Western Blot: ATM Antibody (5C2) [NB100-220] - HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM protein stained by ATM antibody [5C2] diluted at 1:200. Blue: Hoechst ...read more
Western Blot: ATM Antibody (5C2) [NB100-220] - Detection of human ATM protein using ATM 5C2 monoclonal antibody in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2).
IHC usage reported in scientific literature (PMID: 21095582).
370 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Protein G purified
Alternate Names for ATM Antibody (5C2)
ataxia telangiectasia mutated (includes complementation groups A, C and D)
ataxia telangiectasia mutatedATD
serine-protein kinase ATM
TEL1, telomere maintenance 1, homolog
ATM-5C2 recognizes full-length ATM, a 370 kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post.
53BP1 - DNA damage is no fun The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri... Read full blog post.
ATM and DSB Repair in Cancer Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to... Read full blog post.
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