AgRP/ART Antibody (82815)

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 82815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

AgRP/ART Antibody (82815) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP
  Ala21-Thr132
  Accession # O00253
• Specificity: Detects human AgRP in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant mouse AgRP.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGRP
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human AgRP/ART Biotinylated Antibody (Catalog number BAF704)
  Standard: Recombinant Human AgRP/ART (Catalog number 704-AG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AgRP/ART Antibody (82815)

• agouti related protein homolog (mouse)
• agouti-related protein
• AgRP
• Agrt
• AGRTMGC118963
• ART
• ARTagouti (mouse) related protein
• ASIP2

Background

AgRP, also known as the protein product of the Agouti Related Transcript (ART), functions as an antagonist of the melanocortin (MC-3 and MC-4) receptors, both of which are implicated in obesity. AgRP levels are significantly elevated in the serum of obese mice and people.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGRP
• Uniprot:
  • O00253()