Cathepsin L Antibody [Unconjugated]

Images

 
Western blot shows 10 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, ...read more
Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Cathepsin L Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
Glu113-Val333
Accession # P07711
Specificity
Detects human Cathepsin L in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant mouse Cathepsin L, recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin E, rhCathepsin S, and rhCathepsin V is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSL1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Cathepsin L Antibody (Catalog # AF952)
ELISA Detection: Human Cathepsin L Biotinylated Antibody (Catalog # BAF952)
Standard: Recombinant Human Cathepsin L (Catalog # 952-CY)
Control
Cathepsin L Knockout 293T Cell Lysate
Publications
Read Publications using
AF952 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody [Unconjugated]

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5‑6.5. The stability of the enzyme decreases at higher pH values.

  1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
  2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF953
Species: Hu
Applications: WB, Simple Western, IHC, IP, KO
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-78977
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IM
AF1013
Species: Mu
Applications: WB, IHC, IP
H00001513-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF1029
Species: Mu
Applications: WB, IHC, IP, ICC
AF1183
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-45861
Species: Hu
Applications: WB, Flow, IHC, IF
NB600-1397
Species: Hu, Mu, Bv, Ca, Mk, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, MiAr, CyTOF-ready, Flow-IC
NBP2-58661
Species: Hu
Applications: IHC, IHC-P
MAB1080
Species: Hu
Applications: WB, IHC, IP
NBP1-87466
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2340
Species: Hu
Applications: WB, Flow, IP, CyTOF-ready
AF3749
Species: Hu
Applications: WB, IHC
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
AF1286
Species: Hu
Applications: WB, Simple Western, IHC, IP

Publications for Cathepsin L Antibody (AF952)(7)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Chicken
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF952 Applications Species
M Flint, P Chatterjee, DL Lin, LK McMullan, P Shrivastav, É Bergeron, MK Lo, SR Welch, ST Nichol, AW Tai, CF Spiropoulo A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Nat Commun, 2019;10(1):285. 2019 [PMID: 30655525] (Western Blot, Human) Western Blot Human
CW Lee, JN Stankowski, J Chew, CN Cook, YW Lam, S Almeida, Y Carlomagno, KF Lau, M Prudencio, FB Gao, M Bogyo, DW Dickson, L Petrucelli The lysosomal protein cathepsin L is a progranulin protease Mol Neurodegener, 2017;12(1):55. 2017 [PMID: 28743268] (Western Blot, Human) Western Blot Human
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (Western Blot, Chicken) Western Blot Chicken
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (Western Blot, Human) Western Blot Human
Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014;9(1):e85721. 2014 [PMID: 24416446] (Western Blot, Human) Western Blot Human
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer, 2010;10(1):17. 2010 [PMID: 20074384] (Western Blot, Human) Western Blot Human
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev., 2006;20(5):543-56. 2006 [PMID: 16481467] (IHC-P, Human) IHC-P Human

Reviews for Cathepsin L Antibody (AF952) (0)

There are no reviews for Cathepsin L Antibody (AF952). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cathepsin L Antibody (AF952) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF952

Additional Cathepsin L Products

Bioinformatics Tool for Cathepsin L Antibody (AF952)

Discover related pathways, diseases and genes to Cathepsin L Antibody (AF952). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cathepsin L Antibody (AF952)

Discover more about diseases related to Cathepsin L Antibody (AF952).
 

Pathways for Cathepsin L Antibody (AF952)

View related products by pathway.

PTMs for Cathepsin L Antibody (AF952)

Learn more about PTMs related to Cathepsin L Antibody (AF952).

Blogs on Cathepsin L

There are no specific blogs for Cathepsin L, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cathepsin L Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol CTSL1
Uniprot