Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD13 Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13.

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.

Surface staining pattern of human peripheral whole blood stained using anti-human CD13 (WM15) purified antibody (concentration in sample 1 ug/ml, GAM APC).

• Reactivity: Hu, Pm
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: WM15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Aminopeptidase N/CD13 Antibody (WM15) - BSA Free Summary

• Description: Novus Biologicals Mouse Aminopeptidase N/CD13 Antibody (WM15) - BSA Free (NB110-58720) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone WM15 was used by HLDA to establish CD designation.
• Immunogen: Human AML cells (NM_001150.2).
• Specificity: The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANPEP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
• Application Notes: Functional: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANPEP