Neurofascin Antibody

Images

 
Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal ...read more
Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Neurofascin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
Ile25-Ala1031
Accession # NP_446361
Specificity
Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs.
Source
N/A
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NFASC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3235 in the following applications:

Publications
Read Publications using
AF3235 in the following applications:

  • ICC
    1 publication
  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurofascin Antibody

  • DKFZp686P2250
  • KIAA0756FLJ46866
  • neurofascin homolog (chicken)
  • neurofascin homolog
  • Neurofascin
  • NF
  • NFASC
  • NRCAML

Background

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

  1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
  3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  5. Collinson, J.M. et al. (1998) Glia 23:11.
  6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
  9. Sherman, D.L. et al. (2005) Neuron 48:737.
  10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
  11. Charles, P. et al. (2002) Curr. Biol. 12:217.
  12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for Neurofascin Antibody (AF3235)(3)

Review for Neurofascin Antibody (AF3235) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3235:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
YONGJIE LAI
IF Human 07/16/2015
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Summary

ApplicationImmunofluorescence
Sample TestedHEK 293 cell line transfected with Neurofascin expression plasmid
SpeciesHuman
LotCFCT0214081

Blocking

Blocking Details5% normal donkey serum in PBS 1 HOUR RT

Primary Anitbody

Dilution Ratio1:200 dilution in blocking buffer overnight at 4 degrees

Details

Detection NotesFluorescence microscopy
Fixation Detailsfixed with 4% PFA for 5 minutes

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Neurofascin Antibody (AF3235). (Showing 1 - 1 of 1 FAQs).

  1. Does the neurofascin antibody #AF3235 detect both 155 and 186 isoforms?
    • The immunogen for this antibody is "Mouse myeloma cell line NS0-derived recombinant rat Neurofascin Ile25 - Ala1031". It means that any protein target has the the partial or full-length region that is included in Ile25 - Ala1031 will be recognized because it is a polyclonal antibody. Then, I checked the rat sequence posting on UniProt (http://www.uniprot.org/uniprot/P97685#sequences), it says that rat has three Neurofascin isoforms: 1 to 3. Isoform has 1,240 a.a. with a mass of 138,004 (Da), whereas both isoforms 2 and 3 are known as "NF155, 155 kDa isoforms" According above information, the isoforms 2 and 3 should also be recognized by the antibody, AF3235, because the missing and sequence-substituted regions are not excluded out of "Ile25 - Ala1031"; and there are still a lot of epitopes left on the protein species for binding. Unfortunately, I did not locate any information on isoform 186 (assuming this isoform has ~186 kDa) in rat, human Uniprot, or mouse Uniprot databases. If you have the a.a. sequence for the isoform, you could analyze the structure and determine whether AF3235 can recognize it.

Secondary Antibodies

 

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YONGJIE LAI
07/16/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NFASC
Entrez
Uniprot