ASC/TMS1: Novus Biologicals

NBP1-78977

ASC/TMS1 Antibody - BSA Free

(2 Reviews)

(53 Publications)

0.1 ml

NBP1-78977

<b>Biological Strategies Validation. </b>Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.

Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-ACS/TMS1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Flow Cytometry: ASC/TMS1 Antibody - BSA Free [NBP1-78977] - An intracellular stain was performed on MCF7 cells with ASC/TMS1 NBP1-78977 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.

Nicorandil alleviates cardiac apoptosis in type 2 diabetic rat. A: TUNEL staining and TUNEL‐positive cells rate. B: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3. C: Level of nitric oxide and ADMA in serum. D: Western blot analysis of p‐eNOS. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. *P < 0.05 compared with control; #P < 0.05 compared with DM; #P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PI3K/AKT pathway inhibition blocked the protection of nicorandil on H9c2 cardiomyocyte treated with high glucose. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors (scale bar: 20 um). C: Western blot analysis of p‐eNOS in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. N: Nicorandil (100 umol); MTF: miltefosine (100 umol); Rapa: rapamycin (100 umol) NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). *P < 0.05 compared with NG; #P < 0.05 compared with HG; &P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Nicorandil protects H9C2 cells from apoptosis through PI3K/AKT pathway. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and 5‐HD which is a inhibitor of nicorandil. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor（5‐HD, 500 umol) (scale bar: 20 um). I:NG, II:HG, III:HG + N, IV:HG + N+5‐HD; C: Western blot analysis of phosphorylation level of PI3K, AKT, eNOS and mTOR in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor（5‐HD). N: Nicorandil (100 umol); NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). *P < 0.05 compared with NG; #P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of abrocitinib on NF kappa B-related inflammation and pyroptosis pathways. On the 1st day after TBI, NF kappa B-related inflammation and the activation of pyroptosis pathways were dramatically increased. After abrocitinib treatment, the indicators of NF kappa B-related inflammation and pyroptosis pathways were significantly decreased, as can be seen from the WB (n = 5–9 one-way ANOVA with Tukey’s post-hoc test) and GSDMD IHC staining (A–C) (n = 6 Kruskal-Wallis test). From the ELISA results, the changes in inflammatory cytokines (IL-1 beta and IL-18) after brain injury and the effects of abrocitinib were precisely revealed (D,E) (n = 5–6 one-way ANOVA with Tukey’s post-hoc test). All data are shown as mean +/- SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Apoptosis level reduced after nicorandil treatment in high glucose‐induced H9c2 cardiomyocyte. A: Western blot analysis of bax and bcl‐2 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment with different concentrations for 24 h. B: Western bolt analysis of cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment. HG (33.3 mmol/L), NG (5.5 mmol/L), n1: nicorandil (10 umol); n2: nicorandil (50 umol); n3: nicorandil (100 umol). #P < 0.05 compared with NG; *P < 0.05 compared with HG, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of Mito-Vit-E and TLR9 inhibitor OND-I in LPS-challenged cardiomyocytes.Cultured neonatal cardiomyocytes from rats were treated with +/-LPS (100 ng/ml), +/-Mito-Vit-E (MVE) (1μM), or +/-ODN-I (0.5 μM) 4 hours prior to harvesting. A. Mitochondrial superoxide was labeled with MitoSox Red and quantified by flow cytometry. B. Mitochondrial biogenesis was quantified in live cells using MitoBiogenesis In-Cell ELISA assay. C. Levels of mtDNA in cell medium and in cytoplasm were measured by real-time PCR. D. Cells apoptosis was evaluated by TUNEL assay (green). Cell nucleuses were identified by DAPI staining (blue). E. Expression of MyD88, RAGE, ASC and activated form of caspase 1 were determined in cell lysates by western blot using GAPDH as a loading control, and results were quantified by densitometry. F. Cellular production of IL–1 beta was measured by ELISA. All the measurements were normalized by cell numbers and obtained in triplicate. All values are means +/-SE. Significant differences are shown as * between control and LPS and delta between vehicle and drug-treated groups (p<0.02 for A-C and p<0.01 for E-F, n = 4). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26448624), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, beta -actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH, AIM2, ASC, Caspase-4 and beta -actinin UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NLRP3 (B), NLRC4 (C), NAIP (D), AIM2 (E), ASC (F), Caspase-4 (G), and Caspase-1 (H). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ∗∗∗p = 0.0001 and n.s, non-significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

QWZK inhibited NLRP3 inflammasome activation in ALI rats induced by LPS. (A) Western blot assay of NLRP3, pro-caspase-1, cleaved caspase-1, and ASC in different groups. (B–D) The protein expression was analyzed by gray scale. Data were presented as the mean +/- SEM, n = 8. #p < 0.05 vs. control group, ##p < 0.01 vs. control group, ###p < 0.001 vs. control group. *p < 0.05 vs. LPS group, **p < 0.01 vs. LPS group, ***p < 0.001 vs. LPS group. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fphar.2021.790072/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Inhibition of FOXO1 or autophagy compromised the antioxidative and anti‐inflammatory actions of AVE in mice. (a, b) The selective FOXO1 inhibitor, AS, and the autophagy inhibitor, CQ, abrogated the immune‐regulatory effect of AVE on microglial polarization. (c) AS and CQ both inhibited AVE‐induced alleviation of microglial activation (IBA‐1 staining) and ROS generation (DHE staining) following LPS exposure. (d, e) Representative western blots (d) and statistical graphs (e) of the major components of NLRP3 inflammasomes. Scale bar = 50 μm. Data are means +/- SD (n = 6–7). *p < 0.05, **p < 0.01 compared to control group. +p < 0.05, ++p < 0.01 compared to LPS group. #p < 0.05, ##p < 0.01 compared to LPS + AVE group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34529881), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The measurement of pyroptosis-related markers after intervention in the mice. (A) IF staining (×200) (left) and the IOD of NLRP3 in GAS of db/db mice (n = 6). The protein expression levels of (B) NLRP3; (C) ASC; (D) Caspase-1 and (E) GSDMD were detected by western blot in the GAS of db/db mice (n = 6). Serum inflammatory markers (F) IL-1 beta and (G) IL-18 by ELISA (n = 8). (#) Significant difference compared with D group; (&) significant difference compared with DI group; ($) significant difference compared with DE group (p < 0.05). Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1420-3049/29/3/712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78977AF350
Alexa Fluor 405	NBP1-78977AF405
Alexa Fluor 488	NBP1-78977AF488
Alexa Fluor 532	NBP1-78977AF532
Alexa Fluor 594	NBP1-78977AF594
Alexa Fluor 647	NBP1-78977AF647
Alexa Fluor 700	NBP1-78977AF700
Alexa Fluor 750	NBP1-78977AF750
Alexa Fluor Plus 405	NBP1-78977AFP405
Alexa Fluor Plus 488	NBP1-78977AFP488
Alexa Fluor Plus 555	NBP1-78977AFP555
Alexa Fluor Plus 594	NBP1-78977AFP594
Alexa Fluor Plus 647	NBP1-78977AFP647
Alexa Fluor Plus 680	NBP1-78977AFP680
Allophycocyanin	NBP1-78977APC
Biotin	NBP1-78977B
CoraFluor 1	NBP1-78977CL1
DyLight 350	NBP1-78977UV
DyLight 405	NBP1-78977V
DyLight 488	NBP1-78977G
DyLight 550	NBP1-78977R
DyLight 594	NBP1-78977DL594
DyLight 650	NBP1-78977C
DyLight 680	NBP1-78977FR
DyLight 755	NBP1-78977IR
FITC	NBP1-78977F
HRP	NBP1-78977H
Janelia Fluor 525	NBP1-78977JF525
Janelia Fluor 549	NBP1-78977JF549
Janelia Fluor 646	NBP1-78977JF646
Janelia Fluor 669	NBP1-78977JF669
PE	NBP1-78977PE
PerCP	NBP1-78977PCP
mFluor Violet 450 SE	NBP1-78977MFV450
mFluor Violet 500 SE	NBP1-78977MFV500
mFluor Violet 610 SE	NBP1-78977MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78977SS

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NBP1-78978

ASC/TMS1 Antibody - BSA Free

(1 Review)

(8 Publications)

0.1 ml

NBP1-78978

NLRP3 inflammasome and GSDMD are strongly expressed and activated in CNS microglia during EAE.A Immunoblot analysis of NLRP3, ASC, full-length (FL) and cleaved caspase-1, IL-1 beta , and GSDMD in the lumbosacral spinal cords of EAE-induced WT mice at peak disease or CFA-treated mice on day 18 after treatment (n = 10 per group). B Immunohistochemistry showing infiltration of NLRP3, ASC, caspase-1, IL-1 beta , and GSDMD-positive immune cells in the spinal cords of EAE-induced WT mice at peak disease or CFA-treated WT mice on day 18 after treatment. Scale bar: 50 um. n = 10 per group. C Immunofluorescent labeling of Iba-1 (green), NLRP3, ASC, caspase-1, or GSDMD (red), and DAPI (blue) demonstrates the expression and process of NLRP3 inflammasome and GSDMD-mediated pyroptosis in the microglia of WT EAE mice at peak disease or CFA-treated WT mice on day 18 after treatment (n = 10 per group). Scale bar: 20 um. Data are from three representative independent experiments and were analyzed by an unpaired t-test or the Mann–Whitney U test. Error bars show the mean +/- SEM. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36765034), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78978AF350
Alexa Fluor 405	NBP1-78978AF405
Alexa Fluor 488	NBP1-78978AF488
Alexa Fluor 532	NBP1-78978AF532
Alexa Fluor 594	NBP1-78978AF594
Alexa Fluor 647	NBP1-78978AF647
Alexa Fluor 700	NBP1-78978AF700
Alexa Fluor 750	NBP1-78978AF750
Alexa Fluor Plus 405	NBP1-78978AFP405
Alexa Fluor Plus 488	NBP1-78978AFP488
Alexa Fluor Plus 555	NBP1-78978AFP555
Alexa Fluor Plus 594	NBP1-78978AFP594
Alexa Fluor Plus 647	NBP1-78978AFP647
Alexa Fluor Plus 680	NBP1-78978AFP680
Allophycocyanin	NBP1-78978APC
Biotin	NBP1-78978B
CoraFluor 1	NBP1-78978CL1
DyLight 350	NBP1-78978UV
DyLight 405	NBP1-78978V
DyLight 488	NBP1-78978G
DyLight 550	NBP1-78978R
DyLight 594	NBP1-78978DL594
DyLight 650	NBP1-78978C
DyLight 680	NBP1-78978FR
DyLight 755	NBP1-78978IR
FITC	NBP1-78978F
HRP	NBP1-78978H
Janelia Fluor 525	NBP1-78978JF525
Janelia Fluor 549	NBP1-78978JF549
Janelia Fluor 646	NBP1-78978JF646
Janelia Fluor 669	NBP1-78978JF669
PE	NBP1-78978PE
PerCP	NBP1-78978PCP
mFluor Violet 450 SE	NBP1-78978MFV450
mFluor Violet 500 SE	NBP1-78978MFV500
mFluor Violet 610 SE	NBP1-78978MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78978SS

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AF3805

ASC/TMS1 Antibody [Unconjugated]

(6 Publications)

100 μg

AF3805

HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ASC at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for ASC at approximately 23 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of MCF-7 human breast cancer parental cell line and ASC knockout MCF-7 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for ASC at approximately 23 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1 beta and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

MiR-223-3p was capable of regulating NLRP3-mediated pyroptotic cell death in EPCs. (a) The eight miRNAs in BMSCs-exo were screened by using Real-Time qPCR, and (b) the process that BMSCs-exo delivered miR-223-3p into EPCs was examined. (c) The targeting sites in miR-223-3p and 3ʹ UTR of NLRP3 mRNA were predicted, and (d) those sites were validated by performing dual-luciferase reporter gene system assay. (e) miR-223-3p was overexpressed and silenced, and (f, g) the following experiments validated that miR-223-3p negatively regulated NLRP3 in EPCs cells. Upregulation of miR-223-3p suppressed LPS-induced (h) pyroptotic cell death, (i) cytokines generation and (j, k) secretion in EPCs cells. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

(A) NLRP3 inflammasome protein levels are displayed by Western blot in three different fibroblast cell populations: healthy fibroblasts (Healthy), psoriatic fibroblasts (PSO), and psoriatic fibroblasts infected with a pRRL-TTP-overexpressing vector (PSO TTP). Tubulin was used as loading control. (B) TTP overexpression is shown in psoriatic fibroblast sample. Actin was used as loading control. (C) Inflammasome components variation pattern already seen in primary fibroblasts is maintained in the HaCaT keratinocyte cell line. Actin was used as loading control. (D) pRRL-empty vector was used to obtain the overexpression vector pRRL-TTP, by substitution of delta NGFR sequence with TTP cDNA. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33585499), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3805U-100UG
Alexa Fluor 405	AF3805V-100UG
Alexa Fluor 488	AF3805G-100UG
Alexa Fluor 532	AF3805X-100UG
Alexa Fluor 594	AF3805T-100UG
Alexa Fluor 647	AF3805R-100UG
Alexa Fluor 700	AF3805N-100UG
Alexa Fluor 750	AF3805S-100UG
Alexa Fluor Plus 405	AF3805AFP405-100UG
Alexa Fluor Plus 488	AF3805AFP488-100UG
Alexa Fluor Plus 555	AF3805AFP555-100UG
Alexa Fluor Plus 594	AF3805AFP594-100UG
Alexa Fluor Plus 647	AF3805AFP647-100UG
Alexa Fluor Plus 680	AF3805AFP680-100UG
Allophycocyanin	AF3805A
Biotin	AF3805B
CoraFluor 1	AF3805CL1
DyLight 350	AF3805D
DyLight 405	AF3805E
DyLight 488	AF3805K
DyLight 550	AF3805L
DyLight 594	AF3805M
DyLight 650	AF3805W
DyLight 680	AF3805Y
DyLight 755	AF3805Z
FITC	AF3805F
HRP	AF3805H
Janelia Fluor 525	AF3805JF525
Janelia Fluor 549	AF3805I
Janelia Fluor 646	AF3805J
Janelia Fluor 669	AF3805JF669
PE	AF3805P
PerCP	AF3805C
mFluor Violet 450 SE	AF3805MFV450
mFluor Violet 500 SE	AF3805MFV500
mFluor Violet 610 SE	AF3805MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3805-SP

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NBP2-61682

ASC/TMS1 Antibody (1C3D7) - BSA Free

0.1 ml

NBP2-61682

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of stomach cancer tissues using PYCARD mouse mAb with DAB staining.

Flow Cytometry: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).

Western Blot: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of ovarian cancer tissues using PYCARD mouse mAb with DAB staining.

ELISA: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-61682-0.025ml

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NBP2-03818

ASC/TMS1 Antibody (OTI3E9)

(1 Publication)

0.1 ml

NBP2-03818

Flow Cytometry: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASC/TMS1 antibody, and then analyzed by flow cytometry.

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASC/TMS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASC/TMS1.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Human colon tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71853AF350
Alexa Fluor 405	NBP2-71853AF405
Alexa Fluor 488	NBP2-71853AF488
Alexa Fluor 532	NBP2-71853AF532
Alexa Fluor 594	NBP2-71853AF594
Alexa Fluor 647	NBP2-71853AF647
Alexa Fluor 700	NBP2-71853AF700
Alexa Fluor 750	NBP2-71853AF750
Alexa Fluor Plus 405	NBP2-71853AFP405
Alexa Fluor Plus 488	NBP2-71853AFP488
Alexa Fluor Plus 555	NBP2-71853AFP555
Alexa Fluor Plus 594	NBP2-71853AFP594
Alexa Fluor Plus 647	NBP2-71853AFP647
Alexa Fluor Plus 680	NBP2-71853AFP680
Allophycocyanin	NBP2-71853APC
Biotin	NBP2-71853B
CoraFluor 1	NBP2-71853CL1
DyLight 350	NBP2-71853UV
DyLight 405	NBP2-71853V
DyLight 488	NBP2-71853G
DyLight 550	NBP2-71853R
DyLight 594	NBP2-71853DL594
DyLight 650	NBP2-71853C
DyLight 680	NBP2-71853FR
DyLight 755	NBP2-71853IR
FITC	NBP2-71853F
HRP	NBP2-71853H
Janelia Fluor 525	NBP2-71853JF525
Janelia Fluor 549	NBP2-71853JF549
Janelia Fluor 646	NBP2-71853JF646
Janelia Fluor 669	NBP2-71853JF669
PE	NBP2-71853PE
PerCP	NBP2-71853PCP
mFluor Violet 450 SE	NBP2-71853MFV450
mFluor Violet 500 SE	NBP2-71853MFV500
mFluor Violet 610 SE	NBP2-71853MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71853

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NBP2-61676

ASC/TMS1 Antibody (1C3D3) - BSA Free

0.1 ml

NBP2-61676

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of stomach cancer tissues using PYCARD mouse mAb with DAB staining.

Flow Cytometry: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).

Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

ELISA: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-61676-0.025ml

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H00029108-P01

Recombinant Human ASC/TMS1 GST (N-Term) Protein

(4 Publications)

10 μg

H00029108-P01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

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NBP2-48925

ASC/TMS1 Antibody - BSA Free

0.1 ml

NBP2-48925

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP2-48925] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & cytosol.

Western Blot: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen tissue.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-77297

ASC/TMS1 Antibody - BSA Free

0.1 mg

NBP1-77297

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.

Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.

Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC/TMS1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC/TMS1 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5416

Alt Catalog# IMG-5416

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77297AF350
Alexa Fluor 405	NBP1-77297AF405
Alexa Fluor 488	NBP1-77297AF488
Alexa Fluor 532	NBP1-77297AF532
Alexa Fluor 594	NBP1-77297AF594
Alexa Fluor 647	NBP1-77297AF647
Alexa Fluor 700	NBP1-77297AF700
Alexa Fluor 750	NBP1-77297AF750
Alexa Fluor Plus 405	NBP1-77297AFP405
Alexa Fluor Plus 488	NBP1-77297AFP488
Alexa Fluor Plus 555	NBP1-77297AFP555
Alexa Fluor Plus 594	NBP1-77297AFP594
Alexa Fluor Plus 647	NBP1-77297AFP647
Alexa Fluor Plus 680	NBP1-77297AFP680
Allophycocyanin	NBP1-77297APC
Biotin	NBP1-77297B
CoraFluor 1	NBP1-77297CL1
DyLight 350	NBP1-77297UV
DyLight 405	NBP1-77297V
DyLight 488	NBP1-77297G
DyLight 550	NBP1-77297R
DyLight 594	NBP1-77297DL594
DyLight 650	NBP1-77297C
DyLight 680	NBP1-77297FR
DyLight 755	NBP1-77297IR
FITC	NBP1-77297F
HRP	NBP1-77297H
Janelia Fluor 525	NBP1-77297JF525
Janelia Fluor 549	NBP1-77297JF549
Janelia Fluor 646	NBP1-77297JF646
Janelia Fluor 669	NBP1-77297JF669
PE	NBP1-77297PE
PerCP	NBP1-77297PCP
mFluor Violet 450 SE	NBP1-77297MFV450
mFluor Violet 500 SE	NBP1-77297MFV500
mFluor Violet 610 SE	NBP1-77297MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77297-0.025mg

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NBP2-49133

ASC/TMS1 Antibody - BSA Free

0.1 ml

NBP2-49133

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human skeletal muscle shows low positivity in myocytes as expected.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-49133-25ul

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NB100-870

ASC/TMS1 Antibody

(1 Publication)

0.1 mg

NB100-870

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-3078

Alt Catalog# IMG-3078

Conjugate:

Unconjugated

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NBP3-23383

ASC/TMS1 [p Tyr144] Antibody

0.1 ml

NBP3-23383

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PYCARD Products By Gene Name

ASC/TMS1 Antibody - BSA Free

ASC/TMS1 Antibody - BSA Free

ASC/TMS1 Antibody [Unconjugated]

ASC/TMS1 Antibody (1C3D7) - BSA Free

ASC/TMS1 Antibody (OTI3E9)

ASC/TMS1 Antibody (1C3D3) - BSA Free

Recombinant Human ASC/TMS1 GST (N-Term) Protein

ASC/TMS1 Antibody - BSA Free

ASC/TMS1 Antibody - BSA Free

ASC/TMS1 Antibody - BSA Free

ASC/TMS1 Antibody

ASC/TMS1 [p Tyr144] Antibody