NF-M Antibody

Images

 
Western Blot: 160kDa Neurofilament Medium Antibody [NB300-222] - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NB300-222, which reveals a strong NF-M band at ...read more
Immunocytochemistry/ Immunofluorescence: 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fe, FiSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

NF-M Antibody Summary

Immunogen
The C-terminal extension of rat Neurofilament Medium protein (the so-called KE segment) [UniProt# P12839]
Marker
Neuronal Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NEFM
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:2000
Application Notes
This 160kDa Neurofilament Medium antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot this antibody detects a band at ~145-170kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NF-M Lysate (NBL1-13578)
Reviewed Applications
Read 1 Review rated 5
using
NB300-222 in the following applications:

Publications
Read Publications using
NB300-222 in the following applications:

Reactivity Notes

Human, mouse, rat, fish, cat and chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
10mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for NF-M Antibody

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neuronal intermediate filaments (NIF) are type-IV intermediate filaments found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. Mature NIFs are heteropolymers with about 4:2:1 stoichiometric ratio of NEFL: NEFM: NEFH and also contain a fourth type-IV intermediate filament (IF) subunit, alpha-internexin. NEFM is essential to cross-bridge formation, stabilization, and longitudinal extension of filamentous network and is more critical than NEFH in regulating NIF's structure and function. NEFM is crucial for the acquisition of normal axonal caliber in response to a myelin-dependent "outside-in" trigger for radial axonal growth and removal of the tail domain as well as lysine-serine-proline (KSP) repeats of NEFM, but not NEFH, produces axons with impaired radial growth and reduced conduction velocities. Moreover, mutations of NEFM have been associated with CNS/PNS disorders such as ALS, Parkinson's disease etc., and an imbalance in NEFM's O-GlcNAcylation - phosphorylation regulation plays a crucial role in in Alzheimer disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-M Antibody (NB300-222)(4)

Review for NF-M Antibody (NB300-222) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NB300-222:
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ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry NF-M NB300-222
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reviewed by:
Karthik Krishnamurthy
ICC Rat 10/17/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedCortical neurons
SpeciesRat
Lot1-0104

Comments

CommentsConfocal microscope image of cultured rat cortical (14 days invitro) neurons fixed with 4% paraformaldehyde and stained for Neurofilament (red) and MAP-2 (green). Neurofilament antibody was used at a dilution of 1:1000 and incubated at 4 degrees overnight.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-M Antibody (NB300-222) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NF-M Antibody (NB300-222)

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Blogs on NF-M

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Recent Reviews

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1
4
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2
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1
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Karthik Krishnamurthy
10/17/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol NEFM
Entrez
Uniprot