AIF-1/Iba1 Antibody (rAIF1/1909) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Po, RMSpecies Glossary
Applications IHC, IHC-P
Clone
rAIF1/1909
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

AIF-1/Iba1 Antibody (rAIF1/1909) [DyLight 650] Summary

Immunogen
AIF-1/Iba1 Antibody (rAIF1/1909) was developed against purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Localization
Cytoplasmic and Cell Surface
Predicted Species
Porcine (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AIF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for AIF-1/Iba1 Antibody (rAIF1/1909) [DyLight 650]

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75760AF350
Alexa Fluor 405 NBP2-75760AF405
Alexa Fluor 488 NBP2-75760AF488
Alexa Fluor 532 NBP2-75760AF532
Alexa Fluor 594 NBP2-75760AF594
Alexa Fluor 647 NBP2-75760AF647
Alexa Fluor 700 NBP2-75760AF700
Alexa Fluor 750 NBP2-75760AF750
Biotin NBP2-75760B
DyLight 350 NBP2-75760UV
DyLight 405 NBP2-75760V
DyLight 488 NBP2-75760G
DyLight 550 NBP2-75760R
DyLight 594 NBP2-75760DL594
DyLight 650 NBP2-75760C
DyLight 680 NBP2-75760FR
DyLight 755 NBP2-75760IR
FITC NBP2-75760F
HRP NBP2-75760H
Janelia Fluor 549 NBP2-75760JF549
Janelia Fluor 646 NBP2-75760JF646

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Bioinformatics

Gene Symbol AIF1