NF-L Antibody

Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.

Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355

Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA

Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NF-L Antibody Summary

• Immunogen: Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: Specifically recognizes the light neurofilament subunit (~68 kDa).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEFL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Paraffin 1:5000
  • Knockout Validated
  • Western Blot 1:10000-1:15000
• Application Notes: This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NF-L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as serum
• Preservative: 5mM Sodium Azide
• Purity: Unpurified

Alternate Names for NF-L Antibody

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-L Antibody (NB300-131)(8)

Publications using NB300-131

• Lin YS, Kuo KT, Chen SK, Huang HS. RBFOX3/NeuN is dispensable for visual function. PLoS ONE. 2018-02-05 [PMID: 29401485] (ICC/IF, Mouse)
• Wang HY, Hsieh PF, Huang DF et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015-12-01 [PMID: 26619789] (WB, Mouse)
• From R, Eilam R, Bar-Lev DD et al. Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate. Glia 2014-02-13 [PMID: 24481644] (IHC-P, Mouse)
• Huang TN, Chuang HC, Chou WH et al. Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality. Nat. Neurosci. 2014-02-01 [PMID: 24441682] (WB, Mouse)
• Liu HX, Ermilov A, Grachtchouk M et al. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance. Dev Biol 2013-08-02 [PMID: 23916850] (IF/IHC, Mouse)
• Yokoyama K, Tezuka T, Kotani M a phosphoprotein family that links PI3K to WAVE1 signalling in neurons. The EMBO Journal. 2011-09-23 [PMID: 21946561]
• Shim JH, Cho SA, Seo MJ et al. Proteomic analysis of time-dependent difference of protein expression profile changes during neuronal differentiation of mouse embryonic stem cells. Mol Cells. 2010-03-01 [PMID: 20112072] (ICC/IF, Mouse)
• King AE, Dickson TC, Blizzard CA et al. Neuron-glia interactions underlie ALS-like axonal cytoskeletal pathology Neurobiol Aging 2011-03-01 [PMID: 19427060] (ICC/IF, IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: NEFL
• Entrez:
  • 4747(Human)
  • 18039(Mouse)
  • 83613(Rat)
• Uniprot:
  • P02547(Porcine)