Raji Whole Cell Lysate

Images

 
Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear ...read more
SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

Raji Whole Cell Lysate Summary

Specificity
Total Protein - Human Tumor Cell Line: Raji

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Blood Lymph
Protein State
Native
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Raji Lysate (NB820-59466). (Showing 1 - 1 of 1 FAQ).

  1. Were the cells lysed immediately after collection, or was the cell pellet frozen and lysed at a later date?
    • The lysates are prepared from frozen cell pellets.

Additional Raji Products

Array NB820-59466

Blogs on Raji

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Contact Information

Product PDFs

Calculators

Concentration Calculator

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