applications:

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 31158446). Porcine reactivity reported in scientific literature (Bidne et al).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSD3B1 Antibody (FDO66Q)

  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
  • 3-beta-HSD I
  • 3BETAHSD
  • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
  • 3BH
  • delta-5-3-ketosteroid isomerase
  • EC 1.1.1
  • HSD3B
  • HSDB3
  • HSDB3A
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
  • I
  • progesterone reductase
  • SDR11E1
  • short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
  • steroid Delta-isomerase
  • Trophoblast antigen FDO161G

Background

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSD3B1 Antibody (NB110-78644)(7)

We have publications tested in 4 confirmed species: Rat, Bovine, Goat, Porcine.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
WB
(4)
All Applications
Filter By Species
Rat
(2)
Bovine
(1)
Goat
(1)
Porcine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-78644 Applications Species
Bidne KL. Investigating the ovarian response to endotoxemia. Thesis Jul 9 1905 (WB, Porcine) WB Porcine
An SY, Zhang GM, Liu ZF, et al MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A Gene 2019 May 31 [PMID: 31158446] (WB, Goat) WB Goat
Zhang R, Kikuchi AT, Nakao T et al. Identification and functional characterization of microRNAs in rat Leydig cells during development from the progenitor to the adult stage Mol. Cell. Endocrinol. May 23 2019 [PMID: 31129276] (WB, Rat) WB Rat
Palaniappan M, Menon KM. Luteinizing Hormone/Human Chorionic Gonadotropin-Mediated Activation of mTORC1 Signaling Is Required for Androgen Synthesis by Theca-Interstitial Cells Mol Endocrinol 2012 Oct [PMID: 22827930] (WB, Rat) WB Rat
Irving-Rodgers HF, Krupa M, Rodgers RJ. Cholesterol side-chain cleavage cytochrome P450 and 3beta-hydroxysteroid dehydrogenase expression and the concentrations of steroid hormones in the follicular fluids of different phenotypes of healthy and atretic bovine ovarian follicles. Biol Reprod;69(6):2022-8. 2003 Dec. [PMID: 12930727]
Irving-Rodgers HF, Bathgate RA, Ivell R, Domagalski R, Rodgers RJ. Dynamic changes in the expression of relaxin-like factor (INSL3), cholesterol side-chain cleavage cytochrome p450, and 3beta-hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and atresia. Biol Reprod;66(4):934-43. 2002 Apr. [PMID: 11906911] (IHC-Fr, Bovine) IHC-Fr Bovine
Mueller, UW et al. Isolation of fetal trophoblast cells from peripheral blood of pregnant women. Lancet;336(8709):197-200. 1990 Jul 28. [PMID: 1973769] (ICC/IF) ICC/IF

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Product General Protocols

View specific protocols for HSD3B1 Antibody (NB110-78644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSD3B1 Antibody (NB110-78644) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-78644AF350
Alexa Fluor 405 NB110-78644AF405
Alexa Fluor 488 NB110-78644AF488
Alexa Fluor 532 NB110-78644AF532
Alexa Fluor 594 NB110-78644AF594
Alexa Fluor 647 NB110-78644AF647
Alexa Fluor 700 NB110-78644AF700
Alexa Fluor 750 NB110-78644AF750
Biotin NB110-78644B
DyLight 350 NB110-78644UV
DyLight 405 NB110-78644V
DyLight 488 NB110-78644G
DyLight 550 NB110-78644R
DyLight 594 NB110-78644DL594
DyLight 650 NB110-78644C
DyLight 680 NB110-78644FR
DyLight 755 NB110-78644IR
FITC NB110-78644F
HRP NB110-78644H
Janelia Fluor 549 NB110-78644JF549
Janelia Fluor 646 NB110-78644JF646

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Bioinformatics

Gene Symbol HSD3B1