NGFI-B alpha/Nur77/NR4A1 Antibody

Images

 
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. ...read more
Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - NUR77 antibody was tested in Jurkat cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to aa 251-266 of human Nak1 (NP_775180).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR4A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:20-1:1000
Application Notes
Chromatin Immunoprecipitation: See Lee et al (2009) Immunocytochemistry/Immunofluorescence: See Chintharlapalli et al (2005) Immunoprecipitation: See Ferlini et al (2009)
Publications
Read Publications using
NB100-56745 in the following applications:

  • 2 publications
  • IF
    1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

  • Early response protein NAK1
  • GFRP1ST-59
  • growth factor-inducible nuclear protein N10
  • HMR
  • HMRNP10
  • hormone receptor
  • MGC9485
  • N10
  • NAK1
  • NAK-1
  • NGFIB alpha
  • NGFI-B alpha
  • NGFIB
  • NR4A1
  • Nuclear hormone receptor NUR/77
  • nuclear receptor subfamily 4 group A member 1
  • nuclear receptor subfamily 4, group A, member 1
  • Nur77
  • Orphan nuclear receptor HMR
  • Orphan nuclear receptor TR3
  • steroid receptor TR3
  • Testicular receptor 3
  • TR3 orphan receptor
  • TR3

Background

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF, WB.


Filter By Application
ICC/IF
(2)
IF
(1)
WB
(9)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56745 Applications Species
Li H, Zhao P, Xu Q et al. The autism-related gene SNRPN regulates cortical and spine development via controlling nuclear receptor Nr4a1 Sci Rep 2016 Jul 19 [PMID: 27430727] (WB) WB
Li X, Lee SO, Safe S. Structure-dependent activation of NR4A2 (Nurr1) by 1,1-bis(3'-indolyl)-1-(aromatic)methane analogs in pancreatic cancer cells. Biochem Pharmacol. 2012 May 15 [PMID: 22405837] (WB)

Details:
WB (Panc1 and Panc28 human pancreatric cancer cell lines): Fig 2C.
WB
Yoon K, Lee SO, Cho SD et al. Activation of nuclear TR3 (NR4A1) by a diindolylmethane analog induces apoptosis and proapoptotic genes in pancreatic cancer cells and tumors. Carcinogenesis. 2011 Jun [PMID: 21362629] (WB, Human)

Details:
WB (human pancreatic cancer cell lines): Figs 1,2.
WB Human
Lee SO, Abdelrahim M, Yoon K et al. Inactivation of the orphan nuclear receptor TR3/Nur77 inhibits pancreatic cancer cell and tumor growth. Cancer Res. 2010 Sep 1 [PMID: 20660371]
Cho SD, Lee SO, Chintharlapalli S et al. Activation of nerve growth factor-induced B alpha by methylene-substituted diindolylmethanes in bladder cancer cells induces apoptosis and inhibits tumor growth. Mol Pharmacol. 2010 Mar [PMID: 20023005] (ICC/IF, Human)

Details:
IF/ICC, Fig 1: human bladder cancer cells.
ICC/IF Human
Levin VA, Panchabhai SC, Shen L et al. Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines. J Proteome Res. 2010 Jan [PMID: 19894763] (WB, Human)

Details:
The IMG-528 Nak1/Nur77 IMG-528 pAb was used in a denatured protein array and for WB (human glioblastomas): Tables 1, 2.
WB Human
Ferlini C, Cicchillitti L, Raspaglio G et al. Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77. Cancer Res. 2009 Sep 1 [PMID: 19671798] (WB)

Details:
A2780 human ovarian cell line: WB (Figs 4A, 4B, 5A-top panel), IP/WB (Fig 5A-bottom panel). Note: A2780 cell lysate was IP'd with Bcl-2 (Figs 4A, 4B) or Tubulin (Fig 5A) antibodies T, followed by WB with the IMG-528 Nur77 antibody. The results showed tha
WB
Lee SO, Chintharlapalli S, Liu S et al. p21 expression is induced by activation of nuclear nerve growth factor-induced Balpha (Nur77) in pancreatic cancer cells. Mol Cancer Res. 2009 Jul [PMID: 19584258] (WB, IF)

Details:
IF: Fig 2 (Panc1 cells) WB: Figs 3C and 4B (Panc1 or L3.6pl cells transfected with scrambled or Nur77 siRNA); Figs 6A, 6D (Panc1 cells) ChIP: Fig 5 (Panc1 cells) Note: Nur77 was detected in the nuclear, but not the cytoplasmic fraction of Panc1 cells by W
WB, IF
Inamoto T, Papineni S, Chintharlapalli S et al. 1,1-Bis(3'-indolyl)-1-(p-chlorophenyl)methane activates the orphan nuclear receptor Nurr1 and inhibits bladder cancer growth. Mol Cancer Ther. 2008 Dec [PMID: 19074857] (WB, Human)

Details:
WB (ten human bladder cancer cell lines): Fig 1A.
WB Human
Tao R, Hancock WW. Resistance of Foxp3+ regulatory T cells to Nur77-induced apoptosis promotes allograft survival. PLoS One. 2008 May 28 [PMID: 18509529] (WB)

Details:
WB (primary mouse T cells): Fig 1C. T cell lysate was IP'd with an HDAC7 antibody, followed by WB with the IMG-528 Nur77 antibody. The results showed that Nur77 co-IP'd with HDAC7 and that Nur77 formed a complex with HDAC7.
WB
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745). (Showing 1 - 1 of 1 FAQs).

  1. We have a customer who would like to use NB100-56745 in ChIP experiments. However, they would like to see the data that suggests this antibody could be used in ChIP, which we do not have. Could you pass along that data? Thanks!
    • Product NB100-56745 is a new product added to our catalog from our recent acquisition with Imgenex. They had verified the use of ChIP with this product from the following publication: p21 expression is induced by activation of nuclear nerve growth factor-induced Ba (Nur77) in pancreatic cancer cells. Lee S-O, S Chintharlapalli, S Liu, S Papineni, SD Cho, K Yoon, S Safe. Mol Cancer Res 7:1169-1178 (2009). IF: Fig 2 (Panc1 cells) WB: Figs 3C and 4B (Panc1 or L3.6pl cells transfected with scrambled or Nur77 siRNA); Figs 6A, 6D (Panc1 cells) ChIP: Fig 5 (Panc1 cells) Note: Nur77 was detected in the nuclear, but not the cytoplasmic fraction of Panc1 cells by WB (Fig 6D). Note: The Nur77 IMG-528 antibody was Nur77 siRNA transfected validated by western blot. Nur77 siRNA, but not scrambled siRNA reduced the intensity of the WB signal in PANC1 and L3.6pi cells (Figs 3C, 4B).

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Bioinformatics

Gene Symbol NR4A1
Entrez
Uniprot