DNMT3B Antibody

Images

 
Western Blot: DNMT3B Antibody [NB300-516] - Expression level of DNMT3B by RT-PCR and Western blot (W.B.) analysis in human oral keratinocyte (HOK) and OSCC cell lines. GAPDH and a-tubulin were used as internal control, ...read more
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary ...read more
Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.
Biological Strategies: Western Blot: DNMT3B Antibody [NB300-516] - Western blot analysis of DNMT3B level in wildtype DOK cells (Wt), Are-L and NNK-L DOK cells after treatment with 5-aza-dC (5uM) for 5 days. ...read more
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary ...read more
Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.
Genetic Strategies: Knockdown Validated: DNMT3B Antibody [NB300-516] - RT-PCR and Western blot analysis of DNMT3B in SCC-15 and OEC-M1 cells following DNMT3B knockdown (si-DNMT3B) or non-targeting siRNA control ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNMT3B Antibody Summary

Immunogen
Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b
Localization
Nuclear
Specificity
This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Knockdown Validated
  • Simple Western 20 ug/mL
  • Western Blot 2.0 ug/mL
Application Notes
May be Useful in WB. ChIP usage was reported in scientific literature. WB: Detects a 130 kDa band is seen in P19 nuclear extracts.
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB300-516 in the following applications:

Publications
Read Publications using
NB300-516 in the following applications:

  • 3 publications
  • WB
    6 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218) Human reactivity reported in scientific literature (PMID: 20847044). Mouse reactivity reported in scientific literature (PMID: 21149390)..

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNMT3B Antibody

  • DNA (cytosine-5-)-methyltransferase 3 beta
  • DNA (cytosine-5)-methyltransferase 3B
  • DNA methyltransferase HsaIIIB
  • DNA MTase HsaIIIB
  • DNMT3B
  • EC 2.1.1.37
  • ICF
  • ICF1
  • M.HsaIIIB

Background

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB

Publications for DNMT3B Antibody (NB300-516)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(3)
WB
(6)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB300-516 Applications Species
Liu X, Cui H The palliative effects of folic acid on retinal microvessels in diabetic retinopathy via regulating the metabolism of DNA methylation and hydroxymethylation Bioengineered Dec 1 2021 [PMID: 34874218] (ChIP, Rat) ChIP Rat
Huang Z, Yu J, Cui W, et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins Science Advances Jan 1 2021 [PMID: 33523915] (WB, Mouse) WB Mouse
Go S, Kurita H, Hatano M, et al. DNA methyltransferase- and histone deacetylase-mediated epigenetic alterations induced by low-level methylmercury exposure disrupt neuronal development Archives of toxicology Jan 16 2021 [PMID: 33454822]
Liu N, Zhang XL, Jiang SY et al. Neuroprotective mechanisms of DNA methyltransferase in a mouse hippocampal neuronal cell line after hypoxic preconditioning Neural Regen Res Dec 1 2020 [PMID: 32594061] (WB, Mouse) WB Mouse
Shiah SG, Hsiao JR, Chang HJ et al. MiR-30a and miR-379 modulate retinoic acid pathway by targeting DNA methyltransferase 3B in oral cancer J. Biomed. Sci. Apr 2 2020 [PMID: 32238162] (WB, Human) WB Human
Arab K, Karaulanov E, Musheev M et al. GADD45A binds R-loops and recruits TET1 to CpG island promoters. Nat. Genet. Feb 1 2019 [PMID: 30617255] (ChIP, Human) ChIP Human
Ponnaluri VKC, Esteve PO, Ruse CI, Pradhan S. S-adenosylhomocysteine Hydrolase Participates in DNA Methylation Inheritance J. Mol. Biol. Jul 6 2018 [PMID: 29758262] (WB) WB
Zhong T, Men Y, Lu L et al. Metformin alters DNA methylation genome-wide via the H19/SAHH axis. Oncogene Oct 24 2016 [PMID: 27775072] (Human) Human
Zhou J, Yang L, Zhong T et al. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. Nat Commun. 2015 Dec 21 [PMID: 26687445] (WB, Human) WB Human
Jin B, Ernst J et al. Linking DNA methyltransferases to epigenetic marks and nucleosome structure genome-wide in human tumor cells. Cell Rep Nov 29 2012 [PMID: 23177624] (WB, Human) WB Human
Show All 13 Publications.

Reviews for DNMT3B Antibody (NB300-516) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-516:
Filter by Applications
Simple Western
(1)
Chromatin Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western DNMT3B NB300-516
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1
reviewed by:
Verified Customer
Simple Western Human 02/03/2020
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Summary

ApplicationSimple Western
Sample TestedHepG2 CELLS
SpeciesHuman
LotTH266870

Comments

Commentsmateriel: 1x25 caps 12-230kDa
échantillon : 0.08 à 1µg/µL
anticorps : 1/250 à 1/10
Chromatin Immunoprecipitation DNMT3B NB300-516
Enlarge
5
reviewed by:
Verified Customer
Chromatin Immunoprecipitation Human 01/30/2019
View

Summary

ApplicationChromatin Immunoprecipitation
Sample Testedcell
SpeciesHuman
LotTE264897
Western Blot DNMT3B NB300-516
Enlarge
5
reviewed by:
Sara Smith
Western Blot Mouse 07/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedLysate from homogenized mouse brain cells
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for DNMT3B Antibody (NB300-516) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on DNMT3B.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

DNMT3B: Roles in Leukemia
DNA-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB, is a member of the class I-like SAM-binding methyltransferase superfamily and C5-methyltransferase family. DNMT3B plays an essential role in the establishment of DNA methy...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

3.7
5
2
4
0
3
0
2
0
1
1

Verified Customer
02/03/2020
Application: Simple Western
Species: Human

Verified Customer
01/30/2019
Application: Chromatin Immunoprecipitation
Species: Human

Sara Smith
07/17/2014
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol DNMT3B
Entrez
Uniprot