AMPK alpha 2 Antibody (S06-2E8)

Western Blot: AMPK alpha 2 Antibody (S06-2E8) [NBP3-19569] - Western blot detection of AMPK alpha 2 in C6, Hela cell lysates using NBP3-19569 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 62kDa.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: S06-2E8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

AMPK alpha 2 Antibody (S06-2E8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human AMPK alpha 2 (Uniprot # P54646)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKAA2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for AMPK alpha 2 Antibody (S06-2E8)

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAA2