NF-L Antibody

Images

 
Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse ...read more
Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, ...read more
Immunocytochemistry/ Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal ...read more
Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NF-L Antibody Summary

Immunogen
Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
Specifically recognizes the light neurofilament subunit (~68 kDa).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Paraffin 1:5000
  • Immunofluorescence
Application Notes
This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NF-L Lysate (NBL1-13577)
Publications
Read Publications using
NB300-131 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-L Antibody (NB300-131)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB300-131 Applications Species
Lin YS, Kuo KT, Chen SK, Huang HS. RBFOX3/NeuN is dispensable for visual function. PLoS ONE. 2018 Feb 05 [PMID: 29401485] (ICC/IF, Mouse) ICC/IF Mouse
Wang HY, Hsieh PF, Huang DF et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015 Dec 01 [PMID: 26619789] (WB, Mouse) WB Mouse
From R, Eilam R, Bar-Lev DD et al. Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate. Glia 2014 Feb 13 [PMID: 24481644] (IHC-P, Mouse) IHC-P Mouse
Huang TN, Chuang HC, Chou WH et al. Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality. Nat. Neurosci. 2014 Jan 29 [PMID: 24441682] (WB, Mouse) WB Mouse
Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 2013 Aug 2 [PMID: 23916850] (IHC, Mouse) IHC Mouse
Yokoyama K, Tezuka T, Kotani M a phosphoprotein family that links PI3K to WAVE1 signalling in neurons. The EMBO Journal. 2011 Sep 23. [PMID: 21946561]
Shim JH, Cho SA, Seo MJ et al. Proteomic analysis of time-dependent difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells. Mol Cells. 2010 Mar [PMID: 20112072] (ICC/IF, Mouse) ICC/IF Mouse
King AE, Dickson TC, Blizzard CA et al. Neuron-glia interactions underlie ALS-like axonal cytoskeletal pathology Neurobiol Aging 2011 Mar [PMID: 19427060] (ICC/IF, IHC, Mouse) ICC/IF, IHC Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEFL
Entrez
Uniprot