CART/CARTPT Antibody [Unconjugated]

CART was detected in immersion fixed paraffin-embedded sections of human brain (Hypothalamus) tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CART was detected in perfusion fixed frozen sections of mouse brain tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. Staining was performed using our Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CART/CARTPT Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CART
  Gln28-Leu116
  Accession # Q16568
• Specificity: Detects human CART in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CARTPT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CART/CARTPT Antibody [Unconjugated]

• CART prepropeptide
• CART
• CARTcocaine and amphetamine regulated transcript
• CARTPT
• cocaine- and amphetamine-regulated transcript protein

Background

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1‑89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

1. Douglass, J. & S. Daoud (1996) Gene 169:241.
2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
3. Adams, L.D. et al. (1999) Brain Res. 848:137.
4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
7. Thim, L. et al. (1998) FEBS Lett. 428:263.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CART/CARTPT Antibody (AF163)(7)

Publications using AF163

• Harima, Y;Tsurutani, M;Yamada, S;Uchida, S;Inada, K;Hagihara, M;Irie, S;Shigeta, M;Abe, T;Inoue, YU;Inoue, T;Miyamichi, K; Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice Nature communications 2024-12-10 [PMID: 39658565] (Immunohistochemistry, Mouse)
• Kobori, N;Moore, AN;Redell, JB;Dash, PK; Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function Journal of neuroinflammation 7/4/2023 [PMID: 37403174]
• Topilko T, Diaz SL, Pacheco CM et al. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting Neuron 2/1/2022 [PMID: 35123655]
• Lyla Kelley, Silvanna Verlezza, Hong Long, Mary Loka, Claire-Dominique Walker Increased Hypothalamic Projections to the Lateral Hypothalamus and Responses to Leptin in Rat Neonates From High Fat Fed Mothers Frontiers in Neuroscience 1/31/2020 [PMID: 32082105]
• Laurent Gautron Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges eNeuro, 2016-10-05;3(5):. 10/5/2016 [PMID: 27822503]
• Ye Long, Andrea S. Bordt, Weiley S. Liu, Elizabeth P. Davis, Stephen J. Lee, Luke Tseng et al. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART) Peptides 10/1/2016 [PMID: 27568514]
• Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus. PLoS ONE, 2013-04-26;8(4):e62532. 2013-04-26 [PMID: 23638108] (ICC, IHC, Rat)

Bioinformatics

• Gene Symbol: CARTPT
• Uniprot:
  • Q16568()