Neurolysin Antibody (OTI1D6) - Azide and BSA Free

Images

 
Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NLN mouse monoclonal antibody.
Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN.
Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody.
Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody.
Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Human tonsil using anti-NLN mouse monoclonal antibody.
Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of Jurkat cells, using anti-NLN antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Neurolysin Antibody (OTI1D6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Neurolysin Antibody (OTI1D6) - Azide and BSA Free

  • AGTBP
  • AGTBPFLJ23002
  • angiotensin binding protein
  • Angiotensin-binding protein
  • DKFZp564F123
  • EC 3.4.24
  • EC 3.4.24.16
  • KIAA1226EP24.16
  • MEP
  • mitochondrial
  • MOP
  • neurolysin (metallopeptidase M3 family)
  • Neurolysin
  • Neurotensin Endopeptidase
  • NLN
  • Oligopeptidase M

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NLN