CARD12 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CARD12 Antibody [NBP1-78979] - The CARD12 antibody (NBP1-78979) was tested in HepG2 cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunohistochemistry: CARD12 Antibody [NBP1-78979] - IHC analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.13 mg/ml

CARD12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CARD12 Antibody - BSA Free (NBP1-78979) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-CARD12 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of the human CARD12 protein (between residues 600-700) [UniProt Q9NPP4]
• Localization: Cytoplasm. Note: Cytoplasmic filaments.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 29104144)
  • Immunoblotting reported in scientific literature (PMID 29104144)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.13 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CARD12 Antibody - BSA Free

• CARD12leucine rich repeat and CARD domaincontaining 4
• caspase recruitment domain family, member 12
• Caspase recruitment domain-containing protein 12
• Clan protein
• CLAN1ipaf
• CLANCARD, LRR, and NACHT-containing protein
• CLR2.1
• Ice protease-activating factor
• Ipaf
• NLR family, CARD domain containing 4

Background

CARD12, also known as CLAN, IPAF and CLAN1 is a member of the CED4 family of proteins. The CED4/Apaf-1 proteins are critical regulators of apoptosis and NF-kappaB signaling pathways, and CARD12 is no exception. CARD12 has been implicated in coordination of downstream activation of both apoptotic and inflammatory signaling pathways (PMID: 11374873). Additionally, CARD12 has shown to coprecipitate with caspase 1, an enzyme that participates in both the signaling of apoptosis and cytokine processing, further demonstrating its integral role in programmed cell death (PMID: 11374873). As part of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins, CARD12 is known to be an important regulator of caspase-1 activation via an ATP/dATP nucleotide-binding pocket (PMID: 15882992). CARD12 has four known isoforms. Isoform 1 is highly expressed in lung, followed by leukocytes (especially monocytes), lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Although it is expressed ubiquitously, Isoform 2 is more prominent at the highest levels in lung and spleen. Isoform 4 can only been found in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CARD12 Antibody (NBP1-78979)(2)

Publications using NBP1-78979

• Wu, Q, Wang, B Et al. Bacterial Type I CRISPR-Cas systems influence inflammasome activation in mammalian host by promoting autophagy. Immunology 2019-11-01 [PMID: 31429483] (WB, ICC/IF, Human)
• Jabir MS, Sulaiman GM, Taqi ZJ, Li D. Iraqi propolis increases degradation of IL-1beta and NLRC4 by autophagy following Pseudomonas aeruginosa infection. Microbes Infect 2018-02-01 [PMID: 29104144]

Bioinformatics

• Gene Symbol: NLRC4
• Entrez:
  • 58484(Human)
• Uniprot:
  • Q9NPP4(Human)