AIF-1/Iba1 Antibody (1022-5)

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IA, IHC, IHC-P
Clone
1022-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

AIF-1/Iba1 Antibody (1022-5) Summary

Immunogen
This product was made using a portion of the human IBA1 gene.
Marker
pan-Microglia Marker
Specificity
The monoclonal antibody 1022-5 is cross reactive with rat Allograft-inflammatory factor-1 (rat AIF-1). Human and rat amino acids sequences are 98% homologous.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AIF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50
  • Immunoassay 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This antibody works in Immunoassay, Immunohistochemistry-Paraffin, Western Blot. For immunohistology on paraffin sections, a combination of microwave and sodium citrate solution is recommended for antigen retrieval.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-50156 in the following applications:

Publications
Read Publication using
NBP1-50156 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27036089).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for AIF-1/Iba1 Antibody (1022-5)

  • AIF1
  • AIF-1
  • AIF-1IRT1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IBA1Em:AF129756.17
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NBP1-50156)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Rat
(1)
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Review for AIF-1/Iba1 Antibody (NBP1-50156) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP1-50156:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen AIF-1/Iba1 NBP1-50156
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reviewed by:
Victoria Andriulis
IHC-Fr Mouse 08/09/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested4% PFA fixed frozen sections from mouse brain
SpeciesMouse
Lot20442MO716-A

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Secondary Antibodies

 

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Bioinformatics Tool for AIF-1/Iba1 Antibody (NBP1-50156)

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Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Victoria Andriulis
08/09/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot