NF-M Antibody


Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain more
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled more
Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ch, Dr, EqSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-FrFl, KD

Order Details

NF-M Antibody Summary

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.
Neuronal Marker
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000
  • Knockdown Validated
  • Western Blot 1:10000
Application Notes
This 160kDa Neurofilament Medium antibody is useful for ICC/IF, IHC and Western blot. In Western blot this antibody detects a band at ~145-160kDa. Knockdown validation (PMID: 31811140). Use in immunoblotting reported in scientific literature (PMID:31811140).
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
NF-M Overexpression Lysate
Read Publications using
NB300-222 in the following applications:

  • IB
    1 publication
  • 7 publications
  • KD
    1 publication
  • WB
    1 publication

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID:31811140).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Supplied as concentrated IgY in PBS
0.02% Sodium Azide
IgY purified


Chicken products cannot be exported to Canada.

Alternate Names for NF-M Antibody

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein


Neuronal intermediate filaments (NIF) are type-IV intermediate filaments found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. Mature NIFs are heteropolymers with about 4:2:1 stoichiometric ratio of NEFL: NEFM: NEFH and also contain a fourth type-IV intermediate filament (IF) subunit, alpha-internexin. NEFM is essential to cross-bridge formation, stabilization, and longitudinal extension of filamentous network and is more critical than NEFH in regulating NIF's structure and function. NEFM is crucial for the acquisition of normal axonal caliber in response to a myelin-dependent "outside-in" trigger for radial axonal growth and removal of the tail domain as well as lysine-serine-proline (KSP) repeats of NEFM, but not NEFH, produces axons with impaired radial growth and reduced conduction velocities. Moreover, mutations of NEFM have been associated with CNS/PNS disorders such as ALS, Parkinson's disease etc., and an imbalance in NEFM's O-GlcNAcylation - phosphorylation regulation plays a crucial role in in Alzheimer disease.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ELISA, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, In vitro, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
Species: Hu, Mu, Rt
Applications: IHC, WB
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
Species: Hu, Mu
Applications: IHC
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KD, Simple Western, WB
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt, Po, Bv, Ch, Dr, Eq
Applications: WB, IB, ICC/IF, IHC, IHC-FrFl, KD

Publications for NF-M Antibody (NB300-222)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 4 applications: IB, ICC/IF, KD, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB300-222 Applications Species
Batool S, Akhter B, Zaidi J Et al. Neuronal Menin Overexpression Rescues Learning and Memory Phenotype in CA1-Specific alpha 7 nAChRs KD Mice Cells Nov 24 2021 [PMID: 34943798] (ICC/IF, Mouse) ICC/IF Mouse
Batool S, Zaidi J, Akhter B et al. Spatiotemporal Patterns of Menin Localization in Developing Murine Brain: Co-Expression with the Elements of Cholinergic Synaptic Machinery Cells May 16 2021 [PMID: 34065662] (ICC/IF, Mouse) ICC/IF Mouse
Casci I, Krishnamurthy K, Kour S Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization Nat Commun Dec 6 2019 [PMID: 31811140] (ICC/IF, IB, KD, Rat, Human, Drosophila) ICC/IF, IB, KD Rat, Human, Drosophila
Chu TH, Cummins K, Sparling JS et al. Axonal and myelinic pathology in 5xFAD Alzheimer's mouse spinal cord. PLoS ONE 2017 Nov 27 [PMID: 29176903] (Mouse) Mouse
Getz AM, Xu F, Visser F et al. Tumor suppressor menin is required for subunit-specific nAChR a5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons. Sci Rep May 11 2017 [PMID: 28496137] (ICC/IF, Mouse) ICC/IF Mouse
Xu F, Armstrong R, Urrego D et al. The mitochondrial division inhibitor Mdivi-1 rescues mammalian neurons from anesthetic-induced cytotoxicity Mol Brain Mar 24 2016 [PMID: 27009068] (ICC/IF, Rat) ICC/IF Rat
Gold M, Mengel D, Roskam S, Dodel R, Bach JP. Mechanisms of action of naturally occurring antibodies against beta-amyloid on microglia. J Neuroinflammation 2013 Jan 14 [PMID: 23317003] (WB, Mouse) WB Mouse
Portmann-Lanz CB, Schoeberlein A, Portmann R et al. Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes. Am J Obstet Gynecol. 2010 Mar [PMID: 20060088] (ICC/IF, Human) ICC/IF Human
Iqbal F, Pehar M, Thompson A et al. A synthetic peptide rescues rat cortical neurons from anesthetic-induced cell death and modulation of growth and synaptic assembly bioRxiv Jun 20 2020
Iqbal F, Pehar M, Thompson AJ et al. A synthetic peptide rescues rat cortical neurons from anesthetic-induced cell death, perturbation of growth and synaptic assembly Scientific reports Feb 25 2021 [PMID: 33633281] (ICC/IF, Rat) ICC/IF Rat

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Gene Symbol NEFM