Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of dissociated rat hippocampal culture. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human pancreas shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review.
  • Immunohistochemistry Free-Floating Reported in scientific literature (PMID: 31785263).
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 24658304).
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot Reported in scientific literature (PMID: 24658304).
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Muscarinic Acetylcholine Receptor M1/CHRM1 Protein (NBP1-87466PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31785263). Use in Rat reported in scientific literature (PMID:31785263).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - BSA Free

• cholinergic receptor, muscarinic 1
• CHRM1
• HM1
• M1
• M1R
• mAChR M1
• MGC30125
• muscarinic acetylcholine receptor M1

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody (NBP1-87466)(5)

Publications using NBP1-87466

• Sarah H Targeting the M1 muscarinic acetylcholine receptor in neurodegeneration Thesis 2023-01-01 (Western Blot)
• Roberto Cadeddu, Giulia Braccagni, Caterina Branca, Easton R van Luik, Christopher Pittenger, Morten Skøtt Thomsen, Marco Bortolato Activation of M 4 muscarinic receptors in the striatum reduces tic-like behaviours in two distinct murine models of Tourette syndrome. British journal of pharmacology 2024-04-30 [PMID: 38689378]
• Goto Y, Ando T, Izumi H et al. Muscarinic receptors promote castration-resistant growth of prostate cancer through a FAK-YAP signaling axis Oncogene 2020-03-23 [PMID: 32205868] (ICC/IF, Human)
• Cieslik P, Domin H, Chocyk A et al. Simultaneous activation of mGlu2 and muscarinic receptors reverses MK-801-induced cognitive decline in rodents Neuropharmacology 2019-11-27 [PMID: 31785263] (IHC-FrFl, Mouse, Rat)
• Molina J, Rodriguez-Diaz R, Fachado A et al. Control of Insulin Secretion by Cholinergic Signaling in the Human Pancreatic Islet. Diabetes 2014-08-01 [PMID: 24658304] (IHC-Fr, Human)

Review for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody (NBP1-87466) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Bryan Tinsley ICC Rat 08/27/2014

Summary

• Application: Immunocytochemistry
• Species: Rat

Bioinformatics

• Gene Symbol: CHRM1