Genetic Strategies: Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane ...read more
Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - HepG2 cell. Black: Unlabelled sample was used as a control. Red: Arginase 1 antibody dilution: 1:50. Acquisition of 20,000 events were collected ...read more
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 antibody detects Arginase 1 protein by western blot analysis. Various whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the ...read more
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - paraffin-embedded Cal27 xenograft, using arginase I antibody at 1:500 dilution.
Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 protein from HepG2 whole cell extracts using 5 ug of Arginase 1 antibody. Western blot analysis was performed using Arginase 1 ...read more
35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-32731 in the following applications:
Expected cross reactivity based on sequence homology: Porcine (89%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Arginase 1/ARG1/liver Arginase Antibody
Type I Arginase
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic cross reactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Arginase 1/ARG1/liver Arginase Antibody (NBP1-32731). (Showing 1 - 1 of 1 FAQ).
Does the anti-arginase 1 antibody have any specificity towards Arginase II?
NBP1-32731 has not been epitope mapped, and cross-reactivity with Arginase II has not been explicitly tested.As you are probably aware, ARG1 and ARG2 share about 60% homology with each other. Since this antibody has not been epitope mapped its hard to say whether this antibody will also detect ARG2. There are some stretches of conservation that are 15 AA long, so it is possible that ARG2 could be recognized with this antibody, especially considering it is a polyclonal antibody. We have 20 primary antibodies that target ARG1, so perhaps you could find one that has the immunogen listed:https://www.novusbio.com/search?keywords=arginase%201&category=Primary%20Antibodies&common_name=Arginase%201/ARG1/liver%20Arginase
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