LKB1/STK11 Antibody

Images

 
Western Blot: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in human testes lysate.
Immunocytochemistry/ Immunofluorescence: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.2 mg/ml

LKB1/STK11 Antibody Summary

Immunogen
A synthetic peptide made to a internal portion of the human LKB1 protein (between residues 250-350) [UniProt Q15831]
Localization
The inactive form localizes to the nucleus; upon activation LKB1 translocates to the cytoplasm and mitochondria.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This LKB1 antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections and Western blot. In Western blot a band is detected ~48 kDa in human testes. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse stomach. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, nuclear staining was observed in HepG2 cells.
Control
LKB1/STK11 Knockout 293T Cell Lysate
Publications
Read Publication using
NBP2-14835 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Immunogen has 100% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LKB1/STK11 Antibody

  • EC 2.7.11.1
  • LKB1 serine/threonine kinase 11 (Peutz-Jeghers syndrome)
  • LKB1
  • PJS polarization-related protein LKB1
  • PJS
  • Renal carcinoma antigen NY-REN-19
  • serine/threonine kinase 11
  • serine/threonine-protein kinase 11
  • Serine/threonine-protein kinase LKB1
  • STK11

Background

Liver kinase B1 (LKB1), also known as serine/threonine-protein kinase STK11, is a member of the serine/threonine kinase family. LKB1 is a tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. More specifically, LKB1 controls the activity of AMP-activated protein kinase (AMPK) family members, regulates cell polarity by remodeling the actin cytoskeleton, regulates the inhibition of cell growth signaling pathways in response to low energy levels, regulates autophagy in response to nutrient deprivation, and is involved in DNA damage response by differentiating B-cells in the germinal center. LKB1 phosphorylation in cells occurs at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363. LKB1 is ubiquitously expressed in tissues with the strongest expression in testis and fetal liver. Defects in the LKB1 gene have been known to cause Peutz-Jeghers syndrome (PJS), testicular germ cell tumors (TGCT), non-small cell lung cancer (NSCLC), and other sporadic cancers. While not thoroughly characterized, alternate transcriptional splice variants of the LKB1 gene have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LKB1/STK11 Antibody (NBP2-14835)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LKB1/STK11 Antibody (NBP2-14835) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-14835AF350
Alexa Fluor 405 NBP2-14835AF405
Alexa Fluor 488 NBP2-14835AF488
Alexa Fluor 532 NBP2-14835AF532
Alexa Fluor 594 NBP2-14835AF594
Alexa Fluor 647 NBP2-14835AF647
Alexa Fluor 700 NBP2-14835AF700
Alexa Fluor 750 NBP2-14835AF750
Biotin NBP2-14835B
DyLight 350 NBP2-14835UV
DyLight 405 NBP2-14835V
DyLight 488 NBP2-14835G
DyLight 550 NBP2-14835R
DyLight 594 NBP2-14835DL594
DyLight 650 NBP2-14835C
DyLight 680 NBP2-14835FR
DyLight 755 NBP2-14835IR
FITC NBP2-14835F
HRP NBP2-14835H
Janelia Fluor 549 NBP2-14835JF549
Janelia Fluor 646 NBP2-14835JF646

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Bioinformatics

Gene Symbol STK11
Entrez
Uniprot