Melanocortin-3 R/MC3R Antibody

Western Blot: Melanocortin-3 R/MC3R Antibody [NB100-1501] - (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Melanocortin-3 R/MC3R Antibody Summary

• Immunogen: Peptide with sequence SIQKTYLEGDFV-C corresponding to N-Terminus according to NP_063941.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MC3R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human brain lysates (calculated MW of 40.1 kDa band according to NP_063941.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Melanocortin-3 R/MC3R Antibody

• BMIQ9
• MC3
• MC3R
• MC3-R
• melanocortin 3 receptor
• melanocortin receptor 3
• Melanocortin-3 R
• Melanocortin3R
• Melanocortin-3R
• OB20
• Obesity quantitative trait locus
• OQTL

Background

Melanocortins are ligands for a subgroup of GPCRs positively coupled to Adenylate cylcases, termed as melanocortin receptors (MC1R, MC2R, MC3R, MC4R amd MC5R). These receptors are widely expressed at varied density throughout the body. The MC3R has been widely implicated in inflammation and is also implicated in a role in obesity and metabolism. MC3R KO mice exhibit reduced linear growth and lean mass and are hyperleptinemic and hyperinsulinemic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melanocortin-3 R/MC3R Antibody (NB100-1501)(1)

Publication using NB100-1501

• Muhlhausler B, Adam C, Marrocco E et al. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005-05-15 [PMID: 15661821]

Review for Melanocortin-3 R/MC3R Antibody (NB100-1501) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Quentin Howlett-Prieto Flow Human 02/05/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Blood mononuclear cells (PBMCs)
• Species: Human
• Lot: P1

Comments

• Comments: Blocking: Natural Mouse Serum
  Primary antibody: 4 uL (2 ug)
  Secondary antibody: Alexa Fluor 488 Rabbit anti-Goat IgG (2.5 uL / .5 ug)
  
  Details: Human lymphocytes were isolated from PBMCs. Lymphocytes were blocked with Natural Mouse Serum for 15 minutes, and then stained and subsequently incubated at -20 C with anti-human MC3R primary antibody for 30 minutes. After washing, cells were stained with Alexa Fluor 488 Rabbit anti-Goat IgG and incubated at -20 C for 40 minutes. After washing, cells were detected using a Fortessa 4-15 flow cytometer.

Bioinformatics

• Gene Symbol: MC3R
• Entrez:
  • 4159(Human)
• Uniprot:
  • NP_063941.(Human)