Aldo-keto Reductase 1C3/AKR1C3 Antibody

Images

 
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 ...read more
Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunoprecipitation: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 ug of AKR1C3 antibody [C2C3], C-term. Western blot analysis was ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Aldo-keto Reductase 1C3/AKR1C3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKR1C3. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKR1C3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 40% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Aldo-keto Reductase 1C3/AKR1C3 Antibody

  • 17-beta-hydroxysteroid dehydrogenase type 5
  • 3-alpha-HSD type 2
  • 3-alpha-hydroxysteroid dehydrogenase type 2
  • AKR1C3
  • Aldo-keto Reductase 1C3
  • aldo-keto reductase family 1 member C3
  • aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
  • AldoketoReductase 1C3
  • Chlordecone reductase homolog HAKRb
  • DD3
  • DD-3
  • DD317-beta-HSD 5
  • DDH1
  • DDX
  • Dihydrodiol dehydrogenase 3
  • Dihydrodiol dehydrogenase type I
  • dihydrodiol dehydrogenase X
  • EC 1.1.1
  • EC 1.1.1.112,3-alpha-HSD type II, brain
  • EC 1.1.1.188
  • EC 1.1.1.213
  • EC 1.1.1.63
  • EC 1.1.1.64
  • EC 1.3.1.20
  • HA1753
  • HAKRB
  • HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
  • HAKRe
  • HSD17B5
  • hydroxysteroid (17-beta) dehydrogenase 5
  • Indanol dehydrogenase
  • KIAA0119hluPGFS
  • PGFS
  • Prostaglandin F synthase
  • Testosterone 17-beta-dehydrogenase 5
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AKR1C3