Serpin A3N Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A3N Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
  Phe21-Lys418
  Accession # Q91WP6
• Specificity: Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Serpina3n
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A3N Antibody [Unconjugated]

• A1A;A1AT;AAT;Alpha-1-antitrypsin;alpha1AT;nNIF;PI;PI1;PRO2275;SERPINA1
• Serpin A3N
• Spi2

Background

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

1. Forsyth, S. et al. (2003) Genomics 81:336.
2. Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
3. Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A3N Antibody (AF4709)(34)

Publications using AF4709

• Mittra, N;He, S;Bao, H;Bhattacharjee, A;Dodds, SG;Dupree, JL;Han, X; Sulfatide deficiency-induced astrogliosis and myelin lipid dyshomeostasis are independent of Trem2-mediated microglial activation bioRxiv : the preprint server for biology 2024-11-14 [PMID: 39605561] (Western Blot, Transgenic Mouse)
• Aubert, A;Liu, A;Kao, M;Goeres, J;Richardson, KC;Nierves, L;Jung, K;Nabai, L;Zhao, H;Orend, G;Krawetz, R;Lange, PF;Younger, A;Chan, J;Granville, DJ; Granzyme B cleaves Tenascin-C to release its C-terminal domain in rheumatoid arthritis JCI insight 2024-10-17 [PMID: 39475853] (Western Blot, Human)
• Koupourtidou, C;Schwarz, V;Aliee, H;Frerich, S;Fischer-Sternjak, J;Bocchi, R;Simon-Ebert, T;Bai, X;Sirko, S;Kirchhoff, F;Dichgans, M;Götz, M;Theis, FJ;Ninkovic, J; Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex Nature communications 2024-04-03 [PMID: 38570482] (Immunohistochemistry, Mouse)
• J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination Cell Reports, 2023-03-21;42(4):112293. 2023-03-21 [PMID: 36952346] (IHC, Mouse)
• Y Zhou, M Tada, Z Cai, PS Andhey, A Swain, KR Miller, S Gilfillan, MN Artyomov, M Takao, A Kakita, M Colonna Human early-onset dementia caused by DAP12 deficiency reveals a unique signature of dysregulated microglia Nature Immunology, 2023-01-19;0(0):. 2023-01-19 [PMID: 36658241] (IHC, Mouse)
• Melanie Tran, Md Golam Mostofa, Michael Picard, Jianguo Wu, Li Wang, Dong-Ju Shin SerpinA3N deficiency attenuates steatosis and enhances insulin signaling in male mice Journal of Endocrinology 2023-03-01 [PMID: 36625462]
• NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron Microglia regulate central nervous system myelin growth and integrity Nature, 2022-12-14;0(0):. 2022-12-14 [PMID: 36517604] (IHC, Mouse)
• NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron Microglia regulate central nervous system myelin growth and integrity Nature, 2022;0(0):. 2022 [PMID: 36517604] (IHC, Mouse)
• J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease Nature, 2022-11-16;0(0):. 2022-11-16 [PMID: 36385530] (IHC, Mouse)
• T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging Nature Neuroscience, 2022-10-24;25(11):1446-1457. 2022-10-24 [PMID: 36280798] (IHC, Mouse)
• Y Shen, W Yang, X Xiong, X Li, Z Xiao, J Yu, F Liu, S Gui, X Xie, F Lv, L Zhao, L Hu, A Manaenko, P Xie, Q Li Integrated Multiomics Analysis Identifies a Novel Biomarker Associated with Prognosis in Intracerebral Hemorrhage Oxidative Medicine and Cellular Longevity, 2021-12-14;2021(0):2510847. 2021-12-14 [PMID: 36226158] (Western Blot, Mouse)
• M Kenigsbuch, P Bost, S Halevi, Y Chang, S Chen, Q Ma, R Hajbi, B Schwikowsk, B Bodenmille, H Fu, M Schwartz, I Amit A shared disease-associated oligodendrocyte signature among multiple CNS pathologies Nature Neuroscience, 2022-06-27;0(0):. 2022-06-27 [PMID: 35760863] (IHC, Mouse)
• Sivaprakasam R. Saroja, Abhijeet Sharma, Patrick R. Hof, Ana C. Pereira Differential expression of tau species and the association with cognitive decline and synaptic loss in Alzheimer's disease Alzheimer's & Dementia 2022-09-01 [PMID: 34873815]
• S Qiu, JP Palavicini, J Wang, NS Gonzalez, S He, E Dustin, C Zou, L Ding, A Bhattachar, CE Van Skike, V Galvan, JL Dupree, X Han Adult-onset CNS myelin sulfatide deficiency is sufficient to cause Alzheimer's disease-like neuroinflammation and cognitive impairment Molecular Neurodegeneration, 2021-09-15;16(1):64. 2021-09-15 [PMID: 34526055] (IHC, Mouse)
• Hai-Tao Jiang, Rui Deng, Yan Deng, Mao Nie, Yi-Xuan Deng, Hong-Hong Luo et al. The role of Serpina3n in the reversal effect of ATRA on dexamethasone-inhibited osteogenic differentiation in mesenchymal stem cells Stem Cell Research & Therapy 2021-12-01 [PMID: 34001245]
• YT Ho, T Shimbo, E Wijaya, T Kitayama, S Takaki, K Ikegami, K Miyashita, Y Ouchi, E Takaki, R Yamamoto, Y Kaneda, K Tamai Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model Cellular and Molecular Gastroenterology and Hepatology, 2021-04-13;0(0):. 2021-04-13 [PMID: 33862275] (IHC, Immunoprecipitation, Western Blot, Mouse)
• MM Akbor, N Kurosawa, H Nakayama, A Nakatani, K Tomobe, Y Chiba, M Ueno, M Tanaka, Y Nomura, M Isobe Polymorphic SERPINA3 prolongs oligomeric state of amyloid beta PLoS ONE, 2021-03-04;16(3):e0248027. 2021-03-04 [PMID: 33662018] (IHC, Mouse)
• Gregory J. Heller, Michael S. Marshall, Yazan Issa, Jeffrey N. Marshall, Duc Nguyen, Emily Rue et al. Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease Molecular Therapy 2021-05-01 [PMID: 33508430]
• Nicolás Fissolo, Clara Matute-Blanch, Mohamoud Osman, Carme Costa, Rucsanda Pinteac, Berta Miró et al. CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS Neurology - Neuroimmunology Neuroinflammation 2021-03-01 [PMID: 33436375]
• E Staurenghi, V Cerrato, P Gamba, G Testa, S Giannelli, V Leoni, C Caccia, A Buffo, W Noble, BG Perez-Niev, G Leonarduzz Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2 Redox Biology, 2020-12-17;39(0):101837. 2020-12-17 [PMID: 33360775] (Western Blot, Mouse)
• IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia Cancers (Basel), 2020-10-31;12(11):. 2020-10-31 [PMID: 33142864] (Western Blot, Mouse)
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020-08-14;11(1):4102. 2020-08-14 [PMID: 32796823] (IHC, Mouse)
• JJ Hulmi, F Penna, N Pöllänen, TA Nissinen, J Hentilä, L Euro, JH Lautaoja, R Ballarò, R Soliymani, M Baumann, O Ritvos, E Pirinen, M Lalowski Muscle NAD+ depletion and Serpina3n as molecular determinants of murine cancer cachexia - the effects of blocking myostatin and activins Mol Metab, 2020-06-26;0(0):101046. 2020-06-26 [PMID: 32599075] (Western Blot, Mouse)
• N Habib, C McCabe, S Medina, M Varshavsky, D Kitsberg, R Dvir-Szter, G Green, D Dionne, L Nguyen, JL Marshall, F Chen, F Zhang, T Kaplan, A Regev, M Schwartz Disease-associated astrocytes in Alzheimer's disease and aging Nat. Neurosci., 2020-04-27;23(6):701-706. 2020-04-27 [PMID: 32341542] (IHC, Mouse)
• Na Zhao, Yingxue Ren, Yu Yamazaki, Wenhui Qiao, Fuyao Li, Lindsey M. Felton et al. Alzheimer’s Risk Factors Age, APOE Genotype, and Sex Drive Distinct Molecular Pathways Neuron 2020-06-01 [PMID: 32199103]
• DA Lipski, V Foucart, R Dewispelae, LE Caspers, M Defrance, C Bruyns, F Willermain Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach BMC Ophthalmol, 2020-03-17;20(1):106. 2020-03-17 [PMID: 32183784] (IF, Mouse)
• Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease Nat. Med., 2020-01-13;26(1):131-142. 2020-01-13 [PMID: 31932797] (IHC, Mouse)
• Domenico Sergi, Fiona M. Campbell, Christine Grant, Amanda C. Morris, Eva-Maria Bachmair, Christiane Koch et al. SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice Genes & Nutrition 2018-12-01 [PMID: 30519364]
• MJ Dalby, G Aviello, AW Ross, AW Walker, P Barrett, PJ Morgan Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N Sci Rep, 2018-10-23;8(1):15648. 2018-10-23 [PMID: 30353127] (IHC, Mouse)
• Marine Gueugneau, Donatienne d'Hose, Caroline Barbé, Marie de Barsy, Pascale Lause, Dominique Maiter et al. Increased Serpina3n release into circulation during glucocorticoid-mediated muscle atrophy Journal of Cachexia, Sarcopenia and Muscle 2018-10-01 [PMID: 29989354]
• S Vanni, F Moda, M Zattoni, E Bistaffa, E De Cecco, M Rossi, G Giaccone, F Tagliavini, S Haïk, JP Deslys, G Zanusso, JW Ironside, I Ferrer, GG Kovacs, G Legname Differential overexpression of SERPINA3 in human prion diseases Sci Rep, 2017-11-15;7(1):15637. 2017-11-15 [PMID: 29142239] (Western Blot, Mouse)
• Vicuna L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin R, Njoo C, Gehrig S, Mall M, Arnold B, Devor M, Woolf C, Liberles S, Costigan M, Kuner R The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. Nat Med, 2015-04-27;21(5):518-23. 2015-04-27 [PMID: 25915831] (IHC, Western Blot, Mouse)
• I Hsu, L G Parkinson, Y Shen, A Toro, T Brown, H Zhao et al. Serpina3n accelerates tissue repair in a diabetic mouse model of delayed wound healing Cell Death & Disease 2014-10-09 [PMID: 25299783]
• Williams , Lynda M, Campbell , Fiona M, Drew , Janice E, Koch , Christia, Hoggard , Nigel, Rees , William, Kamolrat , Torkamol, Thi Ngo , Ha, Steffensen , Inger-Li, Gray , Stuart R, Tups , Alexande The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases. PLoS ONE, 2014-08-29;9(8):e106159. 2014-08-29 [PMID: 25170916] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Serpina3n
• Uniprot:
  • Q91WP6(Mouse)