alpha-Internexin Antibody (2E3)

Images

 
Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - blot of homogenate of rat facial nucleus incubated with NBP1-05206, which reveals a single clean band running at approx. 66kDa.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - ppocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB 300-140 and GFAP (green), using NB 300-141.
Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Rat facial nucleus before (left) and after (right) facial nerve lesion. Note the profound upregulation of a-internexin in the facial nucleus motor ...read more
Simple Western: alpha-Internexin Antibody (2E3) [NB300-140] - Simple Western lane view shows a specific band for alpha Internexin in 0.5 mg/ml of SH-SY5Y lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
2E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

alpha-Internexin Antibody (2E3) Summary

Immunogen
Purified recombinant rat alpha Internexin [UniProt# P23565]
Epitope
The C-terminal non-helical extension of the protein.
Localization
Cytoplasmic
Marker
Immature Neuronal Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
INA
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Paraffin 1:5000
Application Notes
This alpha Internexin antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. This antibody can be used on formalin-fixed cells in tissue culture and cryostat sections. A 66 kDa band can be seen in Western blotting. The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-140 in the following applications:

  • 8 publications
  • IHC
    13 publications
  • 6 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS with 50% Glycerol and 5 mM Sodium Azide
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for alpha-Internexin Antibody (2E3)

  • 66 kDa neurofilament protein
  • alphaInternexin
  • alpha-Internexin
  • alpha-Inx
  • FLJ18662
  • FLJ57501
  • INA
  • internexin neuronal intermediate filament protein, alpha
  • MGC12702
  • NEF5
  • neurofilament 5 (66kD)
  • Neurofilament 5
  • Neurofilament-66
  • neurofilament-66, tax-binding protein
  • NF-66
  • NF-66alpha-internexin
  • TXBP-1

Background

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-140
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for alpha-Internexin Antibody (NB300-140)(32)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(8)
IHC
(13)
IHC-P
(6)
WB
(5)
All Applications
Filter By Species
Human
(20)
Mouse
(3)
Rat
(6)
Bovine
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB300-140 Applications Species
Bielle F, Di Stefano AL, Meyronnet D et al. Diffuse gliomas with FGFR3-TACC3 fusion have characteristic histopathological and molecular features. Brain Pathol. 2017 Oct 04 [PMID: 28976058] (Human) Human
Breton Q, Plouhinec H, Prunier-Mirebeau D et al. BRAF-V600E immunohistochemistry in a large series of glial and glial-neuronal tumors. Brain Behav. Mar 1 2017 [PMID: 28293477] (IHC, Human) IHC Human
Bielle F, Ducray F, Mokhtari K et al. Tumor cells with neuronal intermediate progenitor features define a subgroup of 1p/19q co-deleted anaplastic gliomas. Brain Pathol 2016 Aug 20 [PMID: 27543943]
Matsumura N, Nobusawa S, Ikota H et al. Coexpression of cyclin D1 and alpha-internexin in oligodendroglial tumors. Brain Tumor Pathol. 2015 Aug 02 [PMID: 26233522] (IHC-P, Human) IHC-P Human
Nagaishi M, Yokoo H, Nobusawa S et al. Localized overexpression of alpha-internexin within nodules in multinodular and vacuolating neuronal tumors Neuropathology. 2015 Jun 14 [PMID: 26073706] (IHC-P, Human) IHC-P Human
Figarella-Branger D, Mokhtari K, Dehais C et al. Mitotic index, microvascular proliferation, and necrosis define 3 groups of 1p/19q codeleted anaplastic oligodendrogliomas associated with different genomic alterations. Neuro-oncology 2014 Apr 09 [PMID: 24723566] (IHC-P, Human) IHC-P Human
Reyes-Botero G, Giry M, Mokhtari K et al. Molecular analysis of diffuse intrinsic brainstem gliomas in adults. J Neurooncol. 2013 Nov 17 [PMID: 24242757] (IHC-P, Human) IHC-P Human
Nagaishi M, Suzuki A, Nobusawa S et al. Alpha-internexin and altered CIC expression as a supportive diagnostic marker for oligodendroglial tumors with the 1p/19q co-deletion. Brain Tumor Pathol. 2013 Nov 7 [PMID: 24197863] (IHC-P, Human) IHC-P Human
Takano S, Kato Y, Yamamoto T et al. Immunohistochemical detection of IDH1 mutation, p53, and internexin as prognostic factors of glial tumors J Neurooncol 2012 Mar 7 [PMID: 22396072] (IHC, Human) IHC Human
Seilhean D, Le Ber I, Sarazin M, Lacomblez L, Millecamps S, Salachas F, Pradat PF, Le Forestier N, LeGuern E, Dubois B, Meininger V, Brice A, Hauw JJ, Duyckaerts C. Fronto-temporal lobar degeneration: neuropathology in 60 cases. J Neural Transm;118(5):753-64. 2011 May. [PMID: 21541771] (IHC, Human) IHC Human
Show All 32 Publications.

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ICC/IF Video Protocol

FAQs for alpha-Internexin Antibody (NB300-140). (Showing 1 - 1 of 1 FAQs).

  1. One of our customers bought the item NB300-140 (lot 9811) in November 2011. In February 2012, the ab was OK, but in March 2012 the antibody was coagulated in the vial. I think it's a contamination of the vial by any cause after opening, and we have no inquiry about a such observation by other customers. Did you have any inquiry about this antibody?
    • I had a look at our stock, and it appears to be fine, though I did notice some precipitate in the bottom of the tubes. I am not sure what this is at this stage, but will run some gels to find out. It should not be cell debris, since the cells are not in the same chamber as the antibody rich supernatant in the CL350 flasks. It may be aggregates of antibody molecules and/or serum proteins. Those tissue culture supernatants can contain very large amounts of antibody, so it would not be too surprising if some antibody precipitated out. I would recommend that your customer centrifuge that material out in the meantime and use the supernatant for their experiments.

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