Cathepsin L Antibody [Unconjugated]

Western blot shows 10 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin L Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
  Glu113-Val333
  Accession # P07711
• Specificity: Detects human Cathepsin L in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTSL1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Cathepsin L Antibody (Catalog # AF952)
  ELISA Detection: Human Cathepsin L Biotinylated Antibody (Catalog # BAF952)
  Standard: Recombinant Human Cathepsin L (Catalog # 952-CY)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody [Unconjugated]

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha ₁-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5‑6.5. The stability of the enzyme decreases at higher pH values.

1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (AF952)(15)

Publications using AF952

• K Chan, AG Farias, H Lee, F Guvenc, P Mero, KR Brown, H Ward, M Billmann, K Aulakh, A Astori, S Haider, E Marcon, U Braunschwe, S Pu, A Habsid, AH Yan Tong, N Christie-H, P Budylowski, A Ghalami, S Mubareka, F Maguire, A Banerjee, KL Mossman, J Greenblatt, SD Gray-Owen, B Raught, BJ Blencowe, M Taipale, C Myers, J Moffat Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors Heliyon, 2022;0(0):e12744. 2022 [PMID: 36597481] (Western Blot, Human)
• BJ Willett, J Grove, OA MacLean, C Wilkie, G De Lorenzo, W Furnon, D Cantoni, S Scott, N Logan, S Ashraf, M Manali, A Szemiel, V Cowton, E Vink, WT Harvey, C Davis, P Asamaphan, K Smollett, L Tong, R Orton, J Hughes, P Holland, V Silva, DJ Pascall, K Puxty, A da Silva F, G Yebra, S Shaaban, MTG Holden, RM Pinto, R Gunson, K Templeton, PR Murcia, AH Patel, P Klenerman, S Dunachie, PITCH Cons, COVID-19 G, J Haughney, DL Robertson, M Palmarini, S Ray, EC Thomson SARS-CoV-2 Omicron is an immune escape variant with an altered cell entry pathway Nature Microbiology, 2022;0(0):. 2022 [PMID: 35798890] (Western Blot, Human)
• A Saidi, P Gaboriaud, AC Lalmanach, L Vanderlynd, A Fessard, P Vettori, G Fort, R Guabiraba, C Schouler, F Laurent, E Guitton, F Lecaille, FI Bussière, G Lalmanach Upregulation of gut cathepsin L during Eimeria tenella infection Research in Veterinary Science, 2021;140(0):109-116. 2021 [PMID: 34419895] (Western Blot, Chicken)
• L Liu, B Doray Elevated mRNA expression and defective processing of cathepsin D in HeLa cells lacking the mannose 6-phosphate pathway FEBS Open Bio, 2021;0(0):. 2021 [PMID: 33932147] (Western Blot, Human)
• D Oelschlaeg, T Weiss Sada, S Salpeter, S Krug, G Blum, W Schmitz, A Schulze, P Michl Cathepsin Inhibition Modulates Metabolism and Polarization of Tumor-Associated Macrophages Cancers (Basel), 2020;12(9):. 2020 [PMID: 32927704] (IHC, Human)
• K Wang, Y Zhan, B Chen, Y Lu, T Yin, S Zhou, W Zhang, X Liu, B Du, X Wei, J Xiao Tubeimoside I-induced lung cancer cell death and the underlying crosstalk between lysosomes and mitochondria Cell Death Dis, 2020;11(8):708. 2020 [PMID: 32848130] (Western Blot, Human)
• K Wang, Y Zhan, B Chen, Y Lu, T Yin, S Zhou, W Zhang, X Liu, B Du, X Wei, J Xiao Tubeimoside I-induced lung cancer cell death and the underlying crosstalk between lysosomes and mitochondria Cell Death Dis, 2020;11(8):708. 2020 [PMID: 32848130] (ICC, Human)
• MJ Kobylarz, JM Goodwin, ZB Kang, JW Annand, S Hevi, E O'Mahony, G McAllister, J Reece-Hoye, Q Wang, J Alford, C Russ, A Lindeman, M Beibel, G Roma, W Carbone, J Knehr, J Loureiro, C Antczak, D Wiederscha, LO Murphy, S Menon, B Nyfeler An iron-dependent metabolic vulnerability underlies VPS34-dependence in RKO cancer cells PLoS ONE, 2020;15(8):e0235551. 2020 [PMID: 32833964] (Western Blot, Human)
• M Flint, P Chatterjee, DL Lin, LK McMullan, P Shrivastav, É Bergeron, MK Lo, SR Welch, ST Nichol, AW Tai, CF Spiropoulo A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Nat Commun, 2019;10(1):285. 2019 [PMID: 30655525] (Western Blot, Human)
• CW Lee, JN Stankowski, J Chew, CN Cook, YW Lam, S Almeida, Y Carlomagno, KF Lau, M Prudencio, FB Gao, M Bogyo, DW Dickson, L Petrucelli The lysosomal protein cathepsin L is a progranulin protease Mol Neurodegener, 2017;12(1):55. 2017 [PMID: 28743268] (Western Blot, Human)
• The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (Western Blot, Chicken)
• Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (Western Blot, Human)
• Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014;9(1):e85721. 2014 [PMID: 24416446] (Western Blot, Human)
• Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer, 2010;10(1):17. 2010 [PMID: 20074384] (Western Blot, Human)
• Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev., 2006;20(5):543-56. 2006 [PMID: 16481467] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CTSL1
• Uniprot:
  • P07711()