Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

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Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD13 ...read more
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.
Surface staining pattern of human peripheral whole blood stained using anti-human CD13 (WM15) purified antibody (concentration in sample 1 ug/ml, GAM APC).

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready
Clone
WM15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Aminopeptidase N/CD13 Antibody (WM15) - BSA Free Summary

Additional Information
Clone WM15 was used by HLDA to establish CD designation.
Immunogen
Human AML cells (NM_001150.2).
Specificity
The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANPEP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
Application Notes
Functional: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ANPEP