Anti-ATM Antibodies: Novus Biologicals

NB100-309

ATM Antibody (2C1) - Azide and BSA Free

(3 Reviews)

(132 Publications)

100 ul

NB100-309

Western Blot: ATM Antibody (2C1) [NB100-309] - Analysis of ATM in U2OS sarcoma cells using ATM antibody (2C1) [NB100-309]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verifeid customer review.

Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

<b>Genetic Strategies Validation. </b>Western Blot: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:500.

<b>Genetic Strategies Validation. </b>ATM-Antibody-2C1-Western-Blot-NB100-309-img0019.jpg

Immunoprecipitation: ATM Antibody (2C1) [NB100-309] - MDA-MB-231 whole cell lysates were immunoprecipitated with 2 ug ATM Antibody (NB100-309), followed by Western blot (primary antibody: NB100-309 at 1:1000, 4C overnight. Western blot image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>nb100-309_mouse-monoclonal-atm-antibody-2c1-25520231514910.jpg

Western Blot: ATM Antibody (2C1) [NB100-309] - Western blot analysis of the extent of knockdown MRE11 & ATM, & the lack of effect of Mirin activation of ATM. The extent of shRNA-mediated knockdown of MRE11 & ATM are shown for (A) clones GFP-7F1 & GFP-6D1 & (B) clones EDS-7F2 & EDS-6J8. The extent of knockdown was determined by analyzing the intensity of the ATM & MRE11 bands relative to the intensity of the loading control GAPDH bands (see Table 1). (C) The effect of Mirin on activation of ATM was determined by analysis of phosphorylation of ATM in response to ionizing radiation. Cultures treated with DMSO alone, 20 μM Mirin, or knockdown of ATM were analyzed by western blot 30 min after exposure to 10 Gy of ionizing radiation. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26209132), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATM Antibody (2C1) [NB100-309] - Rac1 inhibition abolishes IR-induced activation of both ATM & ATR signaling. (A) MCF-7 cells were treated with/without 20-Gy IR in the presence or absence of 100 μM NSC23766 & incubated for 1 hour at 37°C before analysis. To assess the ATM kinase activity, ATM was immunoprecipitated from cell lysate by using anti-ATM antibody (2C1) & assayed for ATM activity by using p53 recombinant protein as substrate. To measure the Chk2 activity, Chk2 was immunoprecipitated from cell lysate by using B-4 anti-Chk2 antibody & assayed for Chk2 activity by using Cdc25C recombinant protein as substrate. As controls, ATM & Chk2 protein levels in the immunoprecipitates (IP-WB) as well as in cell lysates (WB) were assessed with immunoblotting. (B) ATR & Chk1 were immunoprecipitated from cell lysates by using N-19 anti-ATR & G-4 anti-Chk1 antibody, respectively. ATR activity was assayed by using p53 recombinant protein substrate, & Chk1 activity assayed by using Cdc25C recombinant protein substrate. As controls, ATR & Chk1 protein levels in the immunoprecipitates (IP-WB), as well as in cell lysates (WB) were assessed with immunoblotting. (C) MCF-7 cells were exposed to IR at the indicated doses in the presence or absence of NSC23766, incubated for 1 hour, & assessed for Chk1 & Chk2 activities. *Kinase assay does not contain Cdc25C substrate. (D) T47D & ZR-75-1 cells were exposed to 10-Gy IR in the presence or absence of 100 μM NSC23766, incubated for 1 hour, & analyzed for Chk1 & Chk2 activities. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATM Antibody (2C1) [NB100-309] - Inhibition of Rac1 by N17Rac1 mutant or Rac1 siRNA diminishes IR-induced G2/M checkpoint activation. (A) MCF-7 cells were infected with Ad.N17Rac1 or Ad.Control for 24 hours & exposed to 15-Gy IR. Left panel: the cells were analyzed for DNA content 24 hours after IR. The result depicts the percentage of cells with 4N-DNA content & is shown as mean ± SD of quadruplicate samples. *P < 0.001 (n = 4), significant difference from the irradiated Ad.Control-infected cells. Right panel: Inset: at 15 minutes after IR, the infected cells were analyzed for Rac1 activities (Rac1-GTP) & protein levels (total Rac1). Bar graph: mitotic cells in the cell samples were analyzed 2 hours after IR. The result depicts the percentage of mitotic cells & is shown as mean ± SD of triplicate samples. **P = 0.002 (n = 3), significant difference from the irradiated Ad.Control-infected cells. (B) Upper panel: MCF-7 cells transfected with Rac1 siRNA (Rac1) or control siRNA (Control) were incubated for the indicated times & analyzed for protein levels of Rac1 & Actin. Lower panel: After 2-day incubation, the siRNA-transfected cells were exposed to IR, incubated for 24 hours, & assessed for DNA content. Results depict the percentage of cells with 4N-DNA content & represent the mean ± SD of three separate experiments in duplicate samples. *P < 0.001 (n = 6), significant difference from the irradiated Control-siRNA transfected cells. (C) After 2-day incubation, siRNA-transfected cells were treated with/without 20-Gy IR, incubated for 1 hour, & analyzed for ATM, ATR, Chk1, & Chk2 activities. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell extract and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (NB100-309) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: ATM Antibody (2C1) [NB100-309] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (NB100-309) diluted at 1:1000.

Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-104

ATM Antibody - BSA Free

(2 Reviews)

(100 Publications)

0.1 ml

NB100-104

<b>Biological Strategies Validation. </b>ATM-Antibody-Immunocytochemistry-Immunofluorescence-NB100-104-img0018.jpg

Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HeLa cells with Dylight 550-conjugated ATM Antibody [NB100-104R] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

<b>Biological Strategies Validation. </b>ATM-Antibody-Western-Blot-NB100-104-img0017.jpg

Western Blot: ATM Antibody [NB100-104] - Detection of ATM in Raji whole cell lysate using [NB100-104]. Observed molecular weight ~300 kDa. Theoretical molecular weight 351 kDa.

Immunocytochemistry/Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton X-100. Then cells were incubated with [NB100-104] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of ATM Antibody [NB100-104PE]. An intracellular stain was performed on HeLa cells with ATM antibody [NB100-104PE] (blue) and a matched isotype control [NBP2-24893PE] (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.

Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HepG2 cells with PE-conjugated ATM antibody [NB100-104PE] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - ATM is activated by spermidine in GM00637 cells.GM00637 cells with or without KU55933 pretreatment (a,b), & GM05849 cells (c) were exposed to 50 μM spermidine or CCCP, followed by immunofluorescence analyses of total & p-ATM on Ser-1981. The scale bar is 10 μm. Ratios of cells expressing p-ATM Ser-1981 to cells expressing total ATM were presented (d). 20–45 cells/condition from three experiments were collected. Values are mean ± SD, *p < 0.05 vs. control. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep24700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATM Antibody [NB100-104] - Depletion of SMARCAL1 induces DNA damage & checkpoint activation in iSML1 iPSCs. (A,B) The iSML1 iPSCs were cultured for 7 & 14 days in the presence of doxycycline (DOX) to induce SMARCAL1 downregulation & then immunostained. The graphs (top) show quantification of the number of gamma -H2AX-positive cells (A) or ATM-pSer1981-positive cells (B). Representative images from triplicate experiments are shown (bottom). (C) Immunoblot detection of the indicated DDR proteins in iSML1 iPSCs after 14 days of continuous treatment with DOX. Lamin B1 was used as the loading control. Data are mean±s.e.m. from three independent experiments. *P≤0.05, **P≤0.01, ***P≤0.001 (two-way ANOVA test). ns, not significant. Scale bars: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31515241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ATM Antibody [NB100-104] - Analysis of double strand break repair in MCF-7 monolayer & mammosphere populations. (a) Cells were exposed to 4 Gy 60Co gamma -radiation & the relative degree of double-strand breakage (DSB) was determined by the comet assay under neutral conditions immediately after exposure & at the times indicated after exposure. (b) The 'comets' (n of about 100) were categorized according to the NIH LISTSERV (Comet Assay Interest Group web site) in which type 1 comets display the least DNA damage & type 5 the most. The error bars represent the mean ± standard error of the mean in both panels. The comets of the unirradiated cells are labeled Cont. (c) Expression of proteins involved in the NHEJ pathway of DSB repair in response to increasing doses of ionizing radiation. Lysates were prepared from unirradiated cells & from cells harvested one hour after exposure to 1 or 10-Gy 60Co gamma -radiation & analyzed by immunoblotting with antibodies against several DSB repair proteins. Phospho-ATM & phospho-DNA-PKcs refer to phosphorylation of these proteins at Ser1981 & Ser2056, respectively. Actin served as a loading control. Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2583), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Ma

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ma

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NB100-104AF405
Alexa Fluor 488	NB100-104AF488
Alexa Fluor 647	NB100-104AF647
Alexa Fluor 700	NB100-104AF700
Biotin	NB100-104B
DyLight 405	NB100-104V
DyLight 488	NB100-104G
DyLight 550	NB100-104R
DyLight 594	NB100-104DL594
DyLight 650	NB100-104C
FITC	NB100-104F
HRP	NB100-104H
PE	NB100-104PE

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-104SS

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NB100-306

ATM [p Ser1981] Antibody (10H11.E12) - BSA Free

(1 Review)

(30 Publications)

0.1 ml

NB100-306

Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Human lymphoblastoid cells stained with ATM [p Ser1981] Antibody (10H11.E12). ICC/IF image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>nb100-306_mouse-monoclonal-atm-p-ser1981-antibody-10h11-e12-2552023151493.jpg

MCELNs decreased the DNA damage of H9C2 cells after radiation. (A) Immunofluorescence staining of gamma -H2A.X (green) in H9C2 cells after 48 h of culture with indicated treatment. The nucleus were stained with DAPI (blue), scale bar: 10 μm. Western blot images (B) and quantitation (C) of gamma -H2A.X in H9C2 cells after 48 h of culture with indicated treatment. (D) Immunofluorescence staining of p-ATM (green) in H9C2 cells after 48 h of culture with indicated treatment. The nucleus were stained with DAPI (blue), scale bar: 10 μm. Western blot images (E) and quantitation (F) of p-ATM and ATM in H9C2 cells after 48 h of culture with indicated treatment. IR (–/+): 0/16 Gy X-ray; MCELNs (–/+): 0/10 μg/mL. All data were represented as means +/- SD (n = 3 independent experiments). The statistical significance was evaluated by one-way ANOVA followed by the Turkey's multiple comparisons test among groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35509278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

OOEP is required for ATM activation and RAD51 recruitment to DNA damage sites Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29955025), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca(-)

Species Glossary

Reactivity: Hu, Mu, Rt, Ca(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-306AF350
Alexa Fluor 405	NB100-306AF405
Alexa Fluor 488	NB100-306AF488
Alexa Fluor 532	NB100-306AF532
Alexa Fluor 594	NB100-306AF594
Alexa Fluor 647	NB100-306AF647
Alexa Fluor 700	NB100-306AF700
Alexa Fluor 750	NB100-306AF750
Alexa Fluor Plus 405	NB100-306AFP405
Alexa Fluor Plus 488	NB100-306AFP488
Alexa Fluor Plus 555	NB100-306AFP555
Alexa Fluor Plus 594	NB100-306AFP594
Alexa Fluor Plus 647	NB100-306AFP647
Alexa Fluor Plus 680	NB100-306AFP680
Allophycocyanin	NB100-306APC
Biotin	NB100-306B
CoraFluor 1	NB100-306CL1
DyLight 350	NB100-306UV
DyLight 405	NB100-306V
DyLight 488	NB100-306G
DyLight 550	NB100-306R
DyLight 594	NB100-306DL594
DyLight 650	NB100-306C
DyLight 680	NB100-306FR
DyLight 755	NB100-306IR
FITC	NB100-306F
HRP	NB100-306H
Janelia Fluor 525	NB100-306JF525
Janelia Fluor 549	NB100-306JF549
Janelia Fluor 646	NB100-306JF646
Janelia Fluor 669	NB100-306JF669
PE	NB100-306PE
PerCP	NB100-306PCP
mFluor Violet 450 SE	NB100-306MFV450
mFluor Violet 500 SE	NB100-306MFV500
mFluor Violet 610 SE	NB100-306MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-306SS
Azide and BSA Free	NBP2-80586

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AF1655

ATM [p Ser1981] Antibody

(1 Review)

(34 Publications)

100 μg

AF1655

HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

<b>Biological Strategies Validation. </b>Resveratrol activates ATM in transformed human cell lines.(a, b) Human HEK293T cells were treated with 0.01 mM ATM inhibitor (KU-55933) or vehicle (DMSO) for 1 hour, followed by incubation in media also containing 0.1 mM resveratrol (or DMSO), as indicated. After 30 minutes incubation with resveratrol, H2O2 (0.1 mM) or bleomycin (1 µg/ml) was added as indicated for 30 minutes before harvesting. Western blotting was performed with with antibodies directed against ATM, phospho-ATM Ser-1981, p53, and phospho-p53 Ser-15 as indicated. (c, d) Human HEK293T cells were treated with resveratrol, bleomycin, or both as in (a) and probed for gamma -H2AX foci by immunofluorescence. Cell images (291, 267, 216, and 260 cells, respectively) were analyzed for foci using Image J software, and the average number of foci per cell as well as the percentage of cells containing >5 foci were quantitated. (e, f) Human HCT116 cells were incubated with KU-55933, resveratrol, H2O2, and bleomycin as in (a, b) but additional phosphorylation targets were examined using antibodies directed against Smc1, phospho-Smc1 Ser-957, Kap1, phospho-Kap1 Ser-824, Nbs1, phospho-Nbs1 Ser-343, Chk2, and phospho-Chk2 Thr-68 as indicated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097969), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DEK is necessary for the activation of gamma H2AX after ionizing radiation.(a) MEFs were treated with 10 Gy of gamma ionizing radiation (IR) and stained for gamma H2AX. Representative cross sections from z-stack confocal images are shown. (b) Quantification of cells with >10 gamma H2AX foci in (A). At least 50 cells per time point were counted in each biological replicate, and all foci within the z-stack were quantified. DEK−/− MEFs were unable to activate gamma H2AX above baseline after irradiation. (paired t-test, n = 3, mean ± SEM). (c) Quantification of the average total number of gamma H2AX foci in each cell from (A). (paired t-test, n = 3, mean ± SEM) The number of foci per cell remained unchanged in DEK−/− MEFs after irradiation. (d) Western blot analysis of gamma H2AX in MEFs at 6 hs following irradiation with 10 Gy. (e) Western blot analysis of C33A human cancer cells infected with adenovirus 48 hr prior to receiving 10 Gy IR (see also Supplementary Fig. S1). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28317934), licensed under a CC-BY license. Not internally tested by R&D Systems.

TCL cell lines show intrinsic DNA damage and basal activation of DDR. Healthy T lymphocytes isolated from healthy donors and malignant T cell lines (OCI-Ly12, KARPAS-299, SUP-T1, HH, HD-MAR-2, and JURKAT) were subjected to immunofluorescence for the evaluation of gamma H2AX or 53BP1 foci (A,B). The percentage (%) of cells displaying foci and the number of foci/cell in the subsets of cells with detectable foci are reported in (A,B), respectively. Data are expressed as the mean ± SD of independent experiments. Asterisks indicate statistically significant differences between each cell line and healthy T lymphocytes (* p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant). Representative immunofluorescence images (C,D) of experiments described in (A,B), original microscope magnification 100×. SUP-T1 cells were exposed to increasing concentrations of CHOEP (IC50, 4× and 8×, as in [5]) or to 20 μM etoposide for 3 h. After harvesting, untreated and treated cell lysates were analyzed by Western blot using the indicated antibodies (E). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35409194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1655U-100UG
Alexa Fluor 405	AF1655V-100UG
Alexa Fluor 488	AF1655G-100UG
Alexa Fluor 532	AF1655X-100UG
Alexa Fluor 594	AF1655T-100UG
Alexa Fluor 647	AF1655R-100UG
Alexa Fluor 700	AF1655N-100UG
Alexa Fluor 750	AF1655S-100UG
Alexa Fluor Plus 405	AF1655AFP405-100UG
Alexa Fluor Plus 488	AF1655AFP488-100UG
Alexa Fluor Plus 555	AF1655AFP555-100UG
Alexa Fluor Plus 594	AF1655AFP594-100UG
Alexa Fluor Plus 647	AF1655AFP647-100UG
Alexa Fluor Plus 680	AF1655AFP680-100UG
Allophycocyanin	AF1655A
Biotin	AF1655B
CoraFluor 1	AF1655CL1
DyLight 350	AF1655D
DyLight 405	AF1655E
DyLight 488	AF1655K
DyLight 550	AF1655L
DyLight 594	AF1655M
DyLight 650	AF1655W
DyLight 680	AF1655Y
DyLight 755	AF1655Z
FITC	AF1655F
HRP	AF1655H
Janelia Fluor 525	AF1655JF525
Janelia Fluor 549	AF1655I
Janelia Fluor 646	AF1655J
Janelia Fluor 669	AF1655JF669
PE	AF1655P
PerCP	AF1655C
mFluor Violet 450 SE	AF1655MFV450
mFluor Violet 500 SE	AF1655MFV500
mFluor Violet 610 SE	AF1655MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1655-SP

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NB100-220

ATM Antibody (5C2) - Azide and BSA Free

(36 Publications)

0.1 ml

NB100-220

Western Blot: ATM Antibody (5C2) [NB100-220] - Absence of Atm does not relieve the selective pressure to inactivate p53 during chemical carcinogenesis.A. Representative images of 3MC-fibrosarcomas immunostained for Arf, p53 & p21 (see also Table 1). The upper & middle rows are representative of the large majority of fibrosarcomas developed in wild-type (upper) & Atm-null (middle) mice, which are consistent with a mutant p53 (i.e. strongly positive for p53 & negative for p21). The lower row is representative of the fibrosarcomas developed in Arf-null mice, which are consistent with a functional p53 (i.e. very weakly positive for p53 & positive for p21). B. Examples of cancer cell lines established from 3MC-fibrosarcomas (each line derives from an independent fibrosarcoma). The genotype of the mice where the 3MC-fibrosarcomas were generated is indicated, as well as, the status of p53 as determined by a nutlin-sensitivity assay (see Supplementary Figures S5 & S6). Cell lines were exposed to 10 Gy & protein extracts were obtained 1h & 24h after irradiation. The levels of the indicated proteins were determined by immunoblotting using beta -actin as loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19421407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: ATM Antibody (5C2) [NB100-220] - Limited role of Atm in p53-mediated tumor suppression & DNA damage response in chemically-induced fibrosarcomas.A. Mice of the indicated genotypes, wt (n = 15), p53-super (n = 16), Atm-null (n = 9), p53-super/Atm-null (n = 13) & Arf-null (n = 12), were injected intramuscularly with 3-methyl-cholanthrene (3MC) & tumour development was monitored. Kaplan-Meier tumour-free curves were obtained & statistical significant differences (logrank test) were found for wt vs. p53-super (p<0.005), Atm-null vs. p53-super/Atm-null (p<0.001) & Arf-null vs. wt (p<0.0001). No significant differences were found for wt vs. Atm-null (p = 0.11), or p53-super vs. p53-super/Atm-null (p = 0.18). B. Quantification of the persistent DNA damage response ( gamma H2AX), proliferation (Ki67), & apoptosis (activated caspase-3) in 3MC-fibrosarcomas generated in mice of the indicated genotypes. Quantifications are relative to the 3MC-fibrosarcomas in wild-type mice. Values correspond to the average & standard deviation (n = 5 per genotype). Examples of the immunostainings are shown in the panels at the right. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19421407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - BAG3P209L aggregation leads to co-aggregation of proteasomal substrates. a Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Scale bar = 5 μm. b Suppression of GFP-HttQ74 aggregation of cells expressing a control, FLAG-BAG3WT or FLAG-BAG3P209L. Western blot against indicated antibodies is shown. c Quantification of GFP-HttQ74 aggregation of experiments similar to b. Relative percentage of SDS-insoluble protein levels are shown. Data represents the mean & standard deviation of three independent experiments. d Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Left column BAG3, middle column ubiquitin & right column is the merge of BAG3 (green), ubiquitin (red), & DAPI (blue). Scale bar = 5 μm. e Fractionation of HEK293 cells expressing HSPB8, a control or BAG3WT or BAG3P209L, together with either Ub-R-GFP or GFP-ODC (ornithine decarboxylase). Western blot against GFP, FLAG (BAG3), Myc (HSPB8), & tubulin are shown. f Fractionation of HEK293 cells expressing HSPB8 & indicated BAG3 variants. Western blot using ubiquitin (FK2) & tubulin antibodies are shown. The same samples as in Fig. 5e have been used, loading control is therefore the same. Source data are provided as a Source data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30559338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-678

ATM Antibody - BSA Free

(2 Reviews)

(2 Publications)

100 ul

NB100-678

Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .

Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-678AF350
Alexa Fluor 405	NB100-678AF405
Alexa Fluor 488	NB100-678AF488
Alexa Fluor 532	NB100-678AF532
Alexa Fluor 594	NB100-678AF594
Alexa Fluor 647	NB100-678AF647
Alexa Fluor 700	NB100-678AF700
Alexa Fluor 750	NB100-678AF750
Alexa Fluor Plus 405	NB100-678AFP405
Alexa Fluor Plus 488	NB100-678AFP488
Alexa Fluor Plus 555	NB100-678AFP555
Alexa Fluor Plus 594	NB100-678AFP594
Alexa Fluor Plus 647	NB100-678AFP647
Alexa Fluor Plus 680	NB100-678AFP680
Allophycocyanin	NB100-678APC
Biotin	NB100-678B
CoraFluor 1	NB100-678CL1
DyLight 350	NB100-678UV
DyLight 405	NB100-678V
DyLight 488	NB100-678G
DyLight 550	NB100-678R
DyLight 594	NB100-678DL594
DyLight 650	NB100-678C
DyLight 680	NB100-678FR
DyLight 755	NB100-678IR
FITC	NB100-678F
HRP	NB100-678H
Janelia Fluor 525	NB100-678JF525
Janelia Fluor 549	NB100-678JF549
Janelia Fluor 646	NB100-678JF646
Janelia Fluor 669	NB100-678JF669
PE	NB100-678PE
PerCP	NB100-678PCP
mFluor Violet 450 SE	NB100-678MFV450
mFluor Violet 500 SE	NB100-678MFV500
mFluor Violet 610 SE	NB100-678MFV610

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NB100-271

ATM Antibody - BSA Free

(4 Publications)

100 ul

NB100-271

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-271AF350
Alexa Fluor 405	NB100-271AF405
Alexa Fluor 488	NB100-271AF488
Alexa Fluor 532	NB100-271AF532
Alexa Fluor 594	NB100-271AF594
Alexa Fluor 647	NB100-271AF647
Alexa Fluor 700	NB100-271AF700
Alexa Fluor 750	NB100-271AF750
Alexa Fluor Plus 405	NB100-271AFP405
Alexa Fluor Plus 488	NB100-271AFP488
Alexa Fluor Plus 555	NB100-271AFP555
Alexa Fluor Plus 594	NB100-271AFP594
Alexa Fluor Plus 647	NB100-271AFP647
Alexa Fluor Plus 680	NB100-271AFP680
Allophycocyanin	NB100-271APC
Biotin	NB100-271B
CoraFluor 1	NB100-271CL1
DyLight 350	NB100-271UV
DyLight 405	NB100-271V
DyLight 488	NB100-271G
DyLight 550	NB100-271R
DyLight 594	NB100-271DL594
DyLight 650	NB100-271C
DyLight 680	NB100-271FR
DyLight 755	NB100-271IR
FITC	NB100-271F
HRP	NB100-271H
Janelia Fluor 525	NB100-271JF525
Janelia Fluor 549	NB100-271JF549
Janelia Fluor 646	NB100-271JF646
Janelia Fluor 669	NB100-271JF669
PE	NB100-271PE
PerCP	NB100-271PCP
mFluor Violet 450 SE	NB100-271MFV450
mFluor Violet 500 SE	NB100-271MFV500
mFluor Violet 610 SE	NB100-271MFV610

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AF2290

ATM Antibody [Unconjugated]

100 μg

AF2290

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>ATM was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (untreated; positive staining) and HeLa human cervical epithelial carcinoma cell line treated with CIP (negative staining) using Sheep Anti-Human ATM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2290) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; <a class=

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="ATM was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (untreated; positive staining) and HeLa human cervical epithelial carcinoma cell line treated with CIP (negative staining) using Sheep Anti-Human ATM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2290) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

<b>Biological Strategies Validation. </b>ATM was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human ATM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2290) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2290U-100UG
Alexa Fluor 405	AF2290V-100UG
Alexa Fluor 488	AF2290G-100UG
Alexa Fluor 532	AF2290X-100UG
Alexa Fluor 594	AF2290T-100UG
Alexa Fluor 647	AF2290R-100UG
Alexa Fluor 700	AF2290N-100UG
Alexa Fluor 750	AF2290S-100UG
Alexa Fluor Plus 405	AF2290AFP405-100UG
Alexa Fluor Plus 488	AF2290AFP488-100UG
Alexa Fluor Plus 555	AF2290AFP555-100UG
Alexa Fluor Plus 594	AF2290AFP594-100UG
Alexa Fluor Plus 647	AF2290AFP647-100UG
Alexa Fluor Plus 680	AF2290AFP680-100UG
Allophycocyanin	AF2290A
Biotin	AF2290B
CoraFluor 1	AF2290CL1
DyLight 350	AF2290D
DyLight 405	AF2290E
DyLight 488	AF2290K
DyLight 550	AF2290L
DyLight 594	AF2290M
DyLight 650	AF2290W
DyLight 680	AF2290Y
DyLight 755	AF2290Z
FITC	AF2290F
HRP	AF2290H
Janelia Fluor 525	AF2290JF525
Janelia Fluor 549	AF2290I
Janelia Fluor 646	AF2290J
Janelia Fluor 669	AF2290JF669
PE	AF2290P
PerCP	AF2290C
mFluor Violet 450 SE	AF2290MFV450
mFluor Violet 500 SE	AF2290MFV500
mFluor Violet 610 SE	AF2290MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2290-SP

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NBP3-13705

ATM [p Ser1981] Antibody (HL1062) - Azide and BSA Free

100 ul

NBP3-13705

Immunohistochemistry-Paraffin: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (-) and treated (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-13655

ATM Antibody (2C1-RB) - Azide and BSA Free

100 ul

NBP3-13655

<b>Genetic Strategies Validation. </b>Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB22902

ATM [p Ser1981] Antibody (1163B) [Unconjugated]

100 μg

MAB22902

HAF008). A specific band was detected for phospho-ATM (S1981) at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF008). A specific band was detected for phospho-ATM (S1981) at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-ATM (S1981) Monoclonal Antibody (Catalog # MAB22902) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-ATM (S1981) at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB22902U-100UG
Alexa Fluor 405	FAB22902V-100UG
Alexa Fluor 488	FAB22902G-100UG
Alexa Fluor 532	FAB22902X-100UG
Alexa Fluor 594	FAB22902T-100UG
Alexa Fluor 647	FAB22902R-100UG
Alexa Fluor 700	FAB22902N-100UG
Alexa Fluor 750	FAB22902S-100UG
Alexa Fluor Plus 405	FAB22902AFP405-100UG
Alexa Fluor Plus 488	FAB22902AFP488-100UG
Alexa Fluor Plus 555	FAB22902AFP555-100UG
Alexa Fluor Plus 594	FAB22902AFP594-100UG
Alexa Fluor Plus 647	FAB22902AFP647-100UG
Alexa Fluor Plus 680	FAB22902AFP680-100UG
Allophycocyanin	FAB22902A
Biotin	FAB22902B
CoraFluor 1	FAB22902CL1
DyLight 350	FAB22902D
DyLight 405	FAB22902E
DyLight 488	FAB22902K
DyLight 550	FAB22902L
DyLight 594	FAB22902M
DyLight 650	FAB22902W
DyLight 680	FAB22902Y
DyLight 755	FAB22902Z
FITC	FAB22902F
HRP	FAB22902H
Janelia Fluor 525	FAB22902JF525
Janelia Fluor 549	FAB22902I
Janelia Fluor 646	FAB22902J
Janelia Fluor 669	FAB22902JF669
PE	FAB22902P
PerCP	FAB22902C
mFluor Violet 450 SE	FAB22902MFV450
mFluor Violet 500 SE	FAB22902MFV500
mFluor Violet 610 SE	FAB22902MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB22902-SP

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NBP3-26493

ATM Antibody (4E11) - BSA Free

100 ul

NBP3-26493

Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate.<br /> All lanes: ATM Antibody at 2.05ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 350 KDa<br /> Observed band size: 350 KDa<br />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-26493-50ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

ATM Antibodies

ATM Antibody (2C1) - Azide and BSA Free

ATM Antibody - BSA Free

ATM [p Ser1981] Antibody (10H11.E12) - BSA Free

ATM [p Ser1981] Antibody

ATM Antibody (5C2) - Azide and BSA Free

ATM Antibody - BSA Free

ATM Antibody - BSA Free

ATM Antibody [Unconjugated]

ATM [p Ser1981] Antibody (HL1062) - Azide and BSA Free

ATM Antibody (2C1-RB) - Azide and BSA Free

ATM [p Ser1981] Antibody (1163B) [Unconjugated]

ATM Antibody (4E11) - BSA Free