NF-H Antibody

Images

 
Western Blot: NF-H Antibody [NB300-135] - Different tissue lysates using rabbit pAb to NF-H, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse ...read more
Immunohistochemistry: NF-H Antibody [NB300-135] - Mouse hippocampus section stained with rabbit pAb to NF-H, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in ...read more
Western Blot: 200kDa Neurofilament Heavy Antibody [NB300-135] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NF-H Antibody Summary

Immunogen
Purified bovine Neurofilament Heavy protein.
Marker
Neuronal Marker
Specificity
Phosphorylated and non-phosphorylated forms of 200kDa Neurofilament Heavy
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFH
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Application Notes
This 200kDa Neurofilament Heavy Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, ELISA and Immunohistochemistry on both paraffin-embedded and frozen sections. In Western blot a band can be seen at around 200kDa corresponding to rodent Neurofilament Heavy. In larger species such as porcine or human, Neurofilament Heavy runs a little slower and can be seen around 210-220kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in In vitro assay reported in scientific literature (PMID 27789413).
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NF-H Lysate (NBP2-65371)
Publications
Read Publications using
NB300-135 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for NF-H Antibody

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Heavy is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. Neurofilament Heavy antibodies can be used to identify neurons and their processes in tissue sections and in tissue culture. Additionally, antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-H Antibody (NB300-135)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-P
(1)
In vitro
(1)
WB
(3)
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Filter By Species
Human
(3)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB300-135 Applications Species
Ermilov AN, Kumari A, Li L et al. Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling. PLoS Genet. Nov 1 2016 [PMID: 27893742] (WB, Mouse) WB Mouse
Skrzypczyk A, Giri S, Bader A. Generation of induced pluripotent stem cell line from foreskin fibroblasts Stem Cell Research Sep 15 2016 [PMID: 27789413] (In vitro, Human) In vitro Human
Kumari A, Ermilov An, Allen Bl et al. Hedgehog pathway blockade with the cancer drug LDE225 disrupts taste organs and taste sensation. J. neurophysiol. 2014 Nov 12 [PMID: 25392175]
From R, Eilam R, Bar-Lev DD et al. Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate. Glia 2014 Feb 13 [PMID: 24481644] (IHC-P, Mouse) IHC-P Mouse
Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 2013 Aug 2 [PMID: 23916850] (IHC, Mouse) IHC Mouse
Elan D Louis, Karen, Rachel Babij, Etty Cortes, Ronald K Liem, Jean-Paul G Vonsattel, Phyllis L Faust. Neurofilament Protein Levels: Quantitative Analysis in Essential Tremor Cerebellar Cortex,. Neuroscience Letters, Available online, 10.1016/j.neulet.2012.04.054. 2012 may4. [PMID: 22561033] (WB, IHC, Human) WB, IHC Human
O'Donnell LA, Agrawal A, Sabnekar P et al. Apelin, an endogenous neuronal peptide, protects hippocampal neurons against excitotoxic injury J Neurochem. 2007 Sep [PMID: 17767704] (ICC/IF, Rat) ICC/IF Rat
O Donnell, LA et al. Human immunodeficiency virus (HIV)-induced neurotoxicity roles for the NMDA receptor subtypes. J Neurosci; 26:981-990. 2006 [PMID: 16421318] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-H Antibody (NB300-135). (Showing 1 - 1 of 1 FAQs).

  1. I am using the 200kDa Neurofilament Heavy for the first time. Would it be appropriate to mix it with glycerol for freezer storage rather than aliquot, and do you recommend any antigen retrieval?
    • This product is unpurified whole antisera and should be stable at 4C for few weeks to 6 months, because it contains Sodium Azide as antimicrobial agent (prevents bacterial contamination of the serum). However, if you plan to store it for longer period, you can divide it into aliquots and store the same at -20C or -80C. We do not have a customized protocol for the IHC-P application for this product, however, in general the antigen retrieval step is optional. If you are working on archival tissues or have fixed the tissues for prolonged period, I would highly suggest you to include the antigen retrieval step to your protocol. Louis et al 2012 have cited the use of this antibody for IHC-P application and they have included antigen retrieval step in their experiments. You may follow this paper for more details and images for compression with your study: Louis ED, et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett. 2012 Jun 14;518(1):49-54. Epub 2012 May 4. PMID: 22561033

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEFH
Entrez