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Cathepsin L Antibody [Unconjugated]

Images

 
Western blot shows 10 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, ...read more
Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Cathepsin L Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
Glu113-Val333
Accession # P07711
Specificity
Detects human Cathepsin L in ELISAs and Western blots. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSL1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Cathepsin L Antibody (Catalog # AF952)
ELISA Detection: Human Cathepsin L Biotinylated Antibody (Catalog # BAF952)
Standard: Recombinant Human Cathepsin L (Catalog # 952-CY)
Publications
Read Publications using
AF952 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody [Unconjugated]

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5‑6.5. The stability of the enzyme decreases at higher pH values.

  1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
  2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin L Antibody (AF952)(11)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(9)
Chicken
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF952 Applications Species
A Saidi, P Gaboriaud, AC Lalmanach, L Vanderlynd, A Fessard, P Vettori, G Fort, R Guabiraba, C Schouler, F Laurent, E Guitton, F Lecaille, FI Bussière, G Lalmanach Upregulation of gut cathepsin L during Eimeria tenella infection Research in Veterinary Science, 2021;140(0):109-116. 2021 [PMID: 34419895] (Western Blot, Chicken) Western Blot Chicken
L Liu, B Doray Elevated mRNA expression and defective processing of cathepsin D in HeLa cells lacking the mannose 6-phosphate pathway FEBS Open Bio, 2021;0(0):. 2021 [PMID: 33932147] (Western Blot, Human) Western Blot Human
D Oelschlaeg, T Weiss Sada, S Salpeter, S Krug, G Blum, W Schmitz, A Schulze, P Michl Cathepsin Inhibition Modulates Metabolism and Polarization of Tumor-Associated Macrophages Cancers (Basel), 2020;12(9):. 2020 [PMID: 32927704] (IHC, Human) IHC Human
K Wang, Y Zhan, B Chen, Y Lu, T Yin, S Zhou, W Zhang, X Liu, B Du, X Wei, J Xiao Tubeimoside I-induced lung cancer cell death and the underlying crosstalk between lysosomes and mitochondria Cell Death Dis, 2020;11(8):708. 2020 [PMID: 32848130] (Western Blot, Human) Western Blot Human
M Flint, P Chatterjee, DL Lin, LK McMullan, P Shrivastav, É Bergeron, MK Lo, SR Welch, ST Nichol, AW Tai, CF Spiropoulo A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Nat Commun, 2019;10(1):285. 2019 [PMID: 30655525] (Western Blot, Human) Western Blot Human
CW Lee, JN Stankowski, J Chew, CN Cook, YW Lam, S Almeida, Y Carlomagno, KF Lau, M Prudencio, FB Gao, M Bogyo, DW Dickson, L Petrucelli The lysosomal protein cathepsin L is a progranulin protease Mol Neurodegener, 2017;12(1):55. 2017 [PMID: 28743268] (Western Blot, Human) Western Blot Human
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (Western Blot, Chicken) Western Blot Chicken
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (Western Blot, Human) Western Blot Human
Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014;9(1):e85721. 2014 [PMID: 24416446] (Western Blot, Human) Western Blot Human
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer, 2010;10(1):17. 2010 [PMID: 20074384] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol CTSL1
Uniprot