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VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated) in the parental U937 human histiocytic lymphoma cell line, but is not detectable in knockout U937 human histiocytic lymphoma cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
Alanine aminopeptidase (CD13) is expressed on the majority of peripheral blood monocytes and granulocytes. CD13 is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses.
CD13 is commonly used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. Therefore, CD13 antibodies are useful tools for cancer and renal disorder research.
![Immunocytochemistry Aminopeptidase N/CD13 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_AF2335_Immunocytochemistry__Immunofluorescence_17382.jpg)
![Western Blot Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_MAB3815_Western_Blot_19101.jpg)
![N/A Aminopeptidase N/CD13 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Aminopeptidase_N_DY3815_ELISA_1982.jpg)
![Enzyme Activity Aminopeptidase N/CD13 [Unconjugated]](http://images.novusbio.com/fullsize/protein/Aminopeptidase_N_3815ZN_3190.jpg)
![Immunohistochemistry Aminopeptidase N/CD13 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_AF2335_Immunohistochemistry_23316.jpg)
![Immunocytochemistry Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_MAB3815_Immunocytochemistry__Immunofluorescence_19955.jpg)
![Immunohistochemistry Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_MAB3815_Immunohistochemistry_7010.jpg)
![Immunohistochemistry Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/images/antibody/Aminopeptidase_N_MAB3815_Immunohistochemistry_19276.jpg)
![Simple Western Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/Aminopeptidase_N_MAB3815_Simple_Western_22196.jpg)
![Knockout Validated Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]](http://images.novusbio.com/images/antibody/mab3815_human-aminopeptidase-n-cd13-mab-clone-498001-knockout-validated-1662021141218.jpg)
![Binding Activity Aminopeptidase N/CD13 [Biotin]](http://images.novusbio.com/images/avi3815_recombinant-human-aminopeptidase-n-cd13-his-avi-protein-cf-binding-activity-66202493521..jpg)
![Enzyme Activity Aminopeptidase N/CD13 [Biotin]](http://images.novusbio.com/fullsize/avi3815_recombinant-human-aminopeptidase-n-cd13-his-avi-protein-cf-enzyme-activity-234202495137..jpg)
![SDS-Page Aminopeptidase N/CD13 [Biotin]](http://images.novusbio.com/fullsize/avi3815_recombinant-human-aminopeptidase-n-cd13-his-avi-protein-cf-sds-page-224202414306..jpg)
