NF-H Antibody

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Western Blot: NF-H Antibody [NB300-135] - Proof of viability of organotypic adult brain slices. Brain slices of adult 9 month old wildtype (WT) mice were dissected fresh or incubated for either 1 day or for 14 days to ...read more
Immunohistochemistry: NF-H Antibody [NB300-135] - Mouse hippocampus section stained with rabbit pAb to NF-H, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in ...read more
Immunohistochemistry: NF-H Antibody [NB300-135] - Immunostainings of adult organotypic brain slices at the hippocampal level. Brain slices were prepared from WT or TG adult mice and incubated with okadaic acid (OA). ...read more
Western Blot: NF-H Antibody [NB300-135] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with rabbit ...read more
Western Blot: NF-H Antibody [NB300-135] - Different tissue lysates using rabbit pAb to NF-H, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse ...read more
Proof of viability of organotypic adult brain slices. Brain slices of adult 9 month old wildtype (WT) mice were dissected fresh or incubated for either 1 day or for 14 days to examine vitality of slices (A). Brain ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NF-H Antibody Summary

Immunogen
Purified bovine Neurofilament Heavy protein.
Marker
Neuronal Marker
Specificity
Phosphorylated and non-phosphorylated forms of 200kDa Neurofilament Heavy
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFH
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • In vitro assay
  • Western Blot 1:10000-1:25000
Application Notes
This 200kDa Neurofilament Heavy Antibody is useful for WB, ICC/IF, ELISA and IHC-P, IHC-Frozen. In WB a band can be seen at around 200kDa corresponding to rodent Neurofilament Heavy. In larger species such as porcine or human, Neurofilament Heavy runs a little slower and can be seen around 210-220kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in In vitro assay reported in scientific literature (PMID 27789413). Use in IHC-WhMt reported in scientific literature (PMID:33499508)
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-135 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as serum
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for NF-H Antibody

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Heavy is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. Neurofilament Heavy antibodies can be used to identify neurons and their processes in tissue sections and in tissue culture. Additionally, antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-H Antibody (NB300-135)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC-WhMt, In vitro, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(5)
IHC
(1)
IHC-Fr
(1)
IHC-P
(3)
IHC-WhMt
(2)
In vitro
(1)
WB
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(12)
Rat
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB300-135 Applications Species
Wei SY, Chen PY, Hsieh CC et al. Engineering large and geometrically controlled vascularized nerve tissue in collagen hydrogels to restore large-sized volumetric muscle loss Biomaterials 2023-12-01 [PMID: 37988898] (IHC-P, Mouse) IHC-P Mouse
Manto KM, Govindappa PK, Martinazzi B et al. Erythropoietin-PLGA-PEG as a local treatment to promote functional recovery and neurovascular regeneration after peripheral nerve injury Journal of nanobiotechnology 2022-10-28 [PMID: 36307805] (WB) WB
Korde DS, Humpel C. Spreading of P301S Aggregated Tau Investigated in Organotypic Mouse Brain Slice Cultures Biomolecules 2022-08-23 [PMID: 36139003]
Lee JI, Talukder MAH, Karuman Z et al. Functional recovery and muscle atrophy in pre-clinical models of peripheral nerve transection and gap-grafting in mice: effects of 4-aminopyridine Neural regeneration research 2023-02-01 [PMID: 35900443] (IHC-WhMt, Mouse) IHC-WhMt Mouse
Ucar B, Stefanova N, Humpel C Spreading of Aggregated alpha-Synuclein in Sagittal Organotypic Mouse Brain Slices Biomolecules 2022-01-19 [PMID: 35204664] (WB, Mouse) WB Mouse
Kumari A, Li L, Ermilov AN et al. Unique lingual expression of the Hedgehog pathway antagonist Hedgehog-interacting protein in filiform papillae during homeostasis and ectopic expression in fungiform papillae during Hedgehog signaling inhibition Developmental dynamics : an official publication of the American Association of Anatomists 2022-07-01 [PMID: 35048440] (IHC, Mouse) IHC Mouse
Donnelly CR, Kumari A, Li L et al. Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition Cell and Tissue Research 2022-02-01 [PMID: 34859291]
Liu W, Jiang H, Liu X Et Al. Melatonin Abates TMJOA Chronic Pain by MT2R in Trigeminal Ganglion Neurons Journal of dental research 2021-07-27 [PMID: 34315312]
Manto KM, Govindappa PK, Parisi D Et al. (4-Aminopyridine)PLGAPEG as a Novel Thermosensitive and Locally Injectable Treatment for Acute Peripheral Nerve Injury ACS Appl Bio Mater 2021-06-18 [PMID: 34142019]
Ulger M, Sezer G, Ozyazgan I et al. The effect of erythropoietin and umbilical cord-derived mesenchymal stem cells on nerve regeneration in rats with sciatic nerve injury Journal of chemical neuroanatomy 2021-04-14 [PMID: 33864937] (IF/IHC, Rat) IF/IHC Rat
Show All 28 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-H Antibody (NB300-135). (Showing 1 - 1 of 1 FAQs).

  1. I am using the 200kDa Neurofilament Heavy for the first time. Would it be appropriate to mix it with glycerol for freezer storage rather than aliquot, and do you recommend any antigen retrieval?
    • This product is unpurified whole antisera and should be stable at 4C for few weeks to 6 months, because it contains Sodium Azide as antimicrobial agent (prevents bacterial contamination of the serum). However, if you plan to store it for longer period, you can divide it into aliquots and store the same at -20C or -80C. We do not have a customized protocol for the IHC-P application for this product, however, in general the antigen retrieval step is optional. If you are working on archival tissues or have fixed the tissues for prolonged period, I would highly suggest you to include the antigen retrieval step to your protocol. Louis et al 2012 have cited the use of this antibody for IHC-P application and they have included antigen retrieval step in their experiments. You may follow this paper for more details and images for compression with your study: Louis ED, et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett. 2012 Jun 14;518(1):49-54. Epub 2012 May 4. PMID: 22561033

Secondary Antibodies

 

Isotype Controls

Additional NF-H Products

Research Areas for NF-H Antibody (NB300-135)

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Blogs on NF-H.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

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Bioinformatics

Gene Symbol NEFH
Entrez