STAT6 Antibody (177C322.1)

Images

 
Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
177C322.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

STAT6 Antibody (177C322.1) Summary

Immunogen
A portion of amino acids 600-650 of human Signal Transducer and Activator of Transcription 6 was used as the immunogen for the STAT6 Antibody (177C322.1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunoprecipitation 1-2 ug/ml
Control
STAT6 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP2-25241 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for STAT6 Antibody (177C322.1)

  • D12S1644
  • EC 2.4.1.227
  • EC 2.7.7.6
  • IL-4 Stat
  • IL-4-STAT
  • signal transducer and activator of transcription 6
  • signal transducer and activator of transcription 6, interleukin-4 induced
  • STAT, interleukin-4 induced
  • STAT, interleukin4-induced
  • STAT6
  • STAT6B
  • STAT6C
  • transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT6 Antibody (NBP2-25241)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAT6 Antibody (NBP2-25241). (Showing 1 - 1 of 1 FAQ).

  1. Has there been any specificity testing performed with the Monoclonal Antibody to phospho-STAT6 (Tyr641) Cat. No. NBP2-25241? The results of a preliminary experiment indicate that NBP2-25241 may react with full length STAT6 in the researcher's hands.
    • The antibody is tested on NIH3T3 cells. I would imagine that the antibody would react with full length recombinant STAT6 since the antibody is made against STAT6. Phosphospecific antibodies have preferences for the phosphoforms. However when there are large amounts of non-phosphorylated protein present such as recombinant protein they will often react with them.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25241AF350
Alexa Fluor 405 NBP2-25241AF405
Alexa Fluor 488 NBP2-25241AF488
Alexa Fluor 532 NBP2-25241AF532
Alexa Fluor 594 NBP2-25241AF594
Alexa Fluor 647 NBP2-25241AF647
Alexa Fluor 700 NBP2-25241AF700
Alexa Fluor 750 NBP2-25241AF750
Biotin NBP2-25241B
DyLight 350 NBP2-25241UV
DyLight 405 NBP2-25241V
DyLight 488 NBP2-25241G
DyLight 550 NBP2-25241R
DyLight 594 NBP2-25241DL594
DyLight 650 NBP2-25241C
DyLight 680 NBP2-25241FR
DyLight 755 NBP2-25241IR
HRP NBP2-25241H
Janelia Fluor 549 NBP2-25241JF549
Janelia Fluor 646 NBP2-25241JF646

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Bioinformatics Tool for STAT6 Antibody (NBP2-25241)

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Blogs on STAT6.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Bioinformatics

Gene Symbol STAT6
Uniprot