ARG2 Antibody (OTI3G5)

Western Blot: ARG2 Antibody (OTI3G5) [NBP2-46539] - Analysis of extracts (10ug) from 3 different mouse tissues, dilution (1:200).

Immunocytochemistry/Immunofluorescence: ARG2 Antibody (OTI3G5) [NBP2-46539] - ARG2 Antibody (3G5) [NBP2-46539] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ARG2.

Immunohistochemistry: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARG2.

Western Blot: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: ARG2 Antibody (3G5) [NBP2-46539] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Rt, Ca
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ARG2 Antibody (OTI3G5) Summary

• Immunogen: Full length human recombinant protein of human ARG2(NP_001163) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:400-4000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARG2 Antibody (OTI3G5)

• ARG2
• Arginase 2
• arginase, type II
• Arginase-2
• arginase-2, mitochondrial
• EC 3.5.3
• EC 3.5.3.1
• kidney arginase
• Kidney-type arginase
• L-arginine amidinohydrolase
• L-arginine ureahydrolase
• nonhepatic arginase
• Non-hepatic arginase
• Type II Arginase

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARG2