STAT6 Antibody (177C322.1)

Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.

Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clone: 177C322.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

STAT6 Antibody (177C322.1) Summary

• Immunogen: A portion of amino acids 600-650 of human Signal Transducer and Activator of Transcription 6 was used as the immunogen for the STAT6 Antibody (177C322.1).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunoprecipitation 1-2 ug/ml
• Control:

  STAT6 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for STAT6 Antibody (177C322.1)

• D12S1644
• EC 2.4.1.227
• EC 2.7.7.6
• IL-4 Stat
• IL-4-STAT
• signal transducer and activator of transcription 6
• signal transducer and activator of transcription 6, interleukin-4 induced
• STAT, interleukin-4 induced
• STAT, interleukin4-induced
• STAT6
• STAT6B
• STAT6C
• transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13(1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response(2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References
1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for STAT6 Antibody (NBP2-25241). (Showing 1 - 1 of 1 FAQ).

1. Has there been any specificity testing performed with the Monoclonal Antibody to phospho-STAT6 (Tyr641) Cat. No. NBP2-25241? The results of a preliminary experiment indicate that NBP2-25241 may react with full length STAT6 in the researcher's hands.
   • The antibody is tested on NIH3T3 cells. I would imagine that the antibody would react with full length recombinant STAT6 since the antibody is made against STAT6. Phosphospecific antibodies have preferences for the phosphoforms. However when there are large amounts of non-phosphorylated protein present such as recombinant protein they will often react with them.

Bioinformatics

• Gene Symbol: STAT6
• Uniprot:
  • NP_003144()