Arginase 1/ARG1/liver Arginase Antibody

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Genetic Strategies: Knockdown Validated: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the ...read more
Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - TRP53-regulated EndMT modulates the M1 and M2 populations of increased TAMs after radiotherapy. Immunofluorescence detection of F4/80, CD31, ...read more
Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Simple Western lane view shows a specific band for ARG1 in human and mouse Liver lysate using ARG1 antibody (NBP1-32731) at 25 ug/ml. This ...read more
Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Effects of berberine on the M2 population of macrophages. Representative dot plot of M2 subpopulations in control group and the rats injected with ...read more
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 antibody detects Arginase 1 protein. Various whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ...read more
Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Analysis was performed using Arginase 1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent. Arginase 1 protein from HepG2 whole ...read more
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton ...read more
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Paraformaldehyde-fixed HeLa.
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Cal27 xenograft, using arginase I antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Tissue section of the mouse liver using 1:200 dilution of ARG1 antibody (NBP1-32731). The signal was developed using HRP-DAB method ...read more
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Hepatocellular carcinoma stained with Arginase1 1:100, pH9 antigen retrieval. Image from verified customer review.
Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 with NBP1-32731 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on RH30 cells with Arginase 1 Antibody NBP1-32731 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Arginase 1/ARG1/liver Arginase Antibody Summary

Immunogen
Arginase 1/ARG1/liver Arginase Antibody made from full length human Arginase 1 Recombinant protein.
Localization
Cytoplasm/Nucleus
Predicted Species
Bovine (90%), Rabbit (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 - 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Simple Western
  • Western Blot 1:1000-1:50000
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32731 in the following applications:

Publications
Read Publications using
NBP1-32731 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32701588). Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (89%). .

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody

  • AI
  • ARG1
  • Arginase 1
  • arginase, liver
  • Arginase-1
  • EC 3.5.3.1
  • EC:3.5.3.1
  • Liver Arginase
  • Liver-type arginase
  • PGIF
  • Type I Arginase

Background

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD

Publications for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731)(10)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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Flow
(2)
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(2)
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(2)
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(1)
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Mouse
(6)
Rat
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Showing Publications 1 - 10 of 10.
Publications using NBP1-32731 Applications Species
Pan X, Zhang K, Shen C et al. Astaxanthin promotes M2 macrophages and attenuates cardiac remodeling after myocardial infarction by suppression inflammation in rats Chin. Med. J. Aug 5 2020 [PMID: 32701588] (Flow, Rat) Flow Rat
Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone Jun 12 2020 [PMID: 32535290] (IHC-P, Mouse) IHC-P Mouse
Cai C, Zhao C, Kilari S et al. Effect of Sex Differences in Treatment Response to Angioplasty in a Murine Arteriovenous Fistula Model Am. J. Physiol. Renal Physiol. Dec 9 2019 [PMID: 31813252]
Che Y, Shen DF, Wang ZP et al. Protective role of berberine in isoprenaline-induced cardiac fibrosis in rats BMC Cardiovasc Disord 2019 Oct 15 [PMID: 31615408] (Flow, Rat) Flow Rat
Gousset K, Gordon A, Kumar Kannan S, Tovar J. A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions Int J Mol Sci Mar 7 2019 [PMID: 30866487] (ICC/IF, Mouse) ICC/IF Mouse
Choi SH, Kim AR, Nam JK, et al. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization. Nat Commun. 2018 Nov 30 [PMID: 30504836] (IHC, WB, Mouse) IHC, WB Mouse
Cao Y, Xu Y, Auchoybur ML et al. Regulatory role of IKKa in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages J. Mol. Cell. Cardiol. Aug 25 2018 [PMID: 30153439] (ICC/IF, Mouse) ICC/IF Mouse
Yang A, Herter-Sprie G, Zhang H, et al. Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms. Cancer Discov. 2018 Jan 09 [PMID: 29317452] (IHC, Mouse) IHC Mouse
Yan J, Tie G, Wang S et al. Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages Nat Commun 2018 Jan 02 [PMID: 29295997] (IHC-Fr, Mouse) IHC-Fr Mouse
Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells SSRN Journal Jun 26 2020

Review for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-32731:
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Images Ratings Applications Species Date Details
 Arginase 1/ARG1/liver Arginase NBP1-32731
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4
reviewed by:
Verified Customer
Human 03/09/2019
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Summary

Sample TestedHepatocellular carcinoma
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 antigen retrieval

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731). (Showing 1 - 1 of 1 FAQs).

  1.  Does the anti-arginase 1 antibody have any specificity towards Arginase II?
    • NBP1-32731 has not been epitope mapped, and cross-reactivity with Arginase II has not been explicitly tested.As you are probably aware, ARG1 and ARG2 share about 60% homology with each other. Since this antibody has not been epitope mapped its hard to say whether this antibody will also detect ARG2. There are some stretches of conservation that are 15 AA long, so it is possible that ARG2 could be recognized with this antibody, especially considering it is a polyclonal antibody. We have 20 primary antibodies that target ARG1, so perhaps you could find one that has the immunogen listed:https://www.novusbio.com/search?keywords=arginase%201&category=Primary%20Antibodies&common_name=Arginase%201/ARG1/liver%20Arginase

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-32731AF350
Alexa Fluor 405 NBP1-32731AF405
Alexa Fluor 488 NBP1-32731AF488
Alexa Fluor 532 NBP1-32731AF532
Alexa Fluor 594 NBP1-32731AF594
Alexa Fluor 647 NBP1-32731AF647
Alexa Fluor 700 NBP1-32731AF700
Alexa Fluor 750 NBP1-32731AF750
Allophycocyanin NBP1-32731APC
Biotin NBP1-32731B
DyLight 350 NBP1-32731UV
DyLight 405 NBP1-32731V
DyLight 488 NBP1-32731G
DyLight 550 NBP1-32731R
DyLight 594 NBP1-32731DL594
DyLight 650 NBP1-32731C
DyLight 680 NBP1-32731FR
DyLight 755 NBP1-32731IR
FITC NBP1-32731F
HRP NBP1-32731H
Janelia Fluor 549 NBP1-32731JF549
Janelia Fluor 646 NBP1-32731JF646
PE NBP1-32731PE
PerCP NBP1-32731PCP

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Verified Customer
03/09/2019
Application:
Species: Human

Bioinformatics

Gene Symbol ARG1
Entrez
Uniprot