Peripherin Antibody

Images

 
Western Blot: Peripherin Antibody [NB300-137] - Analysis of whole rat spinal cord lysate stained with NB300-137, at dilution of 1:1,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa ...read more
Immunocytochemistry/ Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to ...read more
Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Peripherin Antibody Summary

Immunogen
Recombinant full length rat Peripherin [UniProt# P21807]
Localization
Neuronal Marker
Marker
Un-myelinated Neurons Marker
Specificity
Specifically recognizes peripherin, showing no cross reactivity with other Class III intermediate filament proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRPH
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:2000
Application Notes
This Peripherin antibody can be used for ELISA, Immunocytochemistry/Immunofluorescence and Western blotting. In WB a band can be seen at ~57 kDa. Success in IHC on mouse tissue has been reported in a customer review. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 16325747) Use in Immunohistochemistry-frozen reported in scientific literature (PMID 24277578) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-137 in the following applications:

Publications
Read Publications using
NB300-137 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for Peripherin Antibody

  • neurofilament 4 (57kD)
  • Neurofilament 4
  • peripherin
  • PRPH1NEF4

Background

Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Peripherin Antibody (NB300-137)(5)

Review for Peripherin Antibody (NB300-137) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB300-137:
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IHC
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Mao Yang
IHC Mouse 08/17/2010
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Summary

ApplicationImmunohistochemistry
Sample TestedSpinal cord
SpeciesMouse
Commentsworked as expected in IHC after RNA in situ hybridization

Blocking

Blocking DetailsBlocking Buffer: 0.2% casein, Blocking Time: 1 hour, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:500, Incubation Time: overnight, Incubation Temp: 4C, Incubation Dilution Buffer: 0.2% casein TBS tween 20%

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 488, Secondary Ab Dilution Ratio: 1:400

Details

Detection NotesDetection Visualization Method: confocal
Fixation DetailsSample Preparation: OCT Embedded Cryosection

Comments

Commentsworked as expected in IHC after RNA in situ hybridization

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Peripherin Antibody (NB300-137) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Peripherin Antibody (NB300-137)

Discover related pathways, diseases and genes to Peripherin Antibody (NB300-137). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Peripherin Antibody (NB300-137)

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Pathways for Peripherin Antibody (NB300-137)

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Research Areas for Peripherin Antibody (NB300-137)

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Blogs on Peripherin.

A Look at Peripherin: The Unknown Filament
The exact function of Peripherin, or Neurofilament 4, is unknown however it has been suggested to play a role in axon formation and determining and maintaining the shape of nerve cells. Peripherin is a 470 amino acid Class-III neuronal intermediate fi...  Read full blog post.

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Recent Reviews

5
5
1
4
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2
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1
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Mao Yang
08/17/2010
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol PRPH
Entrez