ATM Antibody (5C2)


Western Blot: ATM Antibody (5C2) [NB100-220] - HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM protein stained by ATM antibody [5C2] diluted at 1:200. Blue: Hoechst more
Western Blot: ATM Antibody (5C2) [NB100-220] - Detection of human ATM protein using ATM 5C2 monoclonal antibody in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2).

Product Details

Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1.72 mg/ml

Order Details

ATM Antibody (5C2) Summary

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.
Protein G purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS [pH 7.4]
No Preservative
1.72 mg/ml
Protein G purified


  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Use in IHC was reported in the scientific literature (PMID: 21095582) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
370 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-220 in the following applications:

  • 19 publications
  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for ATM Antibody (5C2)

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutatedATD
  • ATC
  • ATDC
  • ATE
  • DKFZp781A0353
  • EC
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL


ATM-5C2 recognizes full-length ATM, a 370 kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-220)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-220 Applications Species
Aumailley L, Garand C, Dubois MJ et al. Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice. PLoS One 2015 [PMID:26447695] (ICC/IF, Mouse) ICC/IF Mouse
Mendez G, Cilli D, Berardinelli F et al. Cleavage of the BRCT tandem domains of nibrin by the 657del5 mutation affects the DNA damage response less than the Arg215Trp mutation IUBMB Life 2012 Oct [PMID:22941933]
Daniel JA, Pellegrini M, Lee BS et al. Loss of ATM kinase activity leads to embryonic lethality in mice J Cell Biol 2012 Aug 6 [PMID:22869595] (WB, Mouse) WB Mouse
Hou YY, Toh MT, Wang X. NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity. Cell Biochemistry and Function. 2011 Dec 9. [PMID:22161642]
Vogel R, Seyffert M, Strasser R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol 2012 Jan [PMID:22013059] (ICC/IF, Human) ICC/IF Human
Rankin AM, Sarkar S, Faller DV et al. Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells. J Cell Physiol 2012 Jun [PMID:21898405] (ICC/IF, Human) ICC/IF Human
Gapud EJ, Dorsett Y, Yin B et al. Ataxia telangiectasia mutated (Atm) and DNA-Pkcs kinases have overlapping activities during chromosomal signal joint formation. Proc Natl Acad Sci USA 2011 Feb [PMID:21245316] (ICC/IF, Human) ICC/IF Human
Cam H, Easton JB, High A et al. mTORC1 signaling under hypoxic conditions is controlled by ATM-dependent phosphorylation of HIF-1a. Mol Cell 2010 Nov [PMID:21095582] (ICC/IF, Human) ICC/IF Human
Craig AL, Holcakova J, Finlan LE et al. DeltaNp63 transcriptionally regulates ATM to control p53 Serine-15 phosphorylation. Mol Cancer 2010 Jul [PMID:20663147] (ICC/IF, Human) ICC/IF Human
Alimonti A, Nardella C, Chen Z et al. A novel type of cellular senescence that can be enhanced in mouse models and human tumor xenografts to suppress prostate tumorigenesis. J Clin Invest 2010 Mar [PMID:20197621]
Show All 24 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


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Blogs on ATM.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Gene Symbol ATM

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