Arginase 1/ARG1/liver Arginase Antibodies: Novus Biologicals

NBP1-32731

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

(1 Review)

(30 Publications)

0.1 mg

NBP1-32731

Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Simple Western lane view shows a specific band for ARG1 in human and mouse Liver lysate using ARG1 antibody (NBP1-32731) at 25 ug/ml. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Arginase-1-ARG1-liver-Arginase-Antibody-Flow-Cytometry-NBP1-32731-img0032.jpg

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Arginase 1 antibody detects Arginase 1 protein. Various whole cell extracts (30ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:1000.

Immunoprecipitation: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Analysis was performed using Arginase 1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent. Arginase 1 protein from HepG2 whole cell extracts using 5 ug of Arginase 1 antibody.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 cells with Arginase 1/ARG1/liver Arginase Antibody NBP1-32731AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Paraformaldehyde-fixed HeLa.

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ARG1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Cal27 xenograft, using arginase I antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Tissue section of the mouse liver using 1:200 dilution of ARG1 antibody (NBP1-32731). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - Hepatocellular carcinoma stained with Arginase1 1:100, pH9 antigen retrieval. Image from verified customer review.

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on HepG2 with NBP1-32731 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-32731] - An intracellular stain was performed on RH30 cells with Arginase 1 Antibody NBP1-32731 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

nbp1-32731_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-862023835350.jpg

Effects of berberine on the M2 population of macrophages. Representative dot plot of M2 subpopulations in control group and the rats injected with ISO 3 and 7 days later. M2 cells were labelled with F4/80 and Arg1. *P < 0.05 as compared with the control group. #P < 0.05 vs. the corresponding ISO group

Immunocytochemistry/ Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody - BSA Free [NBP1-32731] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS (A–D) “expected” or (E–H) “not expected” to be within protrusions based on their known functions/localizations. (A) Cd47 & (B) Anxa2 are found in both hCAD/dCAD protrusions. (C) Tenm2 is found within hCAD protrusions & (D) Cobl in GCs. Interestingly, (E) Grk5 was observed in hCAD protrusions; (F) Hist1h3b protein in hCAD protrusions & GCs (G) Hspa1b in dCAD protrusions & TNTs & (H) Arg1 in dCAD protrusions, GCs, & TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions & GCs or within TNTs, respectively. Scale bars = 10 μm. IF of all 8 proteins corroborate the LCM/MS protein identification for each subtypes of protrusions. (I) 1198 unique proteins were identified by LCM/MS to be in protrusions & 169 were found to be exclusive to protrusions. 904 of the 1198 proteins found in protrusions & 87 out of 169 exclusive proteins had images in the HPA Subcell database. IF images for the proteins identified by LCM/MS in protrusions, present in the HPA Subcell database, were observed & catalogued as being observed or not in protrusions (Figure S2). On average 4.7% of proteins are found in protrusions in the HPA database, 13.6% are found in our LCM isolated protrusions & 23% were found in the “exclusive” LCM protrusions. The fold enrichment increases by 2.9% or 4.8% when we looked at proteins identified by LCM/MS in protrusions or exclusive to protrusions, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Arginase 1/ARG1/liver Arginase was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti-Arginase 1/ARG1/liver Arginase Affinity Purified Polyclonal Antibody conjugated to FITC (Catalog # NBP1-32731F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Rb

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-32731AF350
Alexa Fluor 405	NBP1-32731AF405
Alexa Fluor 488	NBP1-32731AF488
Alexa Fluor 532	NBP1-32731AF532
Alexa Fluor 594	NBP1-32731AF594
Alexa Fluor 647	NBP1-32731AF647
Alexa Fluor 700	NBP1-32731AF700
Alexa Fluor 750	NBP1-32731AF750
Alexa Fluor Plus 405	NBP1-32731AFP405
Alexa Fluor Plus 488	NBP1-32731AFP488
Alexa Fluor Plus 555	NBP1-32731AFP555
Alexa Fluor Plus 594	NBP1-32731AFP594
Alexa Fluor Plus 647	NBP1-32731AFP647
Alexa Fluor Plus 680	NBP1-32731AFP680
Allophycocyanin	NBP1-32731APC
Biotin	NBP1-32731B
CoraFluor 1	NBP1-32731CL1
DyLight 350	NBP1-32731UV
DyLight 405	NBP1-32731V
DyLight 488	NBP1-32731G
DyLight 550	NBP1-32731R
DyLight 594	NBP1-32731DL594
DyLight 650	NBP1-32731C
DyLight 680	NBP1-32731FR
DyLight 755	NBP1-32731IR
FITC	NBP1-32731F
HRP	NBP1-32731H
Janelia Fluor 525	NBP1-32731JF525
Janelia Fluor 549	NBP1-32731JF549
Janelia Fluor 646	NBP1-32731JF646
Janelia Fluor 669	NBP1-32731JF669
PE	NBP1-32731PE
PerCP	NBP1-32731PCP
mFluor Violet 450 SE	NBP1-32731MFV450
mFluor Violet 500 SE	NBP1-32731MFV500
mFluor Violet 610 SE	NBP1-32731MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-32731SS

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NB100-59740

Arginase 1/ARG1/liver Arginase Antibody

(20 Publications)

0.1 mg

NB100-59740

Under IL-10 treatment, PC1/3 KD cells exhibit lower levels of arginase than NT cells. NT and PC1/3 KD NR8383 macrophages were treated with 20 ng/mL of IL-10 for 24 h. (A) Western blot studies conducted with anti-iNOS. (B) Intensities of iNOS quantified and normalized to those of Actin. (C) Intensities of s-iNOS quantified and normalized to those of Actin. (D) Western blot studies conducted with anti-arginase. (E) Intensities of arginase quantified and normalized to those of Actin. The results are obtained from three independent experiments and depicted through graphic representations (means +/- SD). Data are analyzed by the Student t-test and values of p < 0.05 are considered statistically significant (*p-value of <0.05, **p-value of <0.01). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31766635), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western blot results. (A) Representative Western blot and quantitative analysis of protein levels of Iba‐1, iNOS, ARG1, IL‐1 beta , and TNF‐ alpha in PFC tissues, n = 6. (B) Relative level of Iba‐1 protein in PFC (fold change relative to beta ‐actin protein level). (C) The relative level of iNOS protein in PFC (fold change relative to beta ‐actin protein level). (D) The relative level of ARG1 protein in PFC (fold change relative to beta ‐actin protein level). (E) The relative level of IL‐1 beta protein in PFC (fold change relative to beta ‐actin protein level). (F) The relative level of TNF‐ alpha protein in PFC (fold change relative to beta ‐actin protein level). *p < 0.05 versus Con group. ARG1, arginase‐1; IL‐1 beta , interleukin‐1 beta ; iNOS, inducible nitric oxide synthase; PFC, prefrontal cortex; TNF‐ alpha , tumor necrosis factor‐ alpha [Color figure can be viewed at wileyonlinelibrary.com] Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37786559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-30305

Alt Catalog# IMG-30305

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF5868

Arginase 1/ARG1/liver Arginase Antibody [Unconjugated]

(17 Publications)

100 μg

AF5868

HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

<b>Biological Strategies Validation. </b>Sepsis induces polarization of M2-like macrophages.(a–e) Lung tissue and peritoneal cells from C57BL/6 J (a,c,d) and BALB/c (b,e) mice undergoing CLP and antibiotic treatment were harvested at the indicated time points. (a) mRNA expression of Cebpb (C/EBP beta ), Arg1 (arginase-1), Mrc1 (MR) and Igf1 in the total lungs were determined by RT-qPCR at indicated times after CLP (n≥4 mice per group). (b) Lungs obtained from six mice, either naive or CLP (day 10 after CLP), were pooled from two independent samples and CD11b+ cells were isolated. mRNA expression of Arg1 (arginase-1), Mrc1 (MR), Rentla (encoding Fizz1) in isolated CD11b+ cells were determined by RT-qPCR. (c) IGF-1, CCL17 and CCL22 concentrations in the lungs were determined by ELISA at indicated times after CLP (n≥3 mice per group). (d) Representative FACS plots and frequency of peritoneal CD206+F4/80+ macrophages at indicated times after CLP (n≥3 mice per group). (e) Representative western blot of Arg1 (Arginase-1) expression in the peritoneal cells at day 10 after CLP (n=9 for naive group and n=5 for CLP group). ND, not detected. *P<0.05, **P<0.01 and ***P<0.001 (one-way ANOVA result with Dunnett posthoc tests in a,c, two-tailed unpaired Student's t-test in b,d). Data are from one (b) and representative of two (a,c–e) independent experiments (mean±s.e.m. in a–d). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28374774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5868U-100UG
Alexa Fluor 405	AF5868V-100UG
Alexa Fluor 488	AF5868G-100UG
Alexa Fluor 532	AF5868X-100UG
Alexa Fluor 594	AF5868T-100UG
Alexa Fluor 647	AF5868R-100UG
Alexa Fluor 700	IC5868N-100UG
Alexa Fluor 750	AF5868S-100UG
Alexa Fluor Plus 405	AF5868AFP405-100UG
Alexa Fluor Plus 488	AF5868AFP488-100UG
Alexa Fluor Plus 555	AF5868AFP555-100UG
Alexa Fluor Plus 594	AF5868AFP594-100UG
Alexa Fluor Plus 647	AF5868AFP647-100UG
Alexa Fluor Plus 680	AF5868AFP680-100UG
Allophycocyanin	IC5868A
Biotin	AF5868B
CoraFluor 1	AF5868CL1
DyLight 350	AF5868D
DyLight 405	AF5868E
DyLight 488	AF5868K
DyLight 550	AF5868L
DyLight 594	AF5868M
DyLight 650	AF5868W
DyLight 680	AF5868Y
DyLight 755	AF5868Z
Fluorescein	IC5868F
HRP	AF5868H
Janelia Fluor 525	AF5868JF525
Janelia Fluor 549	AF5868I
Janelia Fluor 646	AF5868J
Janelia Fluor 669	AF5868JF669
PerCP	AF5868C
Phycoerythrin	IC5868P
mFluor Violet 450 SE	AF5868MFV450
mFluor Violet 500 SE	AF5868MFV500
mFluor Violet 610 SE	AF5868MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC5868F
Carrier Free	IC5868P
Carrier Free	IC5868A
Carrier Free	AF5868-SP
Carrier Free	IC5868N-100UG

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NBP1-54621

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

(8 Publications)

100 ul

NBP1-54621

nbp1-54621_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-20920231412575.jpg

A, Anti–arginase-1 (Arg1) immunohistochemistry (IHC) staining results (n = 4-5; ∗P = .0210) and representative slides. B, Anti-DDR2 IHC staining results with negative antibody controls included. Original magnification ×1000 for all representative images (oil immersion). C, Anti-CD31 IHC staining analysis results. Original magnification ×400 (n = 4-5 for all stains).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-87490

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

(6 Publications)

0.1 ml

NBP1-87490

<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Analysis using Anti-ARG1 antibody NBP1-87490 (A) shows similar pattern to independent antibody NBP1-87455 (B).

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human hepatocellular carcinoma shows moderate cytoplasmic and nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:50 dilution on Liver and HepG2 lysate(s).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87490-25ul

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NBP2-03618

Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)

(5 Publications)

0.1 ml

NBP2-03618

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-liver Arginase antibody, and then analyzed by flow cytometry.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver Arginase.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70204AF350
Alexa Fluor 405	NBP2-70204AF405
Alexa Fluor 488	NBP2-70204AF488
Alexa Fluor 532	NBP2-70204AF532
Alexa Fluor 594	NBP2-70204AF594
Alexa Fluor 647	NBP2-70204AF647
Alexa Fluor 700	NBP2-70204AF700
Alexa Fluor 750	NBP2-70204AF750
Alexa Fluor Plus 405	NBP2-70204AFP405
Alexa Fluor Plus 488	NBP2-70204AFP488
Alexa Fluor Plus 555	NBP2-70204AFP555
Alexa Fluor Plus 594	NBP2-70204AFP594
Alexa Fluor Plus 647	NBP2-70204AFP647
Alexa Fluor Plus 680	NBP2-70204AFP680
Allophycocyanin	NBP2-70204APC
Biotin	NBP2-70204B
CoraFluor 1	NBP2-70204CL1
DyLight 350	NBP2-70204UV
DyLight 405	NBP2-70204V
DyLight 488	NBP2-70204G
DyLight 550	NBP2-70204R
DyLight 594	NBP2-70204DL594
DyLight 650	NBP2-70204C
DyLight 680	NBP2-70204FR
DyLight 755	NBP2-70204IR
FITC	NBP2-70204F
HRP	NBP2-70204H
Janelia Fluor 525	NBP2-70204JF525
Janelia Fluor 549	NBP2-70204JF549
Janelia Fluor 646	NBP2-70204JF646
Janelia Fluor 669	NBP2-70204JF669
PE	NBP2-70204PE
PerCP	NBP2-70204PCP
mFluor Violet 450 SE	NBP2-70204MFV450
mFluor Violet 500 SE	NBP2-70204MFV500
mFluor Violet 610 SE	NBP2-70204MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70204

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MAB5868

Arginase 1/ARG1/liver Arginase Antibody (658934) [Unconjugated]

(1 Publication)

100 μg

MAB5868

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." class="big_lightbox">

Arginase 1 was detected in immersion fixed paraffin-embedded sections of human Liver Tumor using Mouse Anti-Human Arginase 1, Monoclonal Antibody (Catalog # MAB5868) at 3ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." alt="Arginase 1 was detected in immersion fixed paraffin-embedded sections of human Liver Tumor using Mouse Anti-Human Arginase 1, Monoclonal Antibody (Catalog # MAB5868) at 3ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF007). A specific band was detected for Arginase 1/ARG1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human Arginase 1/ARG1 Monoclonal Antibody (Catalog # MAB5868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Arginase 1/ARG1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Arginase 1/ARG1 Monoclonal Antibody (Catalog # MAB5868). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.</PP>*Non-specific interaction with the 230kDa Simple Western standard may be seen with this antibody.

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Arginase 1/ARG1 was detected in immersion fixed paraffin-embedded sections of human liver cancer using Mouse Anti-Human Arginase 1/ARG1 Monoclonal Antibody (Catalog # MAB5868) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Hypoxic glioma cell‐derived EVs promote M2 macrophage polarization. (A) Images of EVs observed under a TEM (Scale bar = 100 nm). (B) Diameter of EVs detected by dynamic light scattering. (C) The expression of CD9, CD63, TSG101, and calnexin on the surface of EVs determined by Immunoblotting. (D) Detection of the uptake of EVs from normoxic and hypoxic glioma cells by macrophages U937 through immunofluorescence (400×). (E) The mRNA expression of iNOS, TNF‐ alpha , Arg‐1, and IL‐10 assessed by RT‐qPCR after EV treatment. (F) Immunoblotting of protein expression of iNOS and Arg‐1 in macrophages U937 after EV treatment. (G) The expression of TNF‐ alpha and IL‐10 in supernatant of macrophages U937 evaluated by ELISA assay. (H) The proportion of CD11b+CD163+ cells in U937 cells examined by flow cytometry. *p < 0.05 versus macrophages treated with PBS. #p < 0.05 versus macrophages treated with normal oxygen‐induced glioma cell‐derived EVs. The experiment was repeated three times Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36052751), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5868U-100UG
Alexa Fluor 405	FAB5868V-100UG
Alexa Fluor 488	FAB5868G-100UG
Alexa Fluor 532	FAB5868X-100UG
Alexa Fluor 594	FAB5868T-100UG
Alexa Fluor 647	FAB5868R-100UG
Alexa Fluor 700	FAB5868N-100UG
Alexa Fluor 750	FAB5868S-100UG
Alexa Fluor Plus 405	FAB5868AFP405-100UG
Alexa Fluor Plus 488	FAB5868AFP488-100UG
Alexa Fluor Plus 555	FAB5868AFP555-100UG
Alexa Fluor Plus 594	FAB5868AFP594-100UG
Alexa Fluor Plus 647	FAB5868AFP647-100UG
Alexa Fluor Plus 680	FAB5868AFP680-100UG
Allophycocyanin	FAB5868A
Biotin	FAB5868B
CoraFluor 1	FAB5868CL1
DyLight 350	FAB5868D
DyLight 405	FAB5868E
DyLight 488	FAB5868K
DyLight 550	FAB5868L
DyLight 594	FAB5868M
DyLight 650	FAB5868W
DyLight 680	FAB5868Y
DyLight 755	FAB5868Z
FITC	FAB5868F
HRP	FAB5868H
Janelia Fluor 525	FAB5868JF525
Janelia Fluor 549	FAB5868I
Janelia Fluor 646	FAB5868J
Janelia Fluor 669	FAB5868JF669
PE	FAB5868P
PerCP	FAB5868C
mFluor Violet 450 SE	FAB5868MFV450
mFluor Violet 500 SE	FAB5868MFV500
mFluor Violet 610 SE	FAB5868MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5868-SP

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NBP1-36936

Arginase 1/ARG1/liver Arginase Antibody

0.1 mg

NBP1-36936

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-30930

Alt Catalog# IMG-30930

Conjugate:

Unconjugated

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NBP2-14787

Arginase 1/ARG1/liver Arginase Antibody (CL0186) - BSA Free

0.1 ml

NBP2-14787

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Lane 1: Marker [kDa], Lane 2: Human liver tissue lysate

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Staining of human prostate shows no immunoreactivity (negative control).

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.

Simple Western: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:20 dilution on Liver lysate(s).

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (CL0186) [NBP2-14787] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44441AF350
Alexa Fluor 405	NBP3-44441AF405
Alexa Fluor 488	NBP3-44441AF488
Alexa Fluor 532	NBP3-44441AF532
Alexa Fluor 594	NBP3-44441AF594
Alexa Fluor 647	NBP3-44441AF647
Alexa Fluor 700	NBP3-44441AF700
Alexa Fluor 750	NBP3-44441AF750
Alexa Fluor Plus 405	NBP3-44441AFP405
Alexa Fluor Plus 488	NBP3-44441AFP488
Alexa Fluor Plus 555	NBP3-44441AFP555
Alexa Fluor Plus 594	NBP3-44441AFP594
Alexa Fluor Plus 647	NBP3-44441AFP647
Alexa Fluor Plus 680	NBP3-44441AFP680
Allophycocyanin	NBP3-44441APC
Biotin	NBP3-44441B
CoraFluor 1	NBP3-44441CL1
DyLight 350	NBP3-44441UV
DyLight 405	NBP3-44441V
DyLight 488	NBP3-44441G
DyLight 550	NBP3-44441R
DyLight 594	NBP3-44441DL594
DyLight 650	NBP3-44441C
DyLight 680	NBP3-44441FR
DyLight 755	NBP3-44441IR
FITC	NBP3-44441F
HRP	NBP3-44441H
Janelia Fluor 525	NBP3-44441JF525
Janelia Fluor 549	NBP3-44441JF549
Janelia Fluor 646	NBP3-44441JF646
Janelia Fluor 669	NBP3-44441JF669
PE	NBP3-44441PE
PerCP	NBP3-44441PCP
mFluor Violet 450 SE	NBP3-44441MFV450
mFluor Violet 500 SE	NBP3-44441MFV500
mFluor Violet 610 SE	NBP3-44441MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44441
BSA Free	NBP2-14787-25ul

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NBP1-87455

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-87455

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining in human liver and pancreas tissues using anti-ARG1 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human liver shows high expression.

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human pancreas shows low expression as expected.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87455-25ul

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NBP2-89915

Arginase 1/ARG1/liver Arginase Antibody (034) - Azide and BSA Free

100 ul

NBP2-89915

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000).

Immunocytochemistry/Immunofluorescence: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89915 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (034) [NBP2-89915] - Analysis of Human ARG1 expression on HepG2 cells. The cells were treated, stained with NBP2-89915, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89915AF350
Alexa Fluor 405	NBP2-89915AF405
Alexa Fluor 488	NBP2-89915AF488
Alexa Fluor 532	NBP2-89915AF532
Alexa Fluor 594	NBP2-89915AF594
Alexa Fluor 647	NBP2-89915AF647
Alexa Fluor 700	NBP2-89915AF700
Alexa Fluor 750	NBP2-89915AF750
Alexa Fluor Plus 405	NBP2-89915AFP405
Alexa Fluor Plus 488	NBP2-89915AFP488
Alexa Fluor Plus 555	NBP2-89915AFP555
Alexa Fluor Plus 594	NBP2-89915AFP594
Alexa Fluor Plus 647	NBP2-89915AFP647
Alexa Fluor Plus 680	NBP2-89915AFP680
Allophycocyanin	NBP2-89915APC
Biotin	NBP2-89915B
CoraFluor 1	NBP2-89915CL1
DyLight 350	NBP2-89915UV
DyLight 405	NBP2-89915V
DyLight 488	NBP2-89915G
DyLight 550	NBP2-89915R
DyLight 594	NBP2-89915DL594
DyLight 650	NBP2-89915C
DyLight 680	NBP2-89915FR
DyLight 755	NBP2-89915IR
FITC	NBP2-89915F
HRP	NBP2-89915H
Janelia Fluor 525	NBP2-89915JF525
Janelia Fluor 549	NBP2-89915JF549
Janelia Fluor 646	NBP2-89915JF646
Janelia Fluor 669	NBP2-89915JF669
PE	NBP2-89915PE
PerCP	NBP2-89915PCP
mFluor Violet 450 SE	NBP2-89915MFV450
mFluor Violet 500 SE	NBP2-89915MFV500
mFluor Violet 610 SE	NBP2-89915MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89915-50ul

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NBP2-44965

Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125)

0.1 mg

NBP2-44965

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).

Protein Array: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47998AF350
Alexa Fluor 405	NBP2-47998AF405
Alexa Fluor 488	NBP2-47998AF488
Alexa Fluor 532	NBP2-47998AF532
Alexa Fluor 594	NBP2-47998AF594
Alexa Fluor 647	NBP2-47998AF647
Alexa Fluor 700	NBP2-47998AF700
Alexa Fluor 750	NBP2-47998AF750
Alexa Fluor Plus 405	NBP2-47998AFP405
Alexa Fluor Plus 488	NBP2-47998AFP488
Alexa Fluor Plus 555	NBP2-47998AFP555
Alexa Fluor Plus 594	NBP2-47998AFP594
Alexa Fluor Plus 647	NBP2-47998AFP647
Alexa Fluor Plus 680	NBP2-47998AFP680
Allophycocyanin	NBP2-47998APC
Biotin	NBP2-47998B
CoraFluor 1	NBP2-47998CL1
DyLight 350	NBP2-47998UV
DyLight 405	NBP2-47998V
DyLight 488	NBP2-47998G
DyLight 550	NBP2-47998R
DyLight 594	NBP2-47998DL594
DyLight 650	NBP2-47998C
DyLight 680	NBP2-47998FR
DyLight 755	NBP2-47998IR
FITC	NBP2-47998F
HRP	NBP2-47998H
Janelia Fluor 525	NBP2-47998JF525
Janelia Fluor 549	NBP2-47998JF549
Janelia Fluor 646	NBP2-47998JF646
Janelia Fluor 669	NBP2-47998JF669
PE	NBP2-47998PE
mFluor Violet 450 SE	NBP2-47998MFV450
mFluor Violet 500 SE	NBP2-47998MFV500
mFluor Violet 610 SE	NBP2-47998MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47998-0.1mg
IHC-Prediluted	NBP2-48229

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Arginase 1/ARG1/Liver Arginase Antibodies

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Arginase 1/ARG1/liver Arginase Antibody

Arginase 1/ARG1/liver Arginase Antibody [Unconjugated]

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Arginase 1/ARG1/liver Arginase Antibody (OTI4E6)

Arginase 1/ARG1/liver Arginase Antibody (658934) [Unconjugated]

Arginase 1/ARG1/liver Arginase Antibody

Arginase 1/ARG1/liver Arginase Antibody (CL0186) - BSA Free

Arginase 1/ARG1/liver Arginase Antibody - BSA Free

Arginase 1/ARG1/liver Arginase Antibody (034) - Azide and BSA Free

Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125)