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AMPK alpha 1 Antibody (2B7) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2B7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22127AF350
Alexa Fluor 405
NBP2-22127AF405
Alexa Fluor 488
NBP2-22127AF488
Alexa Fluor 532
NBP2-22127AF532
Alexa Fluor 594
NBP2-22127AF594
Alexa Fluor 647
NBP2-22127AF647
Alexa Fluor 700
NBP2-22127AF700
Alexa Fluor 750
NBP2-22127AF750
Allophycocyanin
NBP2-22127APC
Allophycocyanin/Cy7
NBP2-22127APCCY7
Biotin
NBP2-22127B
DyLight 350
NBP2-22127UV
DyLight 488
NBP2-22127G
DyLight 550
NBP2-22127R
DyLight 594
NBP2-22127DL594
DyLight 650
NBP2-22127C
DyLight 680
NBP2-22127FR
DyLight 755
NBP2-22127IR
FITC
NBP2-22127F
HRP
NBP2-22127H
Janelia Fluor 549
NBP2-22127JF549
Janelia Fluor 646
NBP2-22127JF646
PE
NBP2-22127PE
PE/Atto594
NBP2-22127PEATT594
PE/Cy5.5
NBP2-22127PECY55
PE/Cy7
NBP2-22127PECY7
PerCP
NBP2-22127PCP
Unconjugated
NBP2-22127
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22127
Azide and BSA Free
NBP2-80575
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AMPK alpha 1 Antibody (2B7) [DyLight 405] Summary

Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for AMPK alpha 1 Antibody (2B7) [DyLight 405]

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
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Immunohistochemistry-Paraffin: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.
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Immunocytochemistry/Immunofluorescence: ATIC Antibody [NBP1-89192] - Staining of human cell line A-431 shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATIC Antibody [NBP1-89192] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Adiponectin/Acrp30
[HRP]
DRP300
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Carboxylase alpha/ACA ...
NBP2-89114
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eNOS Antibody
NB300-500
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Antibody [Unconjugate
AF2850
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Ribosomal Protein
S6/RPS6 Antibody
NB100-1595
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Synthase/FASN Antibod
NB400-114
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Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
(2 Reviews)
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Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
PYK2/FAK2 Antibody
(SC06-15)
NBP2-67627
Western Blot: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1:1000 dilution.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AMPK alpha 1
Antibody (2B7) [DyLig ...
NBP2-22127V
AMPK alpha 1 Antibody (2B7) [DyLight 405]
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMPK alpha 1 Antibody (NBP2-22127V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22127AF350
Alexa Fluor 405 NBP2-22127AF405
Alexa Fluor 488 NBP2-22127AF488
Alexa Fluor 532 NBP2-22127AF532
Alexa Fluor 594 NBP2-22127AF594
Alexa Fluor 647 NBP2-22127AF647
Alexa Fluor 700 NBP2-22127AF700
Alexa Fluor 750 NBP2-22127AF750
Allophycocyanin NBP2-22127APC
Allophycocyanin/Cy7 NBP2-22127APCCY7
Biotin NBP2-22127B
DyLight 350 NBP2-22127UV
DyLight 405 NBP2-22127V
DyLight 488 NBP2-22127G
DyLight 550 NBP2-22127R
DyLight 594 NBP2-22127DL594
DyLight 650 NBP2-22127C
DyLight 680 NBP2-22127FR
DyLight 755 NBP2-22127IR
FITC NBP2-22127F
HRP NBP2-22127H
Janelia Fluor 549 NBP2-22127JF549
Janelia Fluor 646 NBP2-22127JF646
PE NBP2-22127PE
PE/Atto594 NBP2-22127PEATT594
PE/Cy5.5 NBP2-22127PECY55
PE/Cy7 NBP2-22127PECY7
PerCP NBP2-22127PCP
Learn about Custom Antibody Labeling

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Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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Bioinformatics

Gene Symbol PRKAA1