Amphiregulin Antibody (31221)

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Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
31221
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

Amphiregulin Antibody (31221) Summary

Immunogen
E. coli-derived recombinant human Amphiregulin
Specificity
Detects human Amphiregulin (AR) in Western blots. In this format, this antibody shows less than 5% cross-reactivity with recombinant human HB-EGF.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AREG
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 5-15 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human Amphiregulin Biotinylated Antibody (Catalog number BAF262)
Standard: Recombinant Human Amphiregulin (Catalog number 262-AR)
Publications
Read Publications using
MAB262 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiregulin Antibody (31221)

  • Amphiregulin
  • AR
  • AREG
  • AREGB
  • Colorectum cell-derived growth factor
  • CRDGF
  • MGC13647
  • schwannoma-derived growth factor
  • SDGF

Background

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha , HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5-10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Amphiregulin Antibody (MAB262)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA Development, ELISA Microarray Development, ICC, Neutralization, exosome culture.


Filter By Application
ELISA Development
(1)
ELISA Microarray Development
(1)
ICC
(1)
Neutralization
(4)
exosome culture
(1)
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Filter By Species
Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB262 Applications Species
IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional Nature Communications, 2021;12(1):4566. 2021 [PMID: 34315881] (Neutralization, Human) Neutralization Human
TJ Aikin, AF Peterson, MJ Pokrass, HR Clark, S Regot MAPK activity dynamics regulate non-cell autonomous effects of oncogene expression Elife, 2020;9(0):. 2020 [PMID: 32940599] (Neutralization, Mouse) Neutralization Mouse
JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis Cell Rep, 2020;30(11):3806-3820.e6. 2020 [PMID: 32187551] (Neutralization, Human) Neutralization Human
S Raimondo, L Saieva, E Vicario, M Pucci, D Toscani, M Manno, S Raccosta, N Giuliani, R Alessandro Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis J Hematol Oncol, 2019;12(1):2. 2019 [PMID: 30621731] (exosome culture, Human) exosome culture Human
SJ Walker, LM Selfors, BL Margolis, JS Brugge CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin PLoS ONE, 2018;13(11):e0207470. 2018 [PMID: 30440051] (ICC, Human) ICC Human
Stoll S, Stuart P, Lambert S, Gandarillas A, Rittie L, Johnston A, Elder J Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation. J Invest Dermatol, 2016;136(2):444-52. 2016 [PMID: 26802239] (ELISA Development, Human) ELISA Development Human
Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. J. Invest. Dermatol., 2010;130(8):2031-40. 2010 [PMID: 20428186] (Neutralization, Human) Neutralization Human
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008;7(6):2406-14. 2008 [PMID: 18422355] (ELISA Microarray Development, Human) ELISA Microarray Development Human

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Bioinformatics

Gene Symbol AREG