Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011)

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Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
799011
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 3/CHST3
Glu39-Thr479
Accession # Q7LGC8
Specificity
Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CHST3
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011)

  • C6ST1
  • C6ST-1
  • C6ST1HSD
  • C6STEC 2.8.2.17
  • carbohydrate (chondroitin 6) sulfotransferase 3
  • Carbohydrate Sulfotransferase 3
  • Chondroitin 6-O-sulfotransferase 1
  • Chondroitin 6-sulfotransferase
  • CHST3
  • EC 2.8.2
  • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
  • GST-0

Background

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).
  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
  5. Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.
  6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHST3
Entrez
Uniprot