ATM Antibody (5C2)

Images

 
Western Blot: ATM Antibody (5C2) [NB100-220] - HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM protein stained by ATM antibody [5C2] diluted at 1:200. Blue: Hoechst ...read more
Western Blot: ATM Antibody (5C2) [NB100-220] - Detection of human ATM protein using ATM 5C2 monoclonal antibody in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2).

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
5C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.43 mg/ml

Order Details

ATM Antibody (5C2) Summary

Immunogen
Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
IHC usage reported in scientific literature (PMID: 21095582).
Theoretical MW
370 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raji Lysate (NB820-59466)
Publications
Read Publications using
NB100-220 in the following applications:

  • 19 publications
  • WB
    3 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
2.43 mg/ml
Purity
Protein G purified

Alternate Names for ATM Antibody (5C2)

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutatedATD
  • ATC
  • ATDC
  • ATE
  • ATM
  • DKFZp781A0353
  • EC 2.7.11.1
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL

Background

ATM-5C2 recognizes full-length ATM, a 370 kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-220)(26)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(19)
WB
(3)
All Applications
Filter By Species
Human
(18)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-220 Applications Species
Barnard RA, Regan DP, Hansen RJ et al. Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease. J Pharmacol Exp Ther 2016 Aug [PMID: 27231155]
Aumailley L, Garand C, Dubois MJ et al. Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice. PLoS One 2015 [PMID: 26447695] (ICC/IF, Mouse) ICC/IF Mouse
Milan E, Perini T, Resnati M et al. A plastic SQSTM1/p62-dependent autophagic reserve maintains proteostasis and determines proteasome inhibitor susceptibility in multiple myeloma cells. Autophagy 2015 [PMID: 26043024] (WB) WB
Mendez G, Cilli D, Berardinelli F et al. Cleavage of the BRCT tandem domains of nibrin by the 657del5 mutation affects the DNA damage response less than the Arg215Trp mutation IUBMB Life 2012 Oct [PMID: 22941933]
Daniel JA, Pellegrini M, Lee BS et al. Loss of ATM kinase activity leads to embryonic lethality in mice J Cell Biol 2012 Aug 6 [PMID: 22869595] (WB, Mouse) WB Mouse
Hou YY, Toh MT, Wang X. NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity. Cell Biochemistry and Function. 2011 Dec 9. [PMID: 22161642]
Vogel R, Seyffert M, Strasser R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol 2012 Jan [PMID: 22013059] (ICC/IF, Human) ICC/IF Human
Rankin AM, Sarkar S, Faller DV et al. Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells. J Cell Physiol 2012 Jun [PMID: 21898405] (ICC/IF, Human) ICC/IF Human
Gapud EJ, Dorsett Y, Yin B et al. Ataxia telangiectasia mutated (Atm) and DNA-Pkcs kinases have overlapping activities during chromosomal signal joint formation. Proc Natl Acad Sci USA 2011 Feb [PMID: 21245316] (ICC/IF, Human) ICC/IF Human
Cam H, Easton JB, High A et al. mTORC1 signaling under hypoxic conditions is controlled by ATM-dependent phosphorylation of HIF-1a. Mol Cell 2010 Nov [PMID: 21095582] (ICC/IF, Human) ICC/IF Human
Show All 26 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-220) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ATM Antibody (NB100-220)

Discover related pathways, diseases and genes to ATM Antibody (NB100-220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for ATM Antibody (NB100-220)

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Research Areas for ATM Antibody (NB100-220)

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Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Bioinformatics

Gene Symbol ATM
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