Carboxylesterase 2/CES2 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI-N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carboxylesterase 2/CES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5657) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 2/CES2 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Carboxylesterase 2/CES2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Carboxylesterase 2/CES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5657) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxylesterase 2/CES2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase 2/CES2
  Gln27-Leu559
  Accession # O00748
• Specificity: Detects human Carboxylesterase 2/CES2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CES1, recombinant mouse (rm) CES2, rmCES3, and rmCES5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CES2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxylesterase 2/CES2 Antibody [Unconjugated]

• carboxylesterase 2 (intestine, liver)
• Carboxylesterase 2
• carboxylesterase 2EC 3.1.1.1
• CE-2
• CE-2PCE-2
• CES2
• CES2A1
• EC 3.1.1
• EC 3.1.1.84
• hCE-2
• iCE
• intestinal carboxylesterase; liver carboxylesterase-2
• PCE-2

Background

CES2 (Carboxylesterase 2; also CE-2) is a glycosylated, monomeric member of the type-B carboxyesterease/lipase family of enzymes. Although it runs at 59‑62 kDa in SDS-PAGE, Gel filtration assigns an 80 kDa MW to it. CES2 is an ER luminal enzyme that catalyzes the conversion of carboxlic ester to alcohol. It is found in small intestine and liver, and likely participates in the detoxification of drugs. Mature human CES2 is 533 amino acids (aa) in length (aa 27-559). It contains a catalytic site composed of Ser228Glu345His457, and an ER-retention motif over aa 556 - 559. Individually or in combination, splice variants may show an alternate start site 64 aa upstream of the standard site, a Val substitution for aa 458-474, or a nine aa insertion after Met1. Over the length of the precursor (aa 1-559) human CES2 shares 72% aa identity with mouse CES2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carboxylesterase 2/CES2 Antibody (AF5657)(1)

Publication using AF5657

• N Yamada, R Negoro, K Watanabe, T Fujita Generation of Caco-2�cells with predictable metabolism by CYP3A4, UGT1A1 and CES using the PITCh system Drug metabolism and pharmacokinetics, 2023-02-18;50(0):100497. 2023-02-18 [PMID: 37037169] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CES2
• Uniprot:
  • O00748()