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Aminopeptidase N/CD13 Antibody (WM15) [FITC]

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Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IP
Clone
WM15
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

Aminopeptidase N/CD13 Antibody (WM15) [FITC] Summary

Additional Information
Clone WM15 was used by HLDA to establish CD designation.
Immunogen
Human AML cells (NM_001150.2).
Specificity
The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANPEP
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.9% Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Aminopeptidase N/CD13 Antibody (NB110-55358). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to know the recommended titer to start flow cytometry with NB110-55358. Would you please help suggest? The customer also noticed that the concentration of this antibody is described as "Predilute mg/ml." We wonder what it means. If the concentration is different from lot to lot. Would you please help inform the conc. of the latest lot?
    • The FITC-conjugated CD13 antibody with catalogue number NB110-55358 can be utilised for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. That the concentration is listed as predilute mg/ml simply means that the product is ready for direct use.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANPEP