Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (1022-5) [NBP1-50156] - Analysis using the FITC conjugate of NBP1-50156. Staining of human spleen. Immunohistochemistry of Staining of tissue after heat-induced antigen ...read more
0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin
0.02% Sodium Azide
Protein A or G purified
Western Blot 1:50
This antibody works in Immunoassay, Immunohistochemistry-Paraffin, Western Blot. For immunohistology on paraffin sections, a combination of microwave and sodium citrate solution is recommended for antigen retrieval.
Read Publication using NBP1-50156 in the following applications:
Rat reactivity reported in scientific literature (PMID: 27036089).
Alternate Names for AIF-1/Iba1 Antibody (1022-5)
allograft inflammatory factor 1
interferon gamma responsive transcript
Ionized calcium-binding adapter molecule 1
Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.