Amphiregulin Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Amphiregulin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Amphiregulin
  Ser94-Lys191
  Accession # Q4FJT2
• Specificity: Detects mouse Amphiregulin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AREG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Amphiregulin Antibody (Catalog # AF989)
  ELISA Detection: Mouse Amphiregulin Biotinylated Antibody (Catalog # BAF989)
  Standard: Recombinant Mouse Amphiregulin (Catalog # 989-AR)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiregulin Antibody [Biotin]

• Amphiregulin
• AR
• AREG
• AREGB
• Colorectum cell-derived growth factor
• CRDGF
• MGC13647
• schwannoma-derived growth factor
• SDGF

Background

Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1‑B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.

1. Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
2. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
3. Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Amphiregulin Antibody (BAF989)(15)

Publications using BAF989

• Uddin, J;Yano, H;Parkhurst, CN;Zhang, W;Ahmed, A;Ribeiro de Godoy, V;Wei, Q;Panchakshari, R;Henninot, A;Dasgupta, S;Gaudino, S;Emanuel, ER;Zeng, P;Miranda, I;Hu, E;Tsou, AM;JRI Live Cell Bank, ;Artis, D; CGRP-related neuropeptide adrenomedullin 2 promotes tissue-protective ILC2 responses and limits intestinal inflammation Nature immunology 2025-09-01 [PMID: 40817416] (Flow Cytometry, Mouse)
• Loffredo, LF;Kaiser, KA;Kornberg, A;Rao, S;de Los Santos-Alexis, K;Han, A;Arpaia, N; An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Treg cells bioRxiv : the preprint server for biology 2024-09-28 [PMID: 39386607] (Flow Cytometry, Transgenic Mouse)
• Raugh, A;Jing, Y;Bettini, ML;Bettini, M; The Amphiregulin/EGFR axis has limited contribution in controlling autoimmune diabetes Research square 2023-08-03 [PMID: 37577652] (IHC, Mouse)
• Zhang, Y;Zhang, W;Zhao, J;Ito, T;Jin, J;Aparicio, AO;Zhou, J;Guichard, V;Fang, Y;Que, J;Urban, JF;Hanna, JH;Ghosh, S;Wu, X;Ding, L;Basu, U;Huang, Y; m6A RNA modification regulates innate lymphoid cell responses in a lineage-specific manner Nature immunology 2023-07-03 [PMID: 37400674] (Flow Cytometry, Transgenic Mouse)
• Anastasia du Halgouet, Aurélie Darbois, Mansour Alkobtawi, Martin Mestdagh, Aurélia Alphonse, Virginie Premel et al. Role of MR1-driven signals and amphiregulin on the recruitment and repair function of MAIT cells during skin wound healing Immunity 2023-01-10 [PMID: 36630919]
• AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces Nature, 2022-11-02;0(0):. 2022-11-02 [PMID: 36323781] (Flow Cytometry, Mouse, Transgenic Mouse)
• Karen Agaronyan, Lokesh Sharma, Bharat Vaidyanathan, Keith Glenn, Shuang Yu, Charles Annicelli et al. Tissue remodeling by an opportunistic pathogen triggers allergic inflammation Immunity 2022-05-01 [PMID: 35483356]
• Stanislav Dikiy, Jun Li, Lu Bai, Menglin Jiang, Laura Janke, Xinying Zong et al. A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance Immunity 2021-05-01 [PMID: 33838102]
• Yoshinobu Ikeno, Daiya Ohara, Yusuke Takeuchi, Hitomi Watanabe, Gen Kondoh, Kojiro Taura et al. Foxp3+ Regulatory T Cells Inhibit CCl4-Induced Liver Inflammation and Fibrosis by Regulating Tissue Cellular Immunity Frontiers in Immunology 2020-10-15 [PMID: 33178216]
• B Hu, C Jin, X Zeng, JM Resch, MP Jedrychows, Z Yang, BN Desai, AS Banks, BB Lowell, D Mathis, BM Spiegelman gammadelta T cells and adipocyte IL-17RC control fat innervation and thermogenesis Nature, 2020-02-19;578(7796):610-614. 2020-02-19 [PMID: 32076265] (Flow Cytometry, Mouse)
• Shanti S. D'Souza, Xiaofei Shen, Ivan T. H. Fung, Longyun Ye, Marcy Kuentzel, Sridar V. Chittur et al. Compartmentalized effects of aging on group 2 innate lymphoid cell development and function Aging Cell 2019-12-01 [PMID: 31429526]
• Carlos M. Minutti, Rucha V. Modak, Felicity Macdonald, Fengqi Li, Danielle J. Smyth, David A. Dorward et al. A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation Immunity 2019-03-19 [PMID: 30770250]
• Richard M Jin Regulatory T Cells Promote Myositis and Muscle Damage in Toxoplasma gondii Infection J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895180] (Flow Cytometry, Mouse)
• Alice S Kaanta, Carl Virtanen, Laura M Selfors, Joan S Brugge, Benjamin G Neel Evidence for a multipotent mammary progenitor with pregnancy-specific activity Breast Cancer Research 2013-08-15 [PMID: 23947835] (Flow Cytometry, Mouse)
• Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Lee DC, Prieto J, Avila MA Amphiregulin: an early trigger of liver regeneration in mice. Gastroenterology, 2005-02-01;128(2):424-32. 2005-02-01 [PMID: 15685553] (Western Blot, Rat)

Review for Amphiregulin Antibody (BAF989) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/15/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24315098
• Species: Mouse

Bioinformatics

• Gene Symbol: AREG
• Uniprot:
  • Q4FJT2()