Western Blot: 200kDa Neurofilament Heavy Antibody [NB300-135] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the ...read more
Immunocytochemistry/ Immunofluorescence: 200kDa Neurofilament Heavy Antibody [NB300-135] - rat spinal cord stained with Neurofilament Heavy [NF-H] antibody NB300-135 (green) and UCHL1 antibody NB110-58869 (red). ...read more
This 200kDa Neurofilament Heavy Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, ELISA and Immunohistochemistry on both paraffin-embedded and frozen sections. In Western blot a band can be seen at around 200kDa corresponding to rodent Neurofilament Heavy. In larger species such as porcine or human, Neurofilament Heavy runs a little slower and can be seen around 210-220kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in In vitro assay reported in scientific literature (PMID 27789413).
210 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse, rat, bovine, porcine and feline. Most likely to react with all mammals.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for NF-H Antibody
200 kDa neurofilament protein
Neurofilament Heavy (200kDa)
neurofilament heavy polypeptide
Neurofilament triplet H protein
neurofilament, heavy polypeptide 200kDa
neurofilament, heavy polypeptide
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Heavy is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. Neurofilament Heavy antibodies can be used to identify neurons and their processes in tissue sections and in tissue culture. Additionally, antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NF-H Antibody (NB300-135). (Showing 1 - 1 of 1 FAQs).
I am using the 200kDa Neurofilament Heavy for the first time. Would it be appropriate to mix it with glycerol for freezer storage rather than aliquot, and do you recommend any antigen retrieval?
This product is unpurified whole antisera and should be stable at 4C for few weeks to 6 months, because it contains Sodium Azide as antimicrobial agent (prevents bacterial contamination of the serum). However, if you plan to store it for longer period, you can divide it into aliquots and store the same at -20C or -80C. We do not have a customized protocol for the IHC-P application for this product, however, in general the antigen retrieval step is optional. If you are working on archival tissues or have fixed the tissues for prolonged period, I would highly suggest you to include the antigen retrieval step to your protocol. Louis et al 2012 have cited the use of this antibody for IHC-P application and they have included antigen retrieval step in their experiments. You may follow this paper for more details and images for compression with your study: Louis ED, et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett. 2012 Jun 14;518(1):49-54. Epub 2012 May 4. PMID: 22561033
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