NGFI-B alpha/Nur77/NR4A1 Antibody

Images

 
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels ...read more
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of NGFI-B alpha/Nur77/NR4A1 antibody (NB100-56745). The staining was ...read more
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NGFI-B ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

Immunogen
Partial synthetic peptide made to an internal portion of the human NGFI-B alpha protein (between amino acids 200-300) [UniProt P22736].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR4A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
Application Notes
Chromatin Immunoprecipitation: See Lee et al (2009). ICC/IF: See Chintharlapalli et al (2005) Immunoprecipitation: See Ferlini et al (2009). Use in Immunohistochemistry reported in scientific literature (PMID: 31115481).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-56745 in the following applications:

Publications
Read Publications using
NB100-56745 in the following applications:

  • 4 publications
  • 2 publications
  • IF
    1 publication
  • IHC
    3 publications
  • IP
    1 publication
  • WB
    11 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

  • Early response protein NAK1
  • GFRP1ST-59
  • growth factor-inducible nuclear protein N10
  • HMR
  • HMRNP10
  • hormone receptor
  • MGC9485
  • N10
  • NAK1
  • NAK-1
  • NGFIB alpha
  • NGFI-B alpha
  • NGFIB
  • NR4A1
  • Nuclear hormone receptor NUR/77
  • nuclear receptor subfamily 4 group A member 1
  • nuclear receptor subfamily 4, group A, member 1
  • Nur77
  • Orphan nuclear receptor HMR
  • Orphan nuclear receptor TR3
  • steroid receptor TR3
  • Testicular receptor 3
  • TR3 orphan receptor
  • TR3

Background

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Supplier Logo

Publications for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, ICC/IF, IF, IHC, IP, WB.


Filter By Application
ChIP
(4)
ICC/IF
(2)
IF
(1)
IHC
(3)
IP
(1)
WB
(11)
All Applications
Filter By Species
Human
(7)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-56745 Applications Species
Jiang C, He J, Xu S et al. NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer Cell death discovery Feb 2 2022 [PMID: 35110542] (ChIP, IHC, Human) ChIP, IHC Human
Bruno NE, Nwachukwu JC, Hughes DC Et al. Activation of Crtc2/Creb1 in skeletal muscle enhances weight loss during intermittent fasting FASEB journal : official publication of the Federation of American Societies for Experimental Biology Dec 1 2021 [PMID: 34748223] (WB, Mouse) WB Mouse
Guo H, Golczer G, Wittner BS Et al. NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer Molecular cell Oct 7 2021 [PMID: 34624217] (IP, Human) IP Human
Ding R, Sun X, Yi B et al. Nur77 Attenuates Inflammasome Activation by Inhibiting Caspase-1 Expression in Pulmonary Vascular Endothelial Cells American journal of respiratory cell and molecular biology May 10 2021 [PMID: 33971110] (WB) WB
Li XC, Yin XJ, Hong W et al. The orphan nuclear receptor NUR77 promotes trophoblast invasion at early pregnancy through paracrine placental growth factor J. Mol. Med. Jul 16 2019 [PMID: 31312859]
Zhang Y, Federation A J et al. Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity. J Clin Invest Jan 11 2018 [PMID: 30277475] (IHC, Mouse) IHC Mouse
Hedrick Erik, Lee Syng-Ook, Safe Stephen et al. The nuclear orphan receptor NR4A1 regulates beta 1-integrin expression in pancreatic and colon cancer cells and can be targeted by NR4A1 antagonists. Molecular Carcinogenesis 2017 May 9 [PMID: 28418095] (ChIP, Human) ChIP Human
Mohankumar K, Li X, Sung N et al. BIS-INDOLE DERIVED NUCLEAR RECEPTOR 4A1 (NR4A1, Nur77) LIGANDS AS INHIBITORS OF ENDOMETRIOSIS Endocrinology Feb 26 2020 [PMID: 32099996]
Shin JH, Lee G, Jeong MG et al. Transcriptional coactivator with PDZ-binding motif suppresses the expression of steroidogenic enzymes by nuclear receptor 4 A1 in Leydig cells FASEB J. Feb 17 2020 [PMID: 32067268] (ChIP, Mouse) ChIP Mouse
Show All 24 Publications.

Reviews for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-56745:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
Enlarge
5
reviewed by:
Verified Customer
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedG361 human melanoma cell line
SpeciesHuman
LotE-2
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
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4
reviewed by:
Verified Customer
WB Mouse 12/19/2018
View

Summary

ApplicationWestern Blot
Sample Tested4T1 cells whole cell lysate,Hepa 1-6 mouse hepatoma cell line
SpeciesMouse
LotE-2
Western Blot NGFI-B alpha/Nur77/NR4A1 NB100-56745
Enlarge
5
reviewed by:
Verified Customer
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedA549 cells,MDA MB 231 cells
SpeciesHuman
LotE-2

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745). (Showing 1 - 1 of 1 FAQs).

  1. We have a customer who would like to use NB100-56745 in ChIP experiments. However, they would like to see the data that suggests this antibody could be used in ChIP, which we do not have. Could you pass along that data? Thanks!
    • Product NB100-56745 is a new product added to our catalog from our recent acquisition with Imgenex. They had verified the use of ChIP with this product from the following publication: p21 expression is induced by activation of nuclear nerve growth factor-induced Ba (Nur77) in pancreatic cancer cells. Lee S-O, S Chintharlapalli, S Liu, S Papineni, SD Cho, K Yoon, S Safe. Mol Cancer Res 7:1169-1178 (2009). IF: Fig 2 (Panc1 cells) WB: Figs 3C and 4B (Panc1 or L3.6pl cells transfected with scrambled or Nur77 siRNA); Figs 6A, 6D (Panc1 cells) ChIP: Fig 5 (Panc1 cells) Note: Nur77 was detected in the nuclear, but not the cytoplasmic fraction of Panc1 cells by WB (Fig 6D). Note: The Nur77 IMG-528 antibody was Nur77 siRNA transfected validated by western blot. Nur77 siRNA, but not scrambled siRNA reduced the intensity of the WB signal in PANC1 and L3.6pi cells (Figs 3C, 4B).

Secondary Antibodies

 

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Blogs on NGFI-B alpha/Nur77/NR4A1.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
04/04/2020
Application: WB
Species: Human

Verified Customer
12/19/2018
Application: WB
Species: Mouse

Verified Customer
10/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol NR4A1
Entrez
Uniprot