NGFI-B alpha/Nur77/NR4A1 Antibody

Images

 
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. ...read more
Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Antibody tested in Jurkat cells at a 1:50 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to aa 251-266 of human Nak1 (NP_775180).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR4A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation 1:20-1:1000
Application Notes
Chromatin Immunoprecipitation: See Lee et al (2009). ICC/IF: See Chintharlapalli et al (2005) Immunoprecipitation: See Ferlini et al (2009). Use in Immunohistochemistry reported in scientific literature (PMID: 31115481).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-56745 in the following applications:

Publications
Read Publications using
NB100-56745 in the following applications:

  • 3 publications
  • 2 publications
  • IF
    1 publication
  • IHC
    2 publications
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

  • Early response protein NAK1
  • GFRP1ST-59
  • growth factor-inducible nuclear protein N10
  • HMR
  • HMRNP10
  • hormone receptor
  • MGC9485
  • N10
  • NAK1
  • NAK-1
  • NGFIB alpha
  • NGFI-B alpha
  • NGFIB
  • NR4A1
  • Nuclear hormone receptor NUR/77
  • nuclear receptor subfamily 4 group A member 1
  • nuclear receptor subfamily 4, group A, member 1
  • Nur77
  • Orphan nuclear receptor HMR
  • Orphan nuclear receptor TR3
  • steroid receptor TR3
  • Testicular receptor 3
  • TR3 orphan receptor
  • TR3

Background

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC/IF, IF, IHC, WB.


Filter By Application
ChIP
(3)
ICC/IF
(2)
IF
(1)
IHC
(2)
WB
(9)
All Applications
Filter By Species
Human
(5)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-56745 Applications Species
Li XC, Yin XJ, Hong W et al. The orphan nuclear receptor NUR77 promotes trophoblast invasion at early pregnancy through paracrine placental growth factor J. Mol. Med. Jul 16 2019 [PMID: 31312859]
Qin H, Gao F, Wang Y et al. Nur77 promotes cigarette smoke-induced autophagic cell death by increasing the dissociation of Bcl2 from Beclin-1 Int. J. Mol. Med. May 8 2019 [PMID: 31115481] (IHC, Mouse) IHC Mouse
Imura T, kobayashi Y, Suzutani k et al. Differential expression of a stress-regulated gene Nr4a2 characterizes early- and late-born hippocampal granule cells. Hippocampus. Oct 26 2018 [PMID: 30365199] (Mouse) Mouse
Hedrick Erik, Lee Syng-Ook, Safe Stephen et al. The nuclear orphan receptor NR4A1 regulates beta 1-integrin expression in pancreatic and colon cancer cells and can be targeted by NR4A1 antagonists. Molecular Carcinogenesis 2017 May 9 [PMID: 28418095] (ChIP, Human) ChIP Human
Mohankumar K, Li X, Sung N et al. BIS-INDOLE DERIVED NUCLEAR RECEPTOR 4A1 (NR4A1, Nur77) LIGANDS AS INHIBITORS OF ENDOMETRIOSIS Endocrinology Feb 26 2020 [PMID: 32099996]
Shin JH, Lee G, Jeong MG et al. Transcriptional coactivator with PDZ-binding motif suppresses the expression of steroidogenic enzymes by nuclear receptor 4 A1 in Leydig cells FASEB J. Feb 17 2020 [PMID: 32067268] (ChIP, Mouse) ChIP Mouse
Carpenter MD, Hu Q, Bond AM et al. Nr4a1 suppresses cocaine-induced behavior via epigenetic regulation of homeostatic target genes Nat Commun Jan 24 2020 [PMID: 31980629] (ChIP, Mouse) ChIP Mouse
Zhang Y, Federation A J et al. Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity. J Clin Invest Jan 11 2018 [PMID: 30277475] (IHC, Mouse) IHC Mouse
Li H, Zhao P, Xu Q et al. The autism-related gene SNRPN regulates cortical and spine development via controlling nuclear receptor Nr4a1 Sci Rep 2016 Jul 19 [PMID: 27430727] (WB) WB
Show All 20 Publications.

Reviews for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-56745:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
Enlarge
5
reviewed by:
Anonymous
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedG361 human melanoma cell line
SpeciesHuman
LotE-2
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/19/2018
View

Summary

ApplicationWestern Blot
Sample Tested4T1 cells whole cell lysate,Hepa 1-6 mouse hepatoma cell line
SpeciesMouse
LotE-2
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
Enlarge
5
reviewed by:
Anonymous
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedMDA MB 231 cells,A549 cells
SpeciesHuman
LotE-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745). (Showing 1 - 1 of 1 FAQs).

  1. We have a customer who would like to use NB100-56745 in ChIP experiments. However, they would like to see the data that suggests this antibody could be used in ChIP, which we do not have. Could you pass along that data? Thanks!
    • Product NB100-56745 is a new product added to our catalog from our recent acquisition with Imgenex. They had verified the use of ChIP with this product from the following publication: p21 expression is induced by activation of nuclear nerve growth factor-induced Ba (Nur77) in pancreatic cancer cells. Lee S-O, S Chintharlapalli, S Liu, S Papineni, SD Cho, K Yoon, S Safe. Mol Cancer Res 7:1169-1178 (2009). IF: Fig 2 (Panc1 cells) WB: Figs 3C and 4B (Panc1 or L3.6pl cells transfected with scrambled or Nur77 siRNA); Figs 6A, 6D (Panc1 cells) ChIP: Fig 5 (Panc1 cells) Note: Nur77 was detected in the nuclear, but not the cytoplasmic fraction of Panc1 cells by WB (Fig 6D). Note: The Nur77 IMG-528 antibody was Nur77 siRNA transfected validated by western blot. Nur77 siRNA, but not scrambled siRNA reduced the intensity of the WB signal in PANC1 and L3.6pi cells (Figs 3C, 4B).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56745AF350
Alexa Fluor 405 NB100-56745AF405
Alexa Fluor 488 NB100-56745AF488
Alexa Fluor 532 NB100-56745AF532
Alexa Fluor 594 NB100-56745AF594
Alexa Fluor 647 NB100-56745AF647
Alexa Fluor 700 NB100-56745AF700
Alexa Fluor 750 NB100-56745AF750
Biotin NB100-56745B
DyLight 350 NB100-56745UV
DyLight 405 NB100-56745V
DyLight 488 NB100-56745G
DyLight 550 NB100-56745R
DyLight 594 NB100-56745DL594
DyLight 650 NB100-56745C
DyLight 680 NB100-56745FR
DyLight 755 NB100-56745IR
FITC NB100-56745F
HRP NB100-56745H
Janelia Fluor 549 NB100-56745JF549
Janelia Fluor 646 NB100-56745JF646

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
04/04/2020
Application: WB
Species: Human

Anonymous
12/19/2018
Application: WB
Species: Mouse

Anonymous
10/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol NR4A1
Entrez
Uniprot