DNMT3B Antibody

Images

 
Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary ...read more
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary ...read more
Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNMT3B Antibody Summary

Immunogen
Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b
Localization
Nuclear
Specificity
This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNMT3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/mL
  • Simple Western 20 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
Application Notes
May be Useful in WB. ChIP usage was reported in scientific literature. WB: Detects a 130 kDa band is seen in P19 nuclear extracts.
Control
DNMT3B Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB300-516 in the following applications:

Publications
Read Publications using
NB300-516 in the following applications:

  • 2 publications
  • WB
    4 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20847044). Mouse reactivity reported in scientific literature (PMID: 21149390).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNMT3B Antibody

  • DNA (cytosine-5-)-methyltransferase 3 beta
  • DNA (cytosine-5)-methyltransferase 3B
  • DNA methyltransferase HsaIIIB
  • DNA MTase HsaIIIB
  • DNMT3B
  • EC 2.1.1.37
  • ICF
  • ICF1
  • M.HsaIIIB

Background

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has also been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3B Antibody (NB300-516)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


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(2)
WB
(4)
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(4)
Mouse
(2)
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Showing Publications 1 - 9 of 9.
Publications using NB300-516 Applications Species
Liu N, Zhang XL, Jiang SY et al. Neuroprotective mechanisms of DNA methyltransferase in a mouse hippocampal neuronal cell line after hypoxic preconditioning Neural Regen Res Dec 1 2020 [PMID: 32594061] (WB, Mouse) WB Mouse
Shiah SG, Hsiao JR, Chang HJ et al. MiR-30a and miR-379 modulate retinoic acid pathway by targeting DNA methyltransferase 3B in oral cancer J. Biomed. Sci. Apr 2 2020 [PMID: 32238162] (WB, Human) WB Human
Arab K, Karaulanov E, Musheev M et al. GADD45A binds R-loops and recruits TET1 to CpG island promoters. Nat. Genet. Feb 1 2019 [PMID: 30617255] (ChIP, Human) ChIP Human
Ponnaluri VKC, Esteve PO, Ruse CI, Pradhan S. S-adenosylhomocysteine Hydrolase Participates in DNA Methylation Inheritance J. Mol. Biol. Jul 6 2018 [PMID: 29758262] (WB) WB
Zhong T, Men Y, Lu L et al. Metformin alters DNA methylation genome-wide via the H19/SAHH axis. Oncogene Oct 24 2016 [PMID: 27775072] (Human) Human
Zhou J, Yang L, Zhong T et al. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. Nat Commun. 2015 Dec 21 [PMID: 26687445] (WB, Human) WB Human
Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Pierre Issa J, Orgebin-Crist MC, Wilkinson MF. DNA Demethylation-Dependent AR Recruitment and GATA Factors Drive Rhox5 Homeobox Gene Transcription in the Epididymis. Mol Endocrinol;26(4):538-49. 2012 Apr. [PMID: 22322598] (ChIP, Mouse) ChIP Mouse
Jin B, Tao Q, Peng J, Soo HM, Wu W, Ying J, Fields CR, Delmas AL, Liu X, Qiu J, Robertson KD. DNA methyltransferase 3B (DNMT3B) mutations in ICF syndrome lead to altered epigenetic modifications and aberrant expression of genes regulating development, neurogenesis and immune function. Hum Mol Genet;17(5):690-709. 2008 Mar 1. [PMID: 18029387]
Myant K, Stancheva I. LSH cooperates with DNA methyltransferases to repress transcription. Mol Cell Biol. 2008 Jan. [PMID: 17967891]

Reviews for DNMT3B Antibody (NB300-516) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-516:
Filter by Applications
Simple Western
(1)
ChIP
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western DNMT3B NB300-516
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1
reviewed by:
Jeremie RASO
Simple Western Human 02/03/2020
View

Summary

ApplicationSimple Western
Sample TestedHepG2 CELLS
SpeciesHuman
LotTH266870

Comments

Commentsmateriel: 1x25 caps 12-230kDa
échantillon : 0.08 à 1µg/µL
anticorps : 1/250 à 1/10
Chromatin Immunoprecipitation DNMT3B NB300-516
Enlarge
5
reviewed by:
Yichi Xu
ChIP Human 01/30/2019
View

Summary

ApplicationChromatin Immunoprecipitation
Sample Testedcell
SpeciesHuman
LotTE264897
Western Blot DNMT3B NB300-516
Enlarge
5
reviewed by:
Anonymous
WB Mouse 07/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedLysate from homogenized mouse brain cells
SpeciesMouse
CommentsDnmt3b is a 97 KDa protein but this antibody (as all other Dnmt3b antibodies) detects a band of approx 130KDa in mouse brain cell lysate

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio1000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7074
Secondary Concentration1:20K

Details

Detection NotesCaptured using X-rays (20 sec exposure) with Phototope-HRP Western Blot Detection System

Comments

CommentsDnmt3b is a 97 KDa protein but this antibody (as all other Dnmt3b antibodies) detects a band of approx 130KDa in mouse brain cell lysate

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for DNMT3B Antibody (NB300-516) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DNMT3B Antibody (NB300-516)

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Research Areas for DNMT3B Antibody (NB300-516)

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Blogs on DNMT3B.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

DNMT3B: Roles in Leukemia
DNA-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB, is a member of the class I-like SAM-binding methyltransferase superfamily and C5-methyltransferase family. DNMT3B plays an essential role in the establishment of DNA methy...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

3.7
5
2
4
0
3
0
2
0
1
1

Jeremie RASO
02/03/2020
Application: Simple Western
Species: Human

Yichi Xu
01/30/2019
Application: ChIP
Species: Human

Anonymous
07/17/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol DNMT3B
Entrez
Uniprot