Serpin C1/Antithrombin-III Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin C1/Antithrombin-III Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin C1/Antithrombin-III
  His33-Lys464
  Accession # P01008
• Specificity: Detects human Serpin C1/Antithrombin-III in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse (rm) Serpin C1 is observed and less than 2% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin F2, rmSerpin D1 and rhPEDF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINC1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 4.6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin C1/Antithrombin-III Antibody [Unconjugated]

• Antithrombin-III
• AT3antithrombin-III
• ATIII
• ATIIIantithrombin III
• MGC22579
• serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• serine-cysteine proteinase inhibitor clade C member 1
• Serpin C1
• serpin peptidase inhibitor, clade C (antithrombin), member 1

Background

Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
2. Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
3. Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
4. Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin C1/Antithrombin-III Antibody (AF1267)(3)

Publications using AF1267

• RJ Weiss, PN Spahn, AWT Chiang, Q Liu, J Li, KM Hamill, S Rother, TM Clausen, MA Hoeksema, BM Timm, K Godula, CK Glass, Y Tor, PLSM Gordts, NE Lewis, JD Esko Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate Nature Chemical Biology, 2021-04-12;0(0):. 2021-04-12 [PMID: 33846619] (Flow Cytometry, Human)
• A Gerasimov, V Golderman, SG Gofrit, SA Aharoni, DN Zohar, Z Itsekson-H, T Fay-Karmon, S Hassin-Bae, J Chapman, N Maggio, E Shavit-Ste Markers for neural degeneration and regeneration: novel highly sensitive methods for the measurement of thrombin and activated protein C in human cerebrospinal fluid Neural regeneration research, 2021-10-01;16(10):2086-2092. 2021-10-01 [PMID: 33642398] (Western Blot, Human)
• Tseng IC, Chou FP, Su SF, Oberst M, Madayiputhiya N, Lee MS, Wang JK, Sloane DE, Johnson M, Lin CY Purification from human milk of matriptase complexes with secreted serpins: mechanism for inhibition of matriptase other than HAI-1. Am. J. Physiol., Cell Physiol., 2008-06-11;295(2):C423-31. 2008-06-11 [PMID: 18550704] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPINC1
• Uniprot:
  • P01008()