NF-L Antibody


Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse more
Genetic Strategies: Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the more
Immunocytochemistry/ Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal more
Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, more

Product Details

Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Validated by:

Genetic Strategies


Order Details

NF-L Antibody Summary

Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
Neuronal Marker
Specifically recognizes the light neurofilament subunit (~68 kDa).
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Paraffin 1:5000
  • Knockout Validated
  • Western Blot 1:10000-1:15000
Application Notes
This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
NF-L Overexpression Lysate
Read Publications using
NB300-131 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Supplied as serum
5mM Sodium Azide

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa


Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Bv, Ch, Dr, Eq, Hu, Mu, Po, Rt
Applications: IB, ICC/IF, IHC-FrFl, IHC, KD, WB
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ELISA, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, In vitro, WB
Species: Hu, Mu, Rt
Applications: IHC, WB
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: BA
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
Species: Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, KD, Simple Western, WB
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: IHC
Species: Hu, Mu
Applications: IP, WB
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KD, Simple Western, WB
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF, IHC, KO

Publications for NF-L Antibody (NB300-131)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 8 of 8.
Publications using NB300-131 Applications Species
Lin YS, Kuo KT, Chen SK, Huang HS. RBFOX3/NeuN is dispensable for visual function. PLoS ONE. 2018-02-05 [PMID: 29401485] (ICC/IF, Mouse) ICC/IF Mouse
Wang HY, Hsieh PF, Huang DF et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015-12-01 [PMID: 26619789] (WB, Mouse) WB Mouse
From R, Eilam R, Bar-Lev DD et al. Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate. Glia 2014-02-13 [PMID: 24481644] (IHC-P, Mouse) IHC-P Mouse
Huang TN, Chuang HC, Chou WH et al. Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality. Nat. Neurosci. 2014-02-01 [PMID: 24441682] (WB, Mouse) WB Mouse
Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 2013-08-02 [PMID: 23916850] (IF/IHC, Mouse) IF/IHC Mouse
Yokoyama K, Tezuka T, Kotani M a phosphoprotein family that links PI3K to WAVE1 signalling in neurons. The EMBO Journal. 2011-09-23 [PMID: 21946561]
Shim JH, Cho SA, Seo MJ et al. Proteomic analysis of time-dependent difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells. Mol Cells. 2010-03-01 [PMID: 20112072] (ICC/IF, Mouse) ICC/IF Mouse
King AE, Dickson TC, Blizzard CA et al. Neuron-glia interactions underlie ALS-like axonal cytoskeletal pathology Neurobiol Aging 2011-03-01 [PMID: 19427060] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse

Reviews for NF-L Antibody (NB300-131) (0)

There are no reviews for NF-L Antibody (NB300-131). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-L Antibody (NB300-131) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional NF-L Products

Bioinformatics Tool for NF-L Antibody (NB300-131)

Discover related pathways, diseases and genes to NF-L Antibody (NB300-131). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NF-L Antibody (NB300-131)

Discover more about diseases related to NF-L Antibody (NB300-131).

Pathways for NF-L Antibody (NB300-131)

View related products by pathway.

PTMs for NF-L Antibody (NB300-131)

Learn more about PTMs related to NF-L Antibody (NB300-131).

Research Areas for NF-L Antibody (NB300-131)

Find related products by research area.

Blogs on NF-L

There are no specific blogs for NF-L, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our NF-L Antibody and receive a gift card or discount.


Gene Symbol NEFL